A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system
The current study provides a way of extraction for both active NSO and WSE from Nigella sativa seeds using 98% methanol. About 1 kg of ground seeds was macerated by 1:2.5 w/v (g/mL) for 72 hours. After rotary evaporation and 7 days of continuous drying and chilling at 50 and 4 °C, NSO and WSE were o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Taylor and Francis
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/65108/ http://irep.iium.edu.my/65108/ http://irep.iium.edu.my/65108/ http://irep.iium.edu.my/65108/7/65108-A%20simple%20method%20for%20extracting%20both%20active%20oily%20-in-press.pdf http://irep.iium.edu.my/65108/9/65108%20A%20simple%20method%20for%20extracting%20both%20active%20oily%20and%20water%20soluble%20extract%20SCOPUS-in-press.pdf |
| Summary: | The current study provides a way of extraction for both active NSO and WSE from Nigella sativa seeds using 98% methanol. About 1 kg of ground seeds was macerated by 1:2.5 w/v (g/mL) for 72 hours. After rotary evaporation and 7 days of continuous drying and chilling at 50 and 4 °C, NSO and WSE were obtained at the same instant. Solubility tests of 24 solvents and 11 thin layer chromatographic analyses while 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging assay of NSO (73.66) , WSE (33.32) and NSO + WSE (78.22) against ascorbic acid (IC50 = 4.28 mg/mL) was performed. WSE was found to be highly soluble in water and 5% NaOH exhibiting the same Rf value of 0.95 for EtOH:DMSO (9:1) against the honey. WSE has revealed more than twofold higher anti-oxidant activity than others. Formulation of WSE with Tualang honey may provide better targeted hydrophilic drug delivery systems. |
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