Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract

Medicinal plants are commonly used in complementary and alternative medicine to treat several chronic diseases. Gynura procumbens, Hibiscus sabdariffa and Clinacanthus nutans are believed to contain high level of antioxidants including phenolic acids that may contribute to antioxidant activities. Th...

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Main Authors:	Haron, Normah, Muhamad Bunnori, Noraslinda, Md Zin, Noor Hasniza, Abdul Wahab, Widya, Abd Halim, Khairul Bariyyah, Mat Jusoh, Hanapi
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/64822/ http://irep.iium.edu.my/64822/1/Poster-Format-MRS%20KRD%282%29%20Dr.%20Normah%20Haron.pdf http://irep.iium.edu.my/64822/17/64822%20Quantification%20of%20total%20phenolic.pdf

id	iium-64822
recordtype	eprints
spelling	iium-648222019-08-23T07:21:25Z http://irep.iium.edu.my/64822/ Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract Haron, Normah Muhamad Bunnori, Noraslinda Md Zin, Noor Hasniza Abdul Wahab, Widya Abd Halim, Khairul Bariyyah Mat Jusoh, Hanapi QD Chemistry Medicinal plants are commonly used in complementary and alternative medicine to treat several chronic diseases. Gynura procumbens, Hibiscus sabdariffa and Clinacanthus nutans are believed to contain high level of antioxidants including phenolic acids that may contribute to antioxidant activities. Thus, the objectives of this study were to determine total phenolic content (TPC) and antioxidant scavenging capacity of these plants using three different extracts as well as to determine the relationship between TPC and antioxidant activity. The quantification of phenolic compound was conducted by analyzing the compounds at three different fractions (free, insoluble bound and soluble bound). Three replicates of the medicinal plants were extracted with 80% methanol (free fraction) and undergone hydrolysis with HCl (soluble bound) and NaOH (insoluble bound). The TPC was determined using Folin-Ciocalteau reagent while antioxidant radical scavenging capacities were analyzed using DPPH assay. Results showed that G. procumbens and C. nutans of the insoluble bound extracts had the highest amount of TPC compared to the free phenolic and soluble bound (p<0.05). Meanwhile, H. sabdariffa had the highest TPC value in soluble bound extract (7.63 + 0.28 mg GAE/g) compared to the free phenolic and insoluble bound extracts (2.78 + 0.20 mg GAE/g and 3.61 + 0.85 mg GAE/g, respectively) (p<0.05). The antioxidant scavenging capacity of the G. procumbens and C. nutans extracts as determined by IC50 values was the highest in the insoluble bound extract followed by free phenolic and soluble bound extracts (p<0.05). In contrast, the soluble bound extract of H. sabdariffa showed the highest IC50 value followed by free phenolic and insoluble bound extracts. We also found strong correlations between TPC with antioxidant radical scavenging capacity. In conclusion, this finding suggests that these plants possess a good source of natural antioxidants, which could be used as an non-conventional substances in the management of inflammatory-related diseases. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/64822/1/Poster-Format-MRS%20KRD%282%29%20Dr.%20Normah%20Haron.pdf application/pdf en http://irep.iium.edu.my/64822/17/64822%20Quantification%20of%20total%20phenolic.pdf Haron, Normah and Muhamad Bunnori, Noraslinda and Md Zin, Noor Hasniza and Abdul Wahab, Widya and Abd Halim, Khairul Bariyyah and Mat Jusoh, Hanapi (2016) Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Haron, Normah Muhamad Bunnori, Noraslinda Md Zin, Noor Hasniza Abdul Wahab, Widya Abd Halim, Khairul Bariyyah Mat Jusoh, Hanapi Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
description	Medicinal plants are commonly used in complementary and alternative medicine to treat several chronic diseases. Gynura procumbens, Hibiscus sabdariffa and Clinacanthus nutans are believed to contain high level of antioxidants including phenolic acids that may contribute to antioxidant activities. Thus, the objectives of this study were to determine total phenolic content (TPC) and antioxidant scavenging capacity of these plants using three different extracts as well as to determine the relationship between TPC and antioxidant activity. The quantification of phenolic compound was conducted by analyzing the compounds at three different fractions (free, insoluble bound and soluble bound). Three replicates of the medicinal plants were extracted with 80% methanol (free fraction) and undergone hydrolysis with HCl (soluble bound) and NaOH (insoluble bound). The TPC was determined using Folin-Ciocalteau reagent while antioxidant radical scavenging capacities were analyzed using DPPH assay. Results showed that G. procumbens and C. nutans of the insoluble bound extracts had the highest amount of TPC compared to the free phenolic and soluble bound (p<0.05). Meanwhile, H. sabdariffa had the highest TPC value in soluble bound extract (7.63 + 0.28 mg GAE/g) compared to the free phenolic and insoluble bound extracts (2.78 + 0.20 mg GAE/g and 3.61 + 0.85 mg GAE/g, respectively) (p<0.05). The antioxidant scavenging capacity of the G. procumbens and C. nutans extracts as determined by IC50 values was the highest in the insoluble bound extract followed by free phenolic and soluble bound extracts (p<0.05). In contrast, the soluble bound extract of H. sabdariffa showed the highest IC50 value followed by free phenolic and insoluble bound extracts. We also found strong correlations between TPC with antioxidant radical scavenging capacity. In conclusion, this finding suggests that these plants possess a good source of natural antioxidants, which could be used as an non-conventional substances in the management of inflammatory-related diseases.
format	Conference or Workshop Item
author	Haron, Normah Muhamad Bunnori, Noraslinda Md Zin, Noor Hasniza Abdul Wahab, Widya Abd Halim, Khairul Bariyyah Mat Jusoh, Hanapi
author_facet	Haron, Normah Muhamad Bunnori, Noraslinda Md Zin, Noor Hasniza Abdul Wahab, Widya Abd Halim, Khairul Bariyyah Mat Jusoh, Hanapi
author_sort	Haron, Normah
title	Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_short	Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_full	Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_fullStr	Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_full_unstemmed	Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_sort	quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
publishDate	2016
url	http://irep.iium.edu.my/64822/ http://irep.iium.edu.my/64822/1/Poster-Format-MRS%20KRD%282%29%20Dr.%20Normah%20Haron.pdf http://irep.iium.edu.my/64822/17/64822%20Quantification%20of%20total%20phenolic.pdf
first_indexed	2023-09-18T21:32:01Z
last_indexed	2023-09-18T21:32:01Z
_version_	1777412573555064832

Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract

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