Quantification of phenolic compounds in selected herbs using HPLC

The present study was conducted to profile the presence of selected individual phenolic compounds samples namely Citrus hystrix, Coriandrum sativum, Murayya koenigii, Polygonum minus, Ocimum basilicum and Anacardium occidentale) in three different fractions (free, soluble bound and insoluble bound)...

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Main Authors:	Mohd Yusof, Nor Amaliana Syazwani, Abdullah Thaidi, Nur Imanina, Haron, Normah, Mat Jusoh, Hanapi, Muhamad Bunnori, Noraslinda
Format:	Conference or Workshop Item
Language:	English English
Published:	2018
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/64808/ http://irep.iium.edu.my/64808/ http://irep.iium.edu.my/64808/7/64808%20Quantification%20of%20Phenolic%20-Abstract.pdf http://irep.iium.edu.my/64808/1/Poster-ICNP%202018%20Dr.%20Normah%20Haron%20C%20design.pdf

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spelling	iium-648082018-07-27T07:25:23Z http://irep.iium.edu.my/64808/ Quantification of phenolic compounds in selected herbs using HPLC Mohd Yusof, Nor Amaliana Syazwani Abdullah Thaidi, Nur Imanina Haron, Normah Mat Jusoh, Hanapi Muhamad Bunnori, Noraslinda QD Chemistry The present study was conducted to profile the presence of selected individual phenolic compounds samples namely Citrus hystrix, Coriandrum sativum, Murayya koenigii, Polygonum minus, Ocimum basilicum and Anacardium occidentale) in three different fractions (free, soluble bound and insoluble bound) using optimized HPLC conditions with C6-Phenyl column. The concentrations of each compound were quantified by the calibration curves with R2=0.999. The results showed that caffeic acid, sinapinic acid, p-coumaric acid and gallic acid were the common phenolic acids found in all fractions. Caffeic acid was the main compound extracted from the free phenolic fraction of C. hystrix (87.34 ± 103.94 µg/g of extract), while sinapinic acid was found in the highest concentration in soluble and insoluble bounds (117.69 ± 10.19 and 25.89 ± 1.29 µg/g of extract), respectively. In C. sativum, the highest concentration of caffeic acid was seen in the insoluble bound extract (461.41 ± 94.66 µg/g of extract) (p< 0.05). For M. koenigii, sinapinic acid was the major compound detected in free phenolic extract and insoluble bound extract (271.17 ± 22.74 and 106.57 ± 5.79 µg/g of extract), respectively. Gallic acid was predominated in the soluble bound of P. minus extract (391.83 ± 37.43 µg/g of extract) with p<0.05. Highest concentrations of caffeic acid were found in all fractions of O. basilicum extract (soluble bound, insoluble bound and free phenolic: 442.60 ± 10.78, 342.21 ± 63.30 and 18.99 ± 0.42 µg/g of extract, respectively) with p<0.05. In conclusion, the hydroxycinnamic acid is dominated in the plant as compared to hydroxybenzoic acids. 2018 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/64808/7/64808%20Quantification%20of%20Phenolic%20-Abstract.pdf application/pdf en http://irep.iium.edu.my/64808/1/Poster-ICNP%202018%20Dr.%20Normah%20Haron%20C%20design.pdf Mohd Yusof, Nor Amaliana Syazwani and Abdullah Thaidi, Nur Imanina and Haron, Normah and Mat Jusoh, Hanapi and Muhamad Bunnori, Noraslinda (2018) Quantification of phenolic compounds in selected herbs using HPLC. In: 34th International Conference on Natural Products 2018 (ICNP 2018), 19th-21st March 2018, Bayview Beach Resort, Batu Ferringhi, Penang. (Unpublished) http://www.icnp2018.usm.my/images/ICNP/E-Abstract/ICNP-2018-E-Book.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Mohd Yusof, Nor Amaliana Syazwani Abdullah Thaidi, Nur Imanina Haron, Normah Mat Jusoh, Hanapi Muhamad Bunnori, Noraslinda Quantification of phenolic compounds in selected herbs using HPLC
description	The present study was conducted to profile the presence of selected individual phenolic compounds samples namely Citrus hystrix, Coriandrum sativum, Murayya koenigii, Polygonum minus, Ocimum basilicum and Anacardium occidentale) in three different fractions (free, soluble bound and insoluble bound) using optimized HPLC conditions with C6-Phenyl column. The concentrations of each compound were quantified by the calibration curves with R2=0.999. The results showed that caffeic acid, sinapinic acid, p-coumaric acid and gallic acid were the common phenolic acids found in all fractions. Caffeic acid was the main compound extracted from the free phenolic fraction of C. hystrix (87.34 ± 103.94 µg/g of extract), while sinapinic acid was found in the highest concentration in soluble and insoluble bounds (117.69 ± 10.19 and 25.89 ± 1.29 µg/g of extract), respectively. In C. sativum, the highest concentration of caffeic acid was seen in the insoluble bound extract (461.41 ± 94.66 µg/g of extract) (p< 0.05). For M. koenigii, sinapinic acid was the major compound detected in free phenolic extract and insoluble bound extract (271.17 ± 22.74 and 106.57 ± 5.79 µg/g of extract), respectively. Gallic acid was predominated in the soluble bound of P. minus extract (391.83 ± 37.43 µg/g of extract) with p<0.05. Highest concentrations of caffeic acid were found in all fractions of O. basilicum extract (soluble bound, insoluble bound and free phenolic: 442.60 ± 10.78, 342.21 ± 63.30 and 18.99 ± 0.42 µg/g of extract, respectively) with p<0.05. In conclusion, the hydroxycinnamic acid is dominated in the plant as compared to hydroxybenzoic acids.
format	Conference or Workshop Item
author	Mohd Yusof, Nor Amaliana Syazwani Abdullah Thaidi, Nur Imanina Haron, Normah Mat Jusoh, Hanapi Muhamad Bunnori, Noraslinda
author_facet	Mohd Yusof, Nor Amaliana Syazwani Abdullah Thaidi, Nur Imanina Haron, Normah Mat Jusoh, Hanapi Muhamad Bunnori, Noraslinda
author_sort	Mohd Yusof, Nor Amaliana Syazwani
title	Quantification of phenolic compounds in selected herbs using HPLC
title_short	Quantification of phenolic compounds in selected herbs using HPLC
title_full	Quantification of phenolic compounds in selected herbs using HPLC
title_fullStr	Quantification of phenolic compounds in selected herbs using HPLC
title_full_unstemmed	Quantification of phenolic compounds in selected herbs using HPLC
title_sort	quantification of phenolic compounds in selected herbs using hplc
publishDate	2018
url	http://irep.iium.edu.my/64808/ http://irep.iium.edu.my/64808/ http://irep.iium.edu.my/64808/7/64808%20Quantification%20of%20Phenolic%20-Abstract.pdf http://irep.iium.edu.my/64808/1/Poster-ICNP%202018%20Dr.%20Normah%20Haron%20C%20design.pdf
first_indexed	2023-09-18T21:31:59Z
last_indexed	2023-09-18T21:31:59Z
_version_	1777412572100689920

Quantification of phenolic compounds in selected herbs using HPLC

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