Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis

Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis

Osteoarthritis (OA) is a degenerative joint disease that is characterized by subchondral bone changes. Previous studies of OA have shown that structural changes were differed between subchondral bone plate (Sbp) and trabecular bone (Tb). However, the expression of OPG (bone formation) and RANKL (...

Full description

Bibliographic Details
Main Authors: Akman, Akma Azmiera, Talib, Ain Aisyah, Md Yusof, Nur Azirah, Khalid, Kamarul Ariffin, Yusof, Nurul Asyiqin, Abdul Ghani, Radiah, Hamdan, Asmah Hanim, Zamli, Zaitunnatakhin
Format: Conference or Workshop Item
Language:English
Published: 2018
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/64806/
http://irep.iium.edu.my/64806/1/MSAB%202018%20abstract.pdf
id iium-64806
recordtype eprints
spelling iium-648062019-03-14T02:31:25Z http://irep.iium.edu.my/64806/ Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis Akman, Akma Azmiera Talib, Ain Aisyah Md Yusof, Nur Azirah Khalid, Kamarul Ariffin Yusof, Nurul Asyiqin Abdul Ghani, Radiah Hamdan, Asmah Hanim Zamli, Zaitunnatakhin R Medicine (General) Osteoarthritis (OA) is a degenerative joint disease that is characterized by subchondral bone changes. Previous studies of OA have shown that structural changes were differed between subchondral bone plate (Sbp) and trabecular bone (Tb). However, the expression of OPG (bone formation) and RANKL (bone resorption) markers that underlying the differences between these two regions has yet to be determined. Hence, the present study aims to determine structural changes and expressions of OPG/RANKL in the Sbp and Tb during development of OA in Dunkin Hartley guinea pigs. Eighteen tibia was scanned using a micro-computed tomography (micro-CT) at three difference tome points; 10, 20 and 30 weeks of age to determine their Sbp and Tb thickness. Immunohistochemistry and histopathology methods were conducted to determine the expression of OPG/RANKL and microscopic OA score, respectively. The results showed that Sbp and Tb thickness were greater in the medial than the lateral side of tibial plateau, and increased significantly with ageing (p ≤ 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp was initially decreased, before being increased at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. Interestingly, a strong correlation was observed between the Sbp thickness and OPG/RANKL ratio with microscopic OA scores (r = 0.5, p ≤ 0.05), suggesting that the Sbp plays an important role in the pathogenesis of OA. 2018-07-01 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/64806/1/MSAB%202018%20abstract.pdf Akman, Akma Azmiera and Talib, Ain Aisyah and Md Yusof, Nur Azirah and Khalid, Kamarul Ariffin and Yusof, Nurul Asyiqin and Abdul Ghani, Radiah and Hamdan, Asmah Hanim and Zamli, Zaitunnatakhin (2018) Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis. In: The 15th Symposium of the Malaysian Society of Applied Biology (MSAB 2018), 29th June - 1st July 2018, Melaka. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Akman, Akma Azmiera
Talib, Ain Aisyah
Md Yusof, Nur Azirah
Khalid, Kamarul Ariffin
Yusof, Nurul Asyiqin
Abdul Ghani, Radiah
Hamdan, Asmah Hanim
Zamli, Zaitunnatakhin
Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis
description Osteoarthritis (OA) is a degenerative joint disease that is characterized by subchondral bone changes. Previous studies of OA have shown that structural changes were differed between subchondral bone plate (Sbp) and trabecular bone (Tb). However, the expression of OPG (bone formation) and RANKL (bone resorption) markers that underlying the differences between these two regions has yet to be determined. Hence, the present study aims to determine structural changes and expressions of OPG/RANKL in the Sbp and Tb during development of OA in Dunkin Hartley guinea pigs. Eighteen tibia was scanned using a micro-computed tomography (micro-CT) at three difference tome points; 10, 20 and 30 weeks of age to determine their Sbp and Tb thickness. Immunohistochemistry and histopathology methods were conducted to determine the expression of OPG/RANKL and microscopic OA score, respectively. The results showed that Sbp and Tb thickness were greater in the medial than the lateral side of tibial plateau, and increased significantly with ageing (p ≤ 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp was initially decreased, before being increased at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. Interestingly, a strong correlation was observed between the Sbp thickness and OPG/RANKL ratio with microscopic OA scores (r = 0.5, p ≤ 0.05), suggesting that the Sbp plays an important role in the pathogenesis of OA.
format Conference or Workshop Item
author Akman, Akma Azmiera
Talib, Ain Aisyah
Md Yusof, Nur Azirah
Khalid, Kamarul Ariffin
Yusof, Nurul Asyiqin
Abdul Ghani, Radiah
Hamdan, Asmah Hanim
Zamli, Zaitunnatakhin
author_facet Akman, Akma Azmiera
Talib, Ain Aisyah
Md Yusof, Nur Azirah
Khalid, Kamarul Ariffin
Yusof, Nurul Asyiqin
Abdul Ghani, Radiah
Hamdan, Asmah Hanim
Zamli, Zaitunnatakhin
author_sort Akman, Akma Azmiera
title Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_short Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_full Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_fullStr Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_full_unstemmed Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis
title_sort structural changes and the differential expression of osteoprotegerin (opg) and receptor activator of nuclear factor kb ligand (rankl) in subchondral bone during the development of osteoarthritis
publishDate 2018
url http://irep.iium.edu.my/64806/
http://irep.iium.edu.my/64806/1/MSAB%202018%20abstract.pdf
first_indexed 2023-09-18T21:31:59Z
last_indexed 2023-09-18T21:31:59Z
_version_ 1777412571800797184

Similar Items