Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kb ligand (RANKL) in subchondral bone during the development of osteoarthritis
Osteoarthritis (OA) is a degenerative joint disease that is characterized by subchondral bone changes. Previous studies of OA have shown that structural changes were differed between subchondral bone plate (Sbp) and trabecular bone (Tb). However, the expression of OPG (bone formation) and RANKL (...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/64806/ http://irep.iium.edu.my/64806/1/MSAB%202018%20abstract.pdf |
| Summary: | Osteoarthritis (OA) is a degenerative joint disease that is characterized by subchondral bone
changes. Previous studies of OA have shown that structural changes were differed between
subchondral bone plate (Sbp) and trabecular bone (Tb). However, the expression of OPG
(bone formation) and RANKL (bone resorption) markers that underlying the differences
between these two regions has yet to be determined. Hence, the present study aims to
determine structural changes and expressions of OPG/RANKL in the Sbp and Tb during
development of OA in Dunkin Hartley guinea pigs. Eighteen tibia was scanned using a
micro-computed tomography (micro-CT) at three difference tome points; 10, 20 and 30
weeks of age to determine their Sbp and Tb thickness. Immunohistochemistry and
histopathology methods were conducted to determine the expression of OPG/RANKL and
microscopic OA score, respectively. The results showed that Sbp and Tb thickness were
greater in the medial than the lateral side of tibial plateau, and increased significantly with
ageing (p ≤ 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp
was initially decreased, before being increased at the final time point. In contrast, the
OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age.
Interestingly, a strong correlation was observed between the Sbp thickness and OPG/RANKL
ratio with microscopic OA scores (r = 0.5, p ≤ 0.05), suggesting that the Sbp plays an
important role in the pathogenesis of OA. |
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