Oleoyl-lysophosphatidylinositol enhances glucagon-like peptide-1 secretion from enteroendocrine L-cells through GPR119
The gastrointestinal tract is increasingly viewed as critical in controlling glucose metabolism, because of its role in secreting multiple glucoregulatory hormones, such as glucagon like peptide-1 (GLP-1). Here we investigate the molecular pathways behind the GLP-1- and insulin-secreting capabilitie...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Elsevier
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/64800/ http://irep.iium.edu.my/64800/ http://irep.iium.edu.my/64800/ http://irep.iium.edu.my/64800/7/64800_Oleoyl-lysophosphatidylinositol%20enhances_SCOPUS.pdf http://irep.iium.edu.my/64800/13/64800_Oleoyl-lysophosphatidylinositol%20enhances.pdf http://irep.iium.edu.my/64800/19/64800_Oleoyl-lysophosphatidylinositol%20enhances%20_WoS.pdf |
| Summary: | The gastrointestinal tract is increasingly viewed as critical in controlling glucose metabolism, because of its role in secreting multiple glucoregulatory hormones, such as glucagon like peptide-1 (GLP-1). Here we investigate the molecular pathways behind the GLP-1- and insulin-secreting capabilities of a novel GPR119 agonist, Oleoyl-lysophosphatidylinositol (Oleoyl-LPI). Oleoyl-LPI is the only LPI species able to potently stimulate the release of GLP-1 in vitro, from murine and human L-cells, and ex-vivo from murine colonic primary cell preparations. Here we show that Oleoyl-LPI mediates GLP-1 secretion through GPR119 as this activity is ablated in cells lacking GPR119 and in colonic primary cell preparation from GPR119-/- mice. Similarly, Oleoyl-LPI-mediated insulin secretion is impaired in islets isolated from GPR119-/- mice. On the other hand, GLP-1 secretion is not impaired in cells lacking GPR55 in vitro or in colonic primary cell preparation from GPR55-/- mice. We therefore conclude that GPR119 is the Oleoyl-LPI receptor, upstream of ERK1/2 and cAMP/PKA/CREB pathways, where primarily ERK1/2 is required for GLP-1 secretion, while CREB activation appears dispensable |
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