Cinnamtannin B1: a small compound having antidiabetes properties

Type 2 diabetes mellitus is a chronic metabolic disease that results from defects in insulin secretion and insulin receptor kinase. Investigation of novel small active molecule that can potentiate insulin action or having a similar action as insulin is important in the treatment of diabetes. World...

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Main Authors:	Bakhtiar, M. Taher, Abdul Majid, Fadzilah Adibah, Sarmidi, Mohamad Roji
Other Authors:	Nejati, Mostafa
Format:	Book Chapter
Language:	English
Published:	Universal Publisher 2009
Subjects:	RM283 Endocrinotheraphy RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/6474/ http://irep.iium.edu.my/6474/1/ebook_iomc.pdf

id	iium-6474
recordtype	eprints
spelling	iium-64742014-12-09T00:39:30Z http://irep.iium.edu.my/6474/ Cinnamtannin B1: a small compound having antidiabetes properties Bakhtiar, M. Taher Abdul Majid, Fadzilah Adibah Sarmidi, Mohamad Roji RM283 Endocrinotheraphy RS Pharmacy and materia medica Type 2 diabetes mellitus is a chronic metabolic disease that results from defects in insulin secretion and insulin receptor kinase. Investigation of novel small active molecule that can potentiate insulin action or having a similar action as insulin is important in the treatment of diabetes. World ethnobotanical information on medicinal plants reports almost 800 plants used in the treatment of diabetes mellitus. However, only a small number of them have been studied thoroughly. Recent study conducted on Cinnamomum reported that it has a great activity in activating insulin receptor kinase and inhibiting insulin receptor phosphatase leading to increased insulin sensitivity and function as a mimetic for insulin. Our study was designed to investigate insulin-mimetic activity of cinnamtannin B1 isolated from Cinnamomum zeylanicum on adipocyte cells. The insulin-mimetic activity of cinnamtannin B1 was evaluated by monitoring preadipocytes differentiation, glucose uptake and phosphorylation of insulin receptor β-subunit in 3T3-L1 adipocytes. To determine whether cinnamtannin B1 able to promote differentiation of preadipocytes, we cultured 3T3-L1 preadipocytes in the presence of cinnamtannin B1, or combination of cinnamtannin B1 and insulin, and then cell proliferation was measured at several points during the course of growth. Investigation of role of cinnamtannin B1 on tyrosine posphorylation of insulin receptor of 3T3-L1 cells was done by immunoprecipitation of cells lysate with anti-insulin receptor β-subunit antibody and the immunocomplex samples were subjected to SDS-PAGE, transferred to nitrocelluose membranes, and immunoblotted with monoclonal anti-phosphotyrosine antibody. Evaluation of glucose uptake by adipocyte cells after treatment with cinnamtannin B1 was carried out by analyzing of radioactive glucose uptake with liquid scintillation counter. Based on these experiments, it was found that, a mixture cinnamtannin B1 with differentiation cocktail was able to induce differentiation of preadipocytes cells. Cinnamtannin B1 was found to active to stimulate phosphorylation of insulin receptor β-subunit by positively exhibited phosphorylation at 170-kDa. The mixture of cinnamtannin B1 was also able to stimulate glucose uptake from a basal value. The results demonstrated that activity of cinnamtannin B1 on adipocyte cells was found to mimicking insulin action. It acted directly on insulin receptor β-subunit by activation of PI3-kinase that stimulates glucose transporter-4 (GLUT-4) translocation. Stimulation of GLUT4 translocation therefore stimulates glucose uptake lead to glucose disposal process in adipocytes. Based on the work that has been carried out, it was suggested that cinnamtannin B1 could be one of the Annals of Medicine & Healthcare Research Page 452 potential lead drug compound in the treatment of type 2 diabetes. Universal Publisher Nejati, Mostafa Forouzan Bayat, Nejad 2009 Book Chapter NonPeerReviewed application/pdf en http://irep.iium.edu.my/6474/1/ebook_iomc.pdf Bakhtiar, M. Taher and Abdul Majid, Fadzilah Adibah and Sarmidi, Mohamad Roji (2009) Cinnamtannin B1: a small compound having antidiabetes properties. In: Annals of Medicine & Healthcare Research. Universal Publisher, Boca Raton, Florida, pp. 450-465. ISBN 9781599429083
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM283 Endocrinotheraphy RS Pharmacy and materia medica
spellingShingle	RM283 Endocrinotheraphy RS Pharmacy and materia medica Bakhtiar, M. Taher Abdul Majid, Fadzilah Adibah Sarmidi, Mohamad Roji Cinnamtannin B1: a small compound having antidiabetes properties
description	Type 2 diabetes mellitus is a chronic metabolic disease that results from defects in insulin secretion and insulin receptor kinase. Investigation of novel small active molecule that can potentiate insulin action or having a similar action as insulin is important in the treatment of diabetes. World ethnobotanical information on medicinal plants reports almost 800 plants used in the treatment of diabetes mellitus. However, only a small number of them have been studied thoroughly. Recent study conducted on Cinnamomum reported that it has a great activity in activating insulin receptor kinase and inhibiting insulin receptor phosphatase leading to increased insulin sensitivity and function as a mimetic for insulin. Our study was designed to investigate insulin-mimetic activity of cinnamtannin B1 isolated from Cinnamomum zeylanicum on adipocyte cells. The insulin-mimetic activity of cinnamtannin B1 was evaluated by monitoring preadipocytes differentiation, glucose uptake and phosphorylation of insulin receptor β-subunit in 3T3-L1 adipocytes. To determine whether cinnamtannin B1 able to promote differentiation of preadipocytes, we cultured 3T3-L1 preadipocytes in the presence of cinnamtannin B1, or combination of cinnamtannin B1 and insulin, and then cell proliferation was measured at several points during the course of growth. Investigation of role of cinnamtannin B1 on tyrosine posphorylation of insulin receptor of 3T3-L1 cells was done by immunoprecipitation of cells lysate with anti-insulin receptor β-subunit antibody and the immunocomplex samples were subjected to SDS-PAGE, transferred to nitrocelluose membranes, and immunoblotted with monoclonal anti-phosphotyrosine antibody. Evaluation of glucose uptake by adipocyte cells after treatment with cinnamtannin B1 was carried out by analyzing of radioactive glucose uptake with liquid scintillation counter. Based on these experiments, it was found that, a mixture cinnamtannin B1 with differentiation cocktail was able to induce differentiation of preadipocytes cells. Cinnamtannin B1 was found to active to stimulate phosphorylation of insulin receptor β-subunit by positively exhibited phosphorylation at 170-kDa. The mixture of cinnamtannin B1 was also able to stimulate glucose uptake from a basal value. The results demonstrated that activity of cinnamtannin B1 on adipocyte cells was found to mimicking insulin action. It acted directly on insulin receptor β-subunit by activation of PI3-kinase that stimulates glucose transporter-4 (GLUT-4) translocation. Stimulation of GLUT4 translocation therefore stimulates glucose uptake lead to glucose disposal process in adipocytes. Based on the work that has been carried out, it was suggested that cinnamtannin B1 could be one of the Annals of Medicine & Healthcare Research Page 452 potential lead drug compound in the treatment of type 2 diabetes.
author2	Nejati, Mostafa
author_facet	Nejati, Mostafa Bakhtiar, M. Taher Abdul Majid, Fadzilah Adibah Sarmidi, Mohamad Roji
format	Book Chapter
author	Bakhtiar, M. Taher Abdul Majid, Fadzilah Adibah Sarmidi, Mohamad Roji
author_sort	Bakhtiar, M. Taher
title	Cinnamtannin B1: a small compound having antidiabetes properties
title_short	Cinnamtannin B1: a small compound having antidiabetes properties
title_full	Cinnamtannin B1: a small compound having antidiabetes properties
title_fullStr	Cinnamtannin B1: a small compound having antidiabetes properties
title_full_unstemmed	Cinnamtannin B1: a small compound having antidiabetes properties
title_sort	cinnamtannin b1: a small compound having antidiabetes properties
publisher	Universal Publisher
publishDate	2009
url	http://irep.iium.edu.my/6474/ http://irep.iium.edu.my/6474/1/ebook_iomc.pdf
first_indexed	2023-09-18T20:15:26Z
last_indexed	2023-09-18T20:15:26Z
_version_	1777407755822301184

Cinnamtannin B1: a small compound having antidiabetes properties

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