Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin

Background This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity. Methods Fifty‐four heat cured rectangular DBR specimens (64 x 10 x 3.3 ± 0.2 mm) containing nine concentr...

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Main Authors: Steve, An, Judge, Roy B, Wong, Rebecca H, Arzmi, Mohd Hafiz, Palamara, J E, Dashper, Stuart
Format: Article
Language:English
English
English
Published: Wiley Online Library 2018
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Online Access:http://irep.iium.edu.my/64442/
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http://irep.iium.edu.my/64442/
http://irep.iium.edu.my/64442/7/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_SCOPUS.pdf
http://irep.iium.edu.my/64442/19/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial.pdf
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spelling iium-644422019-01-31T06:38:10Z http://irep.iium.edu.my/64442/ Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin Steve, An Judge, Roy B Wong, Rebecca H Arzmi, Mohd Hafiz Palamara, J E Dashper, Stuart QR Microbiology RK Dentistry Background This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity. Methods Fifty‐four heat cured rectangular DBR specimens (64 x 10 x 3.3 ± 0.2 mm) containing nine concentrations of PTMC between 0 ‐ 5% (wt/wt) were fabricated and subjected to a three‐point bending test. A phytoncide release bioassay was developed using DBR containing 0% and 2.5% PTMCs (wt/wt) in a 24 well‐plate assay with incubation of Porphyromonas gingivalis at 37°C for 74 h. The antifungal activity of PTMCs against Candida albicans, in a pH 5.5 acidic environment was determined in a plate assay. Results Flexural strength decreased with increasing PTMC concentration from 97.58 ± 4.79 MPa for the DBR alone to 53.66 ± 2.46 MPa for DBR containing 5.0% PTMC. No release of phytoncide from the PTMCs in the DBR was detected at pH 7.4. The PTMCs had a minimal inhibitory concentration of 2.6% (wt/vol) against C. albicans at pH 5.5. Conclusions PTMCs can be added to DBR 2.5% (wt/wt) without adversely affecting flexural strength. PTMCs released the antimicrobial agent at pH 5.5 at concentrations sufficient to inhibit the growth of the C. albicans. Wiley Online Library 2018-06 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/64442/7/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/64442/19/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial.pdf application/pdf en http://irep.iium.edu.my/64442/20/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_WOS.pdf Steve, An and Judge, Roy B and Wong, Rebecca H and Arzmi, Mohd Hafiz and Palamara, J E and Dashper, Stuart (2018) Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin. Australian Dental Journal, 63 (3). pp. 302-311. ISSN 1834-7819 https://onlinelibrary.wiley.com/doi/pdf/10.1111/adj.12640 10.1111/adj.12640
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
topic QR Microbiology
RK Dentistry
spellingShingle QR Microbiology
RK Dentistry
Steve, An
Judge, Roy B
Wong, Rebecca H
Arzmi, Mohd Hafiz
Palamara, J E
Dashper, Stuart
Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
description Background This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity. Methods Fifty‐four heat cured rectangular DBR specimens (64 x 10 x 3.3 ± 0.2 mm) containing nine concentrations of PTMC between 0 ‐ 5% (wt/wt) were fabricated and subjected to a three‐point bending test. A phytoncide release bioassay was developed using DBR containing 0% and 2.5% PTMCs (wt/wt) in a 24 well‐plate assay with incubation of Porphyromonas gingivalis at 37°C for 74 h. The antifungal activity of PTMCs against Candida albicans, in a pH 5.5 acidic environment was determined in a plate assay. Results Flexural strength decreased with increasing PTMC concentration from 97.58 ± 4.79 MPa for the DBR alone to 53.66 ± 2.46 MPa for DBR containing 5.0% PTMC. No release of phytoncide from the PTMCs in the DBR was detected at pH 7.4. The PTMCs had a minimal inhibitory concentration of 2.6% (wt/vol) against C. albicans at pH 5.5. Conclusions PTMCs can be added to DBR 2.5% (wt/wt) without adversely affecting flexural strength. PTMCs released the antimicrobial agent at pH 5.5 at concentrations sufficient to inhibit the growth of the C. albicans.
format Article
author Steve, An
Judge, Roy B
Wong, Rebecca H
Arzmi, Mohd Hafiz
Palamara, J E
Dashper, Stuart
author_facet Steve, An
Judge, Roy B
Wong, Rebecca H
Arzmi, Mohd Hafiz
Palamara, J E
Dashper, Stuart
author_sort Steve, An
title Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_short Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_full Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_fullStr Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_full_unstemmed Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_sort incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
publisher Wiley Online Library
publishDate 2018
url http://irep.iium.edu.my/64442/
http://irep.iium.edu.my/64442/
http://irep.iium.edu.my/64442/
http://irep.iium.edu.my/64442/7/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_SCOPUS.pdf
http://irep.iium.edu.my/64442/19/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial.pdf
http://irep.iium.edu.my/64442/20/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_WOS.pdf
first_indexed 2023-09-18T21:31:27Z
last_indexed 2023-09-18T21:31:27Z
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