Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
Background This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity. Methods Fifty‐four heat cured rectangular DBR specimens (64 x 10 x 3.3 ± 0.2 mm) containing nine concentr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Wiley Online Library
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/64442/ http://irep.iium.edu.my/64442/ http://irep.iium.edu.my/64442/ http://irep.iium.edu.my/64442/7/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_SCOPUS.pdf http://irep.iium.edu.my/64442/19/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial.pdf http://irep.iium.edu.my/64442/20/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_WOS.pdf |
| Summary: | Background
This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity.
Methods
Fifty‐four heat cured rectangular DBR specimens (64 x 10 x 3.3 ± 0.2 mm) containing nine concentrations of PTMC between 0 ‐ 5% (wt/wt) were fabricated and subjected to a three‐point bending test. A phytoncide release bioassay was developed using DBR containing 0% and 2.5% PTMCs (wt/wt) in a 24 well‐plate assay with incubation of Porphyromonas gingivalis at 37°C for 74 h. The antifungal activity of PTMCs against Candida albicans, in a pH 5.5 acidic environment was determined in a plate assay.
Results
Flexural strength decreased with increasing PTMC concentration from 97.58 ± 4.79 MPa for the DBR alone to 53.66 ± 2.46 MPa for DBR containing 5.0% PTMC. No release of phytoncide from the PTMCs in the DBR was detected at pH 7.4. The PTMCs had a minimal inhibitory concentration of 2.6% (wt/vol) against C. albicans at pH 5.5.
Conclusions
PTMCs can be added to DBR 2.5% (wt/wt) without adversely affecting flexural strength. PTMCs released the antimicrobial agent at pH 5.5 at concentrations sufficient to inhibit the growth of the C. albicans. |
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