Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil

In the current study, 5-FU-loaded nanoparticles (NPs) were prepared using polylactic-co-glycolic acid (PLGA), polycaprolactone (PCL), di-block poly lactide-co-caprolactone (PLCL) and tri-block poly L-lactide-co-caprolactone-co-glycolide (PLCLG). The influence of these polymers on the particle sizes,...

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Main Authors:	Ashour, Abdelkader Elbadawy Abbas, Badran, Mohamed M., Kumar, Ashok, Rishi, Arun K., Yassin, Alaa Eldeen Bakry
Format:	Article
Language:	English
Published:	IEEE 2016
Subjects:	RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/64277/ http://irep.iium.edu.my/64277/ http://irep.iium.edu.my/64277/ http://irep.iium.edu.my/64277/1/Di-Block%20PLCL%20and%20Tri-Block%20PLCLG%20Matrix%20Polymeric%20Nanoparticles%20Enhanced%20the%20Anticancer%20Activity%20of%20Loaded%205-Fluorouracil.pdf

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spelling	iium-642772018-07-03T01:34:59Z http://irep.iium.edu.my/64277/ Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil Ashour, Abdelkader Elbadawy Abbas Badran, Mohamed M. Kumar, Ashok Rishi, Arun K. Yassin, Alaa Eldeen Bakry RM300 Drugs and their action In the current study, 5-FU-loaded nanoparticles (NPs) were prepared using polylactic-co-glycolic acid (PLGA), polycaprolactone (PCL), di-block poly lactide-co-caprolactone (PLCL) and tri-block poly L-lactide-co-caprolactone-co-glycolide (PLCLG). The influence of these polymers on the particle sizes, morphology, drug loading, and in vitro drug release was investigated. The anticancer activity was assessed utilizing MTT assay in three human cancer cell lines of different tissue origin; brain (Daoy), liver (HepG2), and colorectal (HT29) using suitable negative and positive controls. The prepared NPs showed a uniform spherical shape with an average size range of 193.5± 6.3 to 303.5± 3.3 nm with negative zeta potential. The entrapment efficiency achieved with F4-F6 (block copolymer NPs) was 78-79% and significantly higher compared with F1 PLGA (31%) and F2; PCL (37%). An initial rapid 5-FU release followed by a slow release ranging from 35% to 81% after 72 h was observed. All the prepared NPs formulations showed enhancement in the cytotoxicity of 5-FU towards all the three cancer cell lines. Generally, block copolymer NPs (F4-F6) showed higher % cell death over PLGA (F1) and PCL (F2) NPs after 48 and 72 h incubation in the case of HepG2 and HT-29. The incorporation of PEG with the tri-block (F6) caused a significant increase in the cytotoxicity of NPs in all of the three cancer cell lines. Block copolymer-based NPs can be considered as promising carriers for enhancing the efficacy of 5-FU in cancer therapy. IEEE 2016-09-27 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64277/1/Di-Block%20PLCL%20and%20Tri-Block%20PLCLG%20Matrix%20Polymeric%20Nanoparticles%20Enhanced%20the%20Anticancer%20Activity%20of%20Loaded%205-Fluorouracil.pdf Ashour, Abdelkader Elbadawy Abbas and Badran, Mohamed M. and Kumar, Ashok and Rishi, Arun K. and Yassin, Alaa Eldeen Bakry (2016) Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil. IEEE Transactions on NanoBioscience, 15 (7). pp. 739-747. ISSN 1536-1241 E-ISSN 1558-2639 https://ieeexplore.ieee.org/document/7577830/ DOI: 10.1109/TNB.2016.2612340
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM300 Drugs and their action
spellingShingle	RM300 Drugs and their action Ashour, Abdelkader Elbadawy Abbas Badran, Mohamed M. Kumar, Ashok Rishi, Arun K. Yassin, Alaa Eldeen Bakry Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil
description	In the current study, 5-FU-loaded nanoparticles (NPs) were prepared using polylactic-co-glycolic acid (PLGA), polycaprolactone (PCL), di-block poly lactide-co-caprolactone (PLCL) and tri-block poly L-lactide-co-caprolactone-co-glycolide (PLCLG). The influence of these polymers on the particle sizes, morphology, drug loading, and in vitro drug release was investigated. The anticancer activity was assessed utilizing MTT assay in three human cancer cell lines of different tissue origin; brain (Daoy), liver (HepG2), and colorectal (HT29) using suitable negative and positive controls. The prepared NPs showed a uniform spherical shape with an average size range of 193.5± 6.3 to 303.5± 3.3 nm with negative zeta potential. The entrapment efficiency achieved with F4-F6 (block copolymer NPs) was 78-79% and significantly higher compared with F1 PLGA (31%) and F2; PCL (37%). An initial rapid 5-FU release followed by a slow release ranging from 35% to 81% after 72 h was observed. All the prepared NPs formulations showed enhancement in the cytotoxicity of 5-FU towards all the three cancer cell lines. Generally, block copolymer NPs (F4-F6) showed higher % cell death over PLGA (F1) and PCL (F2) NPs after 48 and 72 h incubation in the case of HepG2 and HT-29. The incorporation of PEG with the tri-block (F6) caused a significant increase in the cytotoxicity of NPs in all of the three cancer cell lines. Block copolymer-based NPs can be considered as promising carriers for enhancing the efficacy of 5-FU in cancer therapy.
format	Article
author	Ashour, Abdelkader Elbadawy Abbas Badran, Mohamed M. Kumar, Ashok Rishi, Arun K. Yassin, Alaa Eldeen Bakry
author_facet	Ashour, Abdelkader Elbadawy Abbas Badran, Mohamed M. Kumar, Ashok Rishi, Arun K. Yassin, Alaa Eldeen Bakry
author_sort	Ashour, Abdelkader Elbadawy Abbas
title	Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil
title_short	Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil
title_full	Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil
title_fullStr	Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil
title_full_unstemmed	Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil
title_sort	di-block plcl and tri-block plclg matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil
publisher	IEEE
publishDate	2016
url	http://irep.iium.edu.my/64277/ http://irep.iium.edu.my/64277/ http://irep.iium.edu.my/64277/ http://irep.iium.edu.my/64277/1/Di-Block%20PLCL%20and%20Tri-Block%20PLCLG%20Matrix%20Polymeric%20Nanoparticles%20Enhanced%20the%20Anticancer%20Activity%20of%20Loaded%205-Fluorouracil.pdf
first_indexed	2023-09-18T21:31:11Z
last_indexed	2023-09-18T21:31:11Z
_version_	1777412522065788928

Di-Block PLCL and Tri-Block PLCLG matrix polymeric nanoparticles enhanced the anticancer activity of loaded 5-fluorouracil

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