Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes

Metallic silver (Ag) and some Ag compounds have long being used to fight microbial infection even before the emergence of antibiotics in the form of drugs. But lately, one of the problems facing the medical world is the occurrence of drug resistance microbes. Therefore, actions must be taken to o...

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Main Authors:	Yusof, Faridah, Chowdhury, Silvia, Sulaiman, Nadzril, Faruck, Mohammad Omer
Format:	Article
Language:	English English
Published:	UTM Press 2018
Subjects:	QD Chemistry QR Microbiology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/64232/ http://irep.iium.edu.my/64232/ http://irep.iium.edu.my/64232/8/64232%20Effect%20of%20process%20parameters%20on%20the%20synthesis.pdf http://irep.iium.edu.my/64232/7/64232%20Effect%20of%20process%20parameters%20on%20the%20synthesis%20SCOPUS.pdf

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spelling	iium-642322019-03-13T07:23:31Z http://irep.iium.edu.my/64232/ Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes Yusof, Faridah Chowdhury, Silvia Sulaiman, Nadzril Faruck, Mohammad Omer QD Chemistry QR Microbiology TP248.13 Biotechnology Metallic silver (Ag) and some Ag compounds have long being used to fight microbial infection even before the emergence of antibiotics in the form of drugs. But lately, one of the problems facing the medical world is the occurrence of drug resistance microbes. Therefore, actions must be taken to overcome the problem, one of which is to continue studies to develop new drugs, either synthetic or natural. The ultimate goal is to offer appropriate and efficient antimicrobial drugs to patients. In this regard, with the advent of nanotechnology, it is now possible to synthesize nano scale Ag which can be used in diverse medical applications. The aim of this study is to investigate the effect of some important parameters towards the production of high yield Ag nanoparticles (AgNPs) from silver salts by making use of reducing agent. One-Factor-at-a-Time (OFAT) design of experiment was conducted, involving five important synthesis parameters. Based on the analyses of its yellow color, absorption by UV-vis in the range of 350 to 420 nm wavelength and surface plasmon resonance (SPR) at 420 nm, AgNPs produced were estimated to be in the range of 5 to 50 nm in diameter. Out of the five parameters tested, such as the concentration of silver nitrate (AgNO3), the initial pH of AgNO3 solution, the concentration of tri-sodium citrate (TSC), the reaction time between AgNO3 and TSC and stirring time (after reduction process), results showed that all factors except stirring time affect the production. Characterization of AgNPs, carried out using Transmission Electron Microscope (TEM), showed that the synthesized AgNPs were spherical with diameters less than 15 nm. Antimicrobial activity assays of AgNPs, carried out against selected microbes, namely Aspergillus niger, Microsporum canis and Staphylococcus aureus showed various degree of potencies. UTM Press 2018-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64232/8/64232%20Effect%20of%20process%20parameters%20on%20the%20synthesis.pdf application/pdf en http://irep.iium.edu.my/64232/7/64232%20Effect%20of%20process%20parameters%20on%20the%20synthesis%20SCOPUS.pdf Yusof, Faridah and Chowdhury, Silvia and Sulaiman, Nadzril and Faruck, Mohammad Omer (2018) Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes. Jurnal Teknologi, 80 (3). pp. 115-121. E-ISSN 2180-3722. https://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/11465/6366
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry QR Microbiology TP248.13 Biotechnology
spellingShingle	QD Chemistry QR Microbiology TP248.13 Biotechnology Yusof, Faridah Chowdhury, Silvia Sulaiman, Nadzril Faruck, Mohammad Omer Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes
description	Metallic silver (Ag) and some Ag compounds have long being used to fight microbial infection even before the emergence of antibiotics in the form of drugs. But lately, one of the problems facing the medical world is the occurrence of drug resistance microbes. Therefore, actions must be taken to overcome the problem, one of which is to continue studies to develop new drugs, either synthetic or natural. The ultimate goal is to offer appropriate and efficient antimicrobial drugs to patients. In this regard, with the advent of nanotechnology, it is now possible to synthesize nano scale Ag which can be used in diverse medical applications. The aim of this study is to investigate the effect of some important parameters towards the production of high yield Ag nanoparticles (AgNPs) from silver salts by making use of reducing agent. One-Factor-at-a-Time (OFAT) design of experiment was conducted, involving five important synthesis parameters. Based on the analyses of its yellow color, absorption by UV-vis in the range of 350 to 420 nm wavelength and surface plasmon resonance (SPR) at 420 nm, AgNPs produced were estimated to be in the range of 5 to 50 nm in diameter. Out of the five parameters tested, such as the concentration of silver nitrate (AgNO3), the initial pH of AgNO3 solution, the concentration of tri-sodium citrate (TSC), the reaction time between AgNO3 and TSC and stirring time (after reduction process), results showed that all factors except stirring time affect the production. Characterization of AgNPs, carried out using Transmission Electron Microscope (TEM), showed that the synthesized AgNPs were spherical with diameters less than 15 nm. Antimicrobial activity assays of AgNPs, carried out against selected microbes, namely Aspergillus niger, Microsporum canis and Staphylococcus aureus showed various degree of potencies.
format	Article
author	Yusof, Faridah Chowdhury, Silvia Sulaiman, Nadzril Faruck, Mohammad Omer
author_facet	Yusof, Faridah Chowdhury, Silvia Sulaiman, Nadzril Faruck, Mohammad Omer
author_sort	Yusof, Faridah
title	Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes
title_short	Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes
title_full	Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes
title_fullStr	Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes
title_full_unstemmed	Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes
title_sort	effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes
publisher	UTM Press
publishDate	2018
url	http://irep.iium.edu.my/64232/ http://irep.iium.edu.my/64232/ http://irep.iium.edu.my/64232/8/64232%20Effect%20of%20process%20parameters%20on%20the%20synthesis.pdf http://irep.iium.edu.my/64232/7/64232%20Effect%20of%20process%20parameters%20on%20the%20synthesis%20SCOPUS.pdf
first_indexed	2023-09-18T21:31:07Z
last_indexed	2023-09-18T21:31:07Z
_version_	1777412517122801664

Effect of process parameters on the synthesis of silver nanoparticles and its effects on microbes

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