Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation
INTRODUCTION: Treatment of osteomyelitis is still a major challenge in orthopaedic field. New Zealand White rabbit is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective...
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Malaysian Orthopaedic Association (MOA)
2017
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| Online Access: | http://irep.iium.edu.my/64068/ http://irep.iium.edu.my/64068/ http://irep.iium.edu.my/64068/3/PR01C-Treatment-Osteomyelitis-New-Zealand.pdf |
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iium-640682018-07-23T02:43:00Z http://irep.iium.edu.my/64068/ Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Aziz, Aziah RD701 Orthopedics INTRODUCTION: Treatment of osteomyelitis is still a major challenge in orthopaedic field. New Zealand White rabbit is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of the study is to evaluate the outcome treatment of osteomyelitis in rabbit femur with impregnated Hydroxyappatite Gentamicin. Evaluation at stipulated durations using microCT Skyscan 1176 system. MATERIALS & METHODS: Osteomyelitis is created by inoculation of Staphylococcus aureus ATCC 25923 into rabbit’s distal femur. The rabbit were divided with 3, 6, 12, 26 week assessments. The response of the biomaterials (hydroxyapatite) impregnated with gentamicin against the infection were evaluated through microcomputed topography using Skyscan 1176 system.The bone analysis data were obtained by the software provided by Skyscan 1176 system. The distal femoral shaft were scanned using A1 1mm filter with 18 µm pixel resolutions. Bacterial study of culture and sensitivity, coagulase test, catalase test and gram stain were done to determine the treatment is effective. RESULTS: The data of bone analysis were for tissue volume (TV), bone surface (BS), bone volume (BV), and trabecular thickness (Tb.Th) in all groups were illustrated at Table 1. The bone healing noted in the treated group. The results showed no bacterial were grow in the agar and all test were negative at 26 weeks interval. Table 1 showing the bone analysis through Skyscan1176 system. DISCUSSIONS: Osteomyelitic changes were noted in all rabbit after inoculation of bacteria at week three and six. The micro-computed topographic analysis at 3, 6, 12 and 26 weeks of interval, showed increase bone parameters in treated femur. The bone volume (BV) was increased with value 124.96 mm3 compared to sham group (no treatment given) with bone volume 66.96 mm3. The density of biomaterials was decreased with duration of studies. This data showed that micro-computed topography studies is capable to provide information of bone microstructures in rapid and non-destructive way. The findings showed that impregnated biomaterials with gentamicin have potential to be used in osteomyelitis infection. CONCLUSION: Computed topography results showed at 26 weeks of treatment, the impregnated biomaterials have a potential in treatment of osteomyelitis. Malaysian Orthopaedic Association (MOA) 2017-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64068/3/PR01C-Treatment-Osteomyelitis-New-Zealand.pdf Zulkifly, Ahmad Hafiz and Mohd Jan, Nurul Hafiza and Ibrahim, Mohd Zulfadzli and Aziz, Aziah (2017) Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation. Malaysian Orthopaedic Journal, 11 (Supplement A). PR01C-PR01C. ISSN 1985-2533 E-ISSN 2232-111X http://www.morthoj.org/supplements/moa/PR01C-Treatment-Osteomyelitis-New-Zealand.pdf |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
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IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
RD701 Orthopedics |
| spellingShingle |
RD701 Orthopedics Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Aziz, Aziah Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation |
| description |
INTRODUCTION:
Treatment of osteomyelitis is still a major
challenge in orthopaedic field. New Zealand
White rabbit is acceptable experimental model
described to develop effective local delivery of
antibiotics osteomyelitis treatment as it can
mimics the disease process in human. The
objective of the study is to evaluate the
outcome treatment of osteomyelitis in rabbit
femur with impregnated Hydroxyappatite
Gentamicin. Evaluation at stipulated durations
using microCT Skyscan 1176 system.
MATERIALS & METHODS:
Osteomyelitis is created by inoculation of
Staphylococcus aureus ATCC 25923 into
rabbit’s distal femur. The rabbit were divided
with 3, 6, 12, 26 week assessments. The
response of the biomaterials (hydroxyapatite)
impregnated with gentamicin against the
infection were evaluated through microcomputed
topography using Skyscan 1176
system.The bone analysis data were obtained
by the software provided by Skyscan 1176
system. The distal femoral shaft were scanned
using A1 1mm filter with 18 µm pixel
resolutions. Bacterial study of culture and
sensitivity, coagulase test, catalase test and
gram stain were done to determine the
treatment is effective.
RESULTS:
The data of bone analysis were for tissue
volume (TV), bone surface (BS), bone volume
(BV), and trabecular thickness (Tb.Th) in all
groups were illustrated at Table 1. The bone
healing noted in the treated group. The results
showed no bacterial were grow in the agar and
all test were negative at 26 weeks interval.
Table 1 showing the bone analysis through
Skyscan1176 system.
DISCUSSIONS:
Osteomyelitic changes were noted in all rabbit
after inoculation of bacteria at week three and
six. The micro-computed topographic analysis
at 3, 6, 12 and 26 weeks of interval, showed
increase bone parameters in treated femur. The
bone volume (BV) was increased with value
124.96 mm3
compared to sham group (no
treatment given) with bone volume 66.96 mm3. The density of biomaterials was decreased with
duration of studies. This data showed that
micro-computed topography studies is capable
to provide information of bone microstructures
in rapid and non-destructive way. The findings
showed that impregnated biomaterials with
gentamicin have potential to be used in
osteomyelitis infection.
CONCLUSION:
Computed topography results showed at 26
weeks of treatment, the impregnated
biomaterials have a potential in treatment of
osteomyelitis. |
| format |
Article |
| author |
Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Aziz, Aziah |
| author_facet |
Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Aziz, Aziah |
| author_sort |
Zulkifly, Ahmad Hafiz |
| title |
Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation |
| title_short |
Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation |
| title_full |
Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation |
| title_fullStr |
Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation |
| title_full_unstemmed |
Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation |
| title_sort |
treatment of osteomyelitis in new zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation |
| publisher |
Malaysian Orthopaedic Association (MOA) |
| publishDate |
2017 |
| url |
http://irep.iium.edu.my/64068/ http://irep.iium.edu.my/64068/ http://irep.iium.edu.my/64068/3/PR01C-Treatment-Osteomyelitis-New-Zealand.pdf |
| first_indexed |
2023-09-18T21:30:52Z |
| last_indexed |
2023-09-18T21:30:52Z |
| _version_ |
1777412501661548544 |