A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS

Background: Volatile organic compounds (VOCs) emitted from exhaled breath from human bodies have been proven to be a useful source of information for early lung cancer diagnosis. To date, there are still arguable information on the production and origin of significant VOCs of cancer cells. Thus, thi...

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Main Authors:	Thriumani, Reena, Zakaria, Ammar, Hashim, Yumi Zuhanis Has-Yun, Jeffree, Amanina Iymia, Helmy, Khaled Mohamed, Kamarudin, Latifah Munirah, Omar, Mohammad Iqbal, Md Shakaff, Ali Yeon, Adom, Abdul Hamid, Persaud, Krishna C.
Format:	Article
Language:	English English English
Published:	BioMed Central Ltd. 2018
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/63915/ http://irep.iium.edu.my/63915/ http://irep.iium.edu.my/63915/ http://irep.iium.edu.my/63915/1/63915_A%20study%20on%20volatile%20organic%20compounds_article.pdf http://irep.iium.edu.my/63915/2/63915_A%20study%20on%20volatile%20organic%20compounds_scopus.pdf http://irep.iium.edu.my/63915/13/63915_A%20study%20on%20volatile%20organic%20compounds_WoS.pdf

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spelling	iium-639152019-01-24T01:40:37Z http://irep.iium.edu.my/63915/ A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS Thriumani, Reena Zakaria, Ammar Hashim, Yumi Zuhanis Has-Yun Jeffree, Amanina Iymia Helmy, Khaled Mohamed Kamarudin, Latifah Munirah Omar, Mohammad Iqbal Md Shakaff, Ali Yeon Adom, Abdul Hamid Persaud, Krishna C. T Technology (General) Background: Volatile organic compounds (VOCs) emitted from exhaled breath from human bodies have been proven to be a useful source of information for early lung cancer diagnosis. To date, there are still arguable information on the production and origin of significant VOCs of cancer cells. Thus, this study aims to conduct in-vitro experiments involving related cell lines to verify the capability of VOCs in providing information of the cells. Method: The performances of e-nose technology with different statistical methods to determine the best classifier were conducted and discussed. The gas sensor study has been complemented using solid phase micro-extraction-gas chromatography mass spectrometry. For this purpose, the lung cancer cells (A549 and Calu-3) and control cell lines, breast cancer cell (MCF7) and non-cancerous lung cell (WI38VA13) were cultured in growth medium. Results: This study successfully provided a list of possible volatile organic compounds that can be specific biomarkers for lung cancer, even at the 24th hour of cell growth. Also, the Linear Discriminant Analysis-based One versus All-Support Vector Machine classifier, is able to produce high performance in distinguishing lung cancer from breast cancer cells and normal lung cells. Conclusion: The findings in this work conclude that the specific VOC released from the cancer cells can act as the odour signature and potentially to be used as non-invasive screening of lung cancer using gas array sensor devices. BioMed Central Ltd. 2018-04-02 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/63915/1/63915_A%20study%20on%20volatile%20organic%20compounds_article.pdf application/pdf en http://irep.iium.edu.my/63915/2/63915_A%20study%20on%20volatile%20organic%20compounds_scopus.pdf application/pdf en http://irep.iium.edu.my/63915/13/63915_A%20study%20on%20volatile%20organic%20compounds_WoS.pdf Thriumani, Reena and Zakaria, Ammar and Hashim, Yumi Zuhanis Has-Yun and Jeffree, Amanina Iymia and Helmy, Khaled Mohamed and Kamarudin, Latifah Munirah and Omar, Mohammad Iqbal and Md Shakaff, Ali Yeon and Adom, Abdul Hamid and Persaud, Krishna C. (2018) A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS. BMC Cancer, 18 (1). pp. 1-17. ISSN 1471-2407 https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-018-4235-7 10.1186/s12885-018-4235-7
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	T Technology (General)
spellingShingle	T Technology (General) Thriumani, Reena Zakaria, Ammar Hashim, Yumi Zuhanis Has-Yun Jeffree, Amanina Iymia Helmy, Khaled Mohamed Kamarudin, Latifah Munirah Omar, Mohammad Iqbal Md Shakaff, Ali Yeon Adom, Abdul Hamid Persaud, Krishna C. A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS
description	Background: Volatile organic compounds (VOCs) emitted from exhaled breath from human bodies have been proven to be a useful source of information for early lung cancer diagnosis. To date, there are still arguable information on the production and origin of significant VOCs of cancer cells. Thus, this study aims to conduct in-vitro experiments involving related cell lines to verify the capability of VOCs in providing information of the cells. Method: The performances of e-nose technology with different statistical methods to determine the best classifier were conducted and discussed. The gas sensor study has been complemented using solid phase micro-extraction-gas chromatography mass spectrometry. For this purpose, the lung cancer cells (A549 and Calu-3) and control cell lines, breast cancer cell (MCF7) and non-cancerous lung cell (WI38VA13) were cultured in growth medium. Results: This study successfully provided a list of possible volatile organic compounds that can be specific biomarkers for lung cancer, even at the 24th hour of cell growth. Also, the Linear Discriminant Analysis-based One versus All-Support Vector Machine classifier, is able to produce high performance in distinguishing lung cancer from breast cancer cells and normal lung cells. Conclusion: The findings in this work conclude that the specific VOC released from the cancer cells can act as the odour signature and potentially to be used as non-invasive screening of lung cancer using gas array sensor devices.
format	Article
author	Thriumani, Reena Zakaria, Ammar Hashim, Yumi Zuhanis Has-Yun Jeffree, Amanina Iymia Helmy, Khaled Mohamed Kamarudin, Latifah Munirah Omar, Mohammad Iqbal Md Shakaff, Ali Yeon Adom, Abdul Hamid Persaud, Krishna C.
author_facet	Thriumani, Reena Zakaria, Ammar Hashim, Yumi Zuhanis Has-Yun Jeffree, Amanina Iymia Helmy, Khaled Mohamed Kamarudin, Latifah Munirah Omar, Mohammad Iqbal Md Shakaff, Ali Yeon Adom, Abdul Hamid Persaud, Krishna C.
author_sort	Thriumani, Reena
title	A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS
title_short	A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS
title_full	A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS
title_fullStr	A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS
title_full_unstemmed	A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS
title_sort	study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and spme-gcms
publisher	BioMed Central Ltd.
publishDate	2018
url	http://irep.iium.edu.my/63915/ http://irep.iium.edu.my/63915/ http://irep.iium.edu.my/63915/ http://irep.iium.edu.my/63915/1/63915_A%20study%20on%20volatile%20organic%20compounds_article.pdf http://irep.iium.edu.my/63915/2/63915_A%20study%20on%20volatile%20organic%20compounds_scopus.pdf http://irep.iium.edu.my/63915/13/63915_A%20study%20on%20volatile%20organic%20compounds_WoS.pdf
first_indexed	2023-09-18T21:30:39Z
last_indexed	2023-09-18T21:30:39Z
_version_	1777412488031109120

A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPME-GCMS

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