Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employ...

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Main Authors:	Yusan, Sabriye, M. Rahman, Mokhlesur, Mohamad, Nasir A.N., ,, Tengku M. Arrif, Abdul Latif, Ahmad Zubaidi, Md Aris, Mohd Aznan, Wan Nik, Wan Sani
Format:	Article
Language:	English English English
Published:	Hindawi 2018
Subjects:	R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica RZ Other systems of medicine
Online Access:	http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf http://irep.iium.edu.my/63788/2/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_SCOPUS.pdf http://irep.iium.edu.my/63788/3/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_WoS.pdf

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spelling	iium-637882018-07-30T02:23:14Z http://irep.iium.edu.my/63788/ Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite Yusan, Sabriye M. Rahman, Mokhlesur Mohamad, Nasir A.N. ,, Tengku M. Arrif Abdul Latif, Ahmad Zubaidi Md Aris, Mohd Aznan Wan Nik, Wan Sani R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica RZ Other systems of medicine A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibrationplotisobtainedoverawideconcentrationrangeofglucosefrom1×10−5 to2×10−3M.Underoptimalconditions,the biosensor exhibits high sensitivity (0.34µA·mM−1), low detection limit (1×10−4M), high aﬃnity to glucose (Km�0.02mM), and also good reproducibility (R.S.D. 2.8%, n � 10) and a stability of about ten days when stored dry at +4°C. Besides, the eﬀectsofpHvalue,scanrate,mediatoreﬀectsontheglucosecurrent,andelectroactiveinterferenceofthebiosensorwerealso discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility. Hindawi 2018-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf application/pdf en http://irep.iium.edu.my/63788/2/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/63788/3/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_WoS.pdf Yusan, Sabriye and M. Rahman, Mokhlesur and Mohamad, Nasir A.N. and ,, Tengku M. Arrif and Abdul Latif, Ahmad Zubaidi and Md Aris, Mohd Aznan and Wan Nik, Wan Sani (2018) Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite. Journal of Analytical Methods in Chemistry, 2018. pp. 1-8. ISSN 2090-8865 E-ISSN 2090-8873 https://doi.org/10.1155/2018/2687341 10.1155/2018/2687341
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica RZ Other systems of medicine
spellingShingle	R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica RZ Other systems of medicine Yusan, Sabriye M. Rahman, Mokhlesur Mohamad, Nasir A.N. ,, Tengku M. Arrif Abdul Latif, Ahmad Zubaidi Md Aris, Mohd Aznan Wan Nik, Wan Sani Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
description	A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibrationplotisobtainedoverawideconcentrationrangeofglucosefrom1×10−5 to2×10−3M.Underoptimalconditions,the biosensor exhibits high sensitivity (0.34µA·mM−1), low detection limit (1×10−4M), high aﬃnity to glucose (Km�0.02mM), and also good reproducibility (R.S.D. 2.8%, n � 10) and a stability of about ten days when stored dry at +4°C. Besides, the eﬀectsofpHvalue,scanrate,mediatoreﬀectsontheglucosecurrent,andelectroactiveinterferenceofthebiosensorwerealso discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.
format	Article
author	Yusan, Sabriye M. Rahman, Mokhlesur Mohamad, Nasir A.N. ,, Tengku M. Arrif Abdul Latif, Ahmad Zubaidi Md Aris, Mohd Aznan Wan Nik, Wan Sani
author_facet	Yusan, Sabriye M. Rahman, Mokhlesur Mohamad, Nasir A.N. ,, Tengku M. Arrif Abdul Latif, Ahmad Zubaidi Md Aris, Mohd Aznan Wan Nik, Wan Sani
author_sort	Yusan, Sabriye
title	Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_short	Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_full	Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_fullStr	Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_full_unstemmed	Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_sort	development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the se-mcm-41 mesoporous composite
publisher	Hindawi
publishDate	2018
url	http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf http://irep.iium.edu.my/63788/2/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_SCOPUS.pdf http://irep.iium.edu.my/63788/3/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_WoS.pdf
first_indexed	2023-09-18T21:30:28Z
last_indexed	2023-09-18T21:30:28Z
_version_	1777412476939272192

Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite

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