The effect of chlorpyrifos on the aorta

Atherosclerosis is defined as a slow process of inflammation in medium-sized and large arteries fuelled by deposition of lipid. Classified as one of the major non communicable disease, it is accounted for the health burdens facing by many countries. Chlorpyrifos (CPF) is an organophosphorous insecti...

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Main Authors:	Abdul Razak, Redzuan Nul Hakim, Buyong, Zunariah, Abdullah, Nor Zamzila, A. Talib, Norlelawati
Format:	Book
Language:	English
Published:	IIUM Press, International Islamic University Malaysia 2017
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/63562/ http://irep.iium.edu.my/63562/ http://irep.iium.edu.my/63562/1/63562_The%20effect%20of%20chlorpyrifos%20on%20the%20aorta.pdf

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recordtype	eprints
spelling	iium-635622018-08-29T05:51:04Z http://irep.iium.edu.my/63562/ The effect of chlorpyrifos on the aorta Abdul Razak, Redzuan Nul Hakim Buyong, Zunariah Abdullah, Nor Zamzila A. Talib, Norlelawati R Medicine (General) Atherosclerosis is defined as a slow process of inflammation in medium-sized and large arteries fuelled by deposition of lipid. Classified as one of the major non communicable disease, it is accounted for the health burdens facing by many countries. Chlorpyrifos (CPF) is an organophosphorous insecticide that possesses various human systemic toxicities. The present study is therefore designed to demonstrate the atherosclerosis development in rats’ aorta following exposure to CPF. By using powerful tools such as scanning electron microscope and transmission electron microscope, we attempt to visualize ultrastructural alterations of intima layer covering advanced atherosclerotic plaque. Eighteen male Sprague–Dawley rats weighing 150-200 g were grouped into CPF exposed, vehicle exposed and control group. CPF exposed rats received subcutaneous injection of 18 mg/kg chlorpyrifos dissolved in vehicle with a volume of 0.7 ml/kg body weight while vehicle rats administered with vehicle containing 3% Dimethyl sulphoxide and 97% v/v soy oil. The rats were injected intermittently for 6 months. The rats were sacrificed at the end of 6 months of study and the aortic arch tissue was processed for electron microscopy. On observation, there were disruptions of the intimal layer of the CPF exposed rat aorta. Numerous notable endothelial gaps between endothelial cells were identified. The endothelial cells also were irregularly oriented and morphologically changed. Several areas of deendothelialization were noticed. There was no obvious ultrastructural change in the aorta of the rats fed with vehicle. As for transmission electron photomicrograph, foam cells and calcium crystals were found in CPF exposed rat aorta. No foam cell and calcium crystal were found in rats’ aorta exposed to vehicle and without treatment. Histological staining with toluidine blue able to identify several areas of intimal protrusions and elastic fibers disorientation in CPF exposed rat aorta. In conclusion, it is likely that prolonged CPF administration caused vascular wall damage and may be one of the mechanisms involved in CPF-induced atherosclerosis IIUM Press, International Islamic University Malaysia 2017-12 Book PeerReviewed application/pdf en http://irep.iium.edu.my/63562/1/63562_The%20effect%20of%20chlorpyrifos%20on%20the%20aorta.pdf Abdul Razak, Redzuan Nul Hakim and Buyong, Zunariah and Abdullah, Nor Zamzila and A. Talib, Norlelawati (2017) The effect of chlorpyrifos on the aorta. IIUM Press, International Islamic University Malaysia, Kuala Lumpur, Malaysia. ISBN 978-967-418-731-6 http://iiumpress.iium.edu.my/bookshop
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abdul Razak, Redzuan Nul Hakim Buyong, Zunariah Abdullah, Nor Zamzila A. Talib, Norlelawati The effect of chlorpyrifos on the aorta
description	Atherosclerosis is defined as a slow process of inflammation in medium-sized and large arteries fuelled by deposition of lipid. Classified as one of the major non communicable disease, it is accounted for the health burdens facing by many countries. Chlorpyrifos (CPF) is an organophosphorous insecticide that possesses various human systemic toxicities. The present study is therefore designed to demonstrate the atherosclerosis development in rats’ aorta following exposure to CPF. By using powerful tools such as scanning electron microscope and transmission electron microscope, we attempt to visualize ultrastructural alterations of intima layer covering advanced atherosclerotic plaque. Eighteen male Sprague–Dawley rats weighing 150-200 g were grouped into CPF exposed, vehicle exposed and control group. CPF exposed rats received subcutaneous injection of 18 mg/kg chlorpyrifos dissolved in vehicle with a volume of 0.7 ml/kg body weight while vehicle rats administered with vehicle containing 3% Dimethyl sulphoxide and 97% v/v soy oil. The rats were injected intermittently for 6 months. The rats were sacrificed at the end of 6 months of study and the aortic arch tissue was processed for electron microscopy. On observation, there were disruptions of the intimal layer of the CPF exposed rat aorta. Numerous notable endothelial gaps between endothelial cells were identified. The endothelial cells also were irregularly oriented and morphologically changed. Several areas of deendothelialization were noticed. There was no obvious ultrastructural change in the aorta of the rats fed with vehicle. As for transmission electron photomicrograph, foam cells and calcium crystals were found in CPF exposed rat aorta. No foam cell and calcium crystal were found in rats’ aorta exposed to vehicle and without treatment. Histological staining with toluidine blue able to identify several areas of intimal protrusions and elastic fibers disorientation in CPF exposed rat aorta. In conclusion, it is likely that prolonged CPF administration caused vascular wall damage and may be one of the mechanisms involved in CPF-induced atherosclerosis
format	Book
author	Abdul Razak, Redzuan Nul Hakim Buyong, Zunariah Abdullah, Nor Zamzila A. Talib, Norlelawati
author_facet	Abdul Razak, Redzuan Nul Hakim Buyong, Zunariah Abdullah, Nor Zamzila A. Talib, Norlelawati
author_sort	Abdul Razak, Redzuan Nul Hakim
title	The effect of chlorpyrifos on the aorta
title_short	The effect of chlorpyrifos on the aorta
title_full	The effect of chlorpyrifos on the aorta
title_fullStr	The effect of chlorpyrifos on the aorta
title_full_unstemmed	The effect of chlorpyrifos on the aorta
title_sort	effect of chlorpyrifos on the aorta
publisher	IIUM Press, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/63562/ http://irep.iium.edu.my/63562/ http://irep.iium.edu.my/63562/1/63562_The%20effect%20of%20chlorpyrifos%20on%20the%20aorta.pdf
first_indexed	2023-09-18T21:30:09Z
last_indexed	2023-09-18T21:30:09Z
_version_	1777412456613675008

The effect of chlorpyrifos on the aorta

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