Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia

CONTEXT: Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. OBJECTIVE: This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constitu...

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Main Authors:	Albadawi, Dina A., A. Mothana, Ramzi Ahmed, M. Khaled, Jamal, Ashour, Abdelkader Elbadawy Abbas, Kumar, Ashok, Ahmad, Sheikh Fayaz, Al-Said, Mansour Sulaiman, Al-Rehaily, Adnan Jathlan, M. Almusayeib, Nawal
Format:	Article
Language:	English English
Published:	Taylor and Francis Ltd. 2017
Subjects:	RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/63547/ http://irep.iium.edu.my/63547/ http://irep.iium.edu.my/63547/ http://irep.iium.edu.my/63547/1/Antimicrobial%2C%20anticancer%2C%20and%20antioxidant%20compounds%20from%20Premna%20resinosa%20growing%20in%20Saudi%20Arabia.pdf http://irep.iium.edu.my/63547/7/63547_Antimicrobial%2C%20anticancer%2C%20and%20antioxidant%20compounds_scopus.pdf

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recordtype	eprints
spelling	iium-635472018-05-15T02:08:27Z http://irep.iium.edu.my/63547/ Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia Albadawi, Dina A. A. Mothana, Ramzi Ahmed M. Khaled, Jamal Ashour, Abdelkader Elbadawy Abbas Kumar, Ashok Ahmad, Sheikh Fayaz Al-Said, Mansour Sulaiman Al-Rehaily, Adnan Jathlan M. Almusayeib, Nawal RM300 Drugs and their action CONTEXT: Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. OBJECTIVE: This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constituents. MATERIALS AND METHODS: The methanol extract of P. resinosa aerial parts and its fractions (n-hexane, dichloromethane, ethyl acetate and n-butanol) were tested. Antimicrobial activity was tested using microdilution method against three Gram-positive and four Gram-negative bacteria. The tested concentrations ranged from 4000 to 7.8 μg/mL and MIC values were determined after 24 h incubation. Anticancer activity was evaluated against three human cancer cell lines (Daoy, HepG2 and SK-MEL28) using MTT assay. Antioxidant activity was investigated by DPPH scavenging method and β-carotene-linoleic acid assay. RESULTS: The greatest antimicrobial activity was exhibited by n-hexane fraction (MIC 10 μg/mL) against Staphylococcus aureus, Enterococcus faecalis, and Shigella flexneri. The n-hexane fraction induced the greatest cytotoxic activity against Daoy, HepG2, and SK-MEL28 cell lines with IC50 values of 9.0, 8.5 and 13.2, respectively. Moreover, the dichloromethane and ethyl acetate fractions showed the highest antioxidant potential. A bioassay-guided fractionation led to the isolation and characterization of seven compounds for the first time, namely, quercetin (1), 3-methoxy quercetin (2), kaempferol (3), 3-methoxy kaempferol (4), myricetin 3,7,3'-trimethyl ether (5), lupeol (6), and stigmasterol (7). CONCLUSION: Our results indicate that P. resinosa is a source for antimicrobial and cytotoxic compounds. However, further work is required to isolate other active principles and to determine the mechanism of action. Taylor and Francis Ltd. 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63547/1/Antimicrobial%2C%20anticancer%2C%20and%20antioxidant%20compounds%20from%20Premna%20resinosa%20growing%20in%20Saudi%20Arabia.pdf application/pdf en http://irep.iium.edu.my/63547/7/63547_Antimicrobial%2C%20anticancer%2C%20and%20antioxidant%20compounds_scopus.pdf Albadawi, Dina A. and A. Mothana, Ramzi Ahmed and M. Khaled, Jamal and Ashour, Abdelkader Elbadawy Abbas and Kumar, Ashok and Ahmad, Sheikh Fayaz and Al-Said, Mansour Sulaiman and Al-Rehaily, Adnan Jathlan and M. Almusayeib, Nawal (2017) Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia. Pharmaceutical Biology, 55 (1). pp. 1759-1766. ISSN 1388-0209 E-ISSN 1744-5116 https://www.tandfonline.com/toc/iphb/current 10.1080/13880209.2017.1322617
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM300 Drugs and their action
spellingShingle	RM300 Drugs and their action Albadawi, Dina A. A. Mothana, Ramzi Ahmed M. Khaled, Jamal Ashour, Abdelkader Elbadawy Abbas Kumar, Ashok Ahmad, Sheikh Fayaz Al-Said, Mansour Sulaiman Al-Rehaily, Adnan Jathlan M. Almusayeib, Nawal Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia
description	CONTEXT: Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. OBJECTIVE: This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constituents. MATERIALS AND METHODS: The methanol extract of P. resinosa aerial parts and its fractions (n-hexane, dichloromethane, ethyl acetate and n-butanol) were tested. Antimicrobial activity was tested using microdilution method against three Gram-positive and four Gram-negative bacteria. The tested concentrations ranged from 4000 to 7.8 μg/mL and MIC values were determined after 24 h incubation. Anticancer activity was evaluated against three human cancer cell lines (Daoy, HepG2 and SK-MEL28) using MTT assay. Antioxidant activity was investigated by DPPH scavenging method and β-carotene-linoleic acid assay. RESULTS: The greatest antimicrobial activity was exhibited by n-hexane fraction (MIC 10 μg/mL) against Staphylococcus aureus, Enterococcus faecalis, and Shigella flexneri. The n-hexane fraction induced the greatest cytotoxic activity against Daoy, HepG2, and SK-MEL28 cell lines with IC50 values of 9.0, 8.5 and 13.2, respectively. Moreover, the dichloromethane and ethyl acetate fractions showed the highest antioxidant potential. A bioassay-guided fractionation led to the isolation and characterization of seven compounds for the first time, namely, quercetin (1), 3-methoxy quercetin (2), kaempferol (3), 3-methoxy kaempferol (4), myricetin 3,7,3'-trimethyl ether (5), lupeol (6), and stigmasterol (7). CONCLUSION: Our results indicate that P. resinosa is a source for antimicrobial and cytotoxic compounds. However, further work is required to isolate other active principles and to determine the mechanism of action.
format	Article
author	Albadawi, Dina A. A. Mothana, Ramzi Ahmed M. Khaled, Jamal Ashour, Abdelkader Elbadawy Abbas Kumar, Ashok Ahmad, Sheikh Fayaz Al-Said, Mansour Sulaiman Al-Rehaily, Adnan Jathlan M. Almusayeib, Nawal
author_facet	Albadawi, Dina A. A. Mothana, Ramzi Ahmed M. Khaled, Jamal Ashour, Abdelkader Elbadawy Abbas Kumar, Ashok Ahmad, Sheikh Fayaz Al-Said, Mansour Sulaiman Al-Rehaily, Adnan Jathlan M. Almusayeib, Nawal
author_sort	Albadawi, Dina A.
title	Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia
title_short	Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia
title_full	Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia
title_fullStr	Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia
title_full_unstemmed	Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia
title_sort	antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in saudi arabia
publisher	Taylor and Francis Ltd.
publishDate	2017
url	http://irep.iium.edu.my/63547/ http://irep.iium.edu.my/63547/ http://irep.iium.edu.my/63547/ http://irep.iium.edu.my/63547/1/Antimicrobial%2C%20anticancer%2C%20and%20antioxidant%20compounds%20from%20Premna%20resinosa%20growing%20in%20Saudi%20Arabia.pdf http://irep.iium.edu.my/63547/7/63547_Antimicrobial%2C%20anticancer%2C%20and%20antioxidant%20compounds_scopus.pdf
first_indexed	2023-09-18T21:30:07Z
last_indexed	2023-09-18T21:30:07Z
_version_	1777412455015645184

Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia

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