Antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in Saudi Arabia
CONTEXT: Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. OBJECTIVE: This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constitu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Taylor and Francis Ltd.
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/63547/ http://irep.iium.edu.my/63547/ http://irep.iium.edu.my/63547/ http://irep.iium.edu.my/63547/1/Antimicrobial%2C%20anticancer%2C%20and%20antioxidant%20compounds%20from%20Premna%20resinosa%20growing%20in%20Saudi%20Arabia.pdf http://irep.iium.edu.my/63547/7/63547_Antimicrobial%2C%20anticancer%2C%20and%20antioxidant%20compounds_scopus.pdf |
| Summary: | CONTEXT:
Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage.
OBJECTIVE:
This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constituents.
MATERIALS AND METHODS:
The methanol extract of P. resinosa aerial parts and its fractions (n-hexane, dichloromethane, ethyl acetate and n-butanol) were tested. Antimicrobial activity was tested using microdilution method against three Gram-positive and four Gram-negative bacteria. The tested concentrations ranged from 4000 to 7.8 μg/mL and MIC values were determined after 24 h incubation. Anticancer activity was evaluated against three human cancer cell lines (Daoy, HepG2 and SK-MEL28) using MTT assay. Antioxidant activity was investigated by DPPH scavenging method and β-carotene-linoleic acid assay.
RESULTS:
The greatest antimicrobial activity was exhibited by n-hexane fraction (MIC 10 μg/mL) against Staphylococcus aureus, Enterococcus faecalis, and Shigella flexneri. The n-hexane fraction induced the greatest cytotoxic activity against Daoy, HepG2, and SK-MEL28 cell lines with IC50 values of 9.0, 8.5 and 13.2, respectively. Moreover, the dichloromethane and ethyl acetate fractions showed the highest antioxidant potential. A bioassay-guided fractionation led to the isolation and characterization of seven compounds for the first time, namely, quercetin (1), 3-methoxy quercetin (2), kaempferol (3), 3-methoxy kaempferol (4), myricetin 3,7,3'-trimethyl ether (5), lupeol (6), and stigmasterol (7).
CONCLUSION:
Our results indicate that P. resinosa is a source for antimicrobial and cytotoxic compounds. However, further work is required to isolate other active principles and to determine the mechanism of action. |
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