The effects of low and high salt intake during pregnancy on the development of the offspring’s kidney in sprague-dawley rats
Background: The nephron endowment depends on both genetic and environmental factors. The intrauterine environment such as maternal high or low salt intake may directly or indirectly impair the foetal development and favour programming of diseases in later life. Objective: This study invest...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/63494/ http://irep.iium.edu.my/63494/2/MSH%202018%20Poster%20DrAzra.pdf http://irep.iium.edu.my/63494/12/63494_THE%20EFFECTS%20OF%20LOW%20AND%20HIGH%20SALT%20INTAKE.pdf |
| Summary: | Background:
The nephron endowment depends on both genetic and environmental factors. The intrauterine environment such as maternal high or low salt intake may directly or indirectly impair the foetal development and favour programming of diseases in later life.
Objective:
This study investigates the effects of different maternal salt intake on the body weight, kidney weight, and nephron number of the female offspring.
Material and methods:
Normotensive female Sprague-Dawley rats were subjected to timed pregnancy. At postnatal day 1, the body and kidney weight of the female offspring from dams on low salt diet (LSD)(0.145% NaCl, n=6), normal salt diet (NSD)(1.0% NaCl, n=6), and high salt diet (HSD)(3.0% NaCl, n=6) diet were recorded, dissected and fixed in 4% paraformaldehyde. The kidneys were then processed, cut into 6µm and stained with Haematoxylin and Eosin. The kidney sections were viewed using Leica DM750 microscope. Images were then captured using Leica ICC50HD camera and were assessed using Leica Application Suite V4 (LAS V4) software.
Results:
The body weight of the offspring from dams on HSD and LSD were significantly lower than NSD dams (5.852g ± 0.072 vs 5.508g ± 0.188 and 6.517g ± 0.076 )(P< 0.05), respectively. The kidney weight of the offspring from dams on LSD were significantly lower than NSD dams (0.0607g ± 0.0019 vs 0.0740g ± 0.0012)(P< 0.02). The glomerular number were much lower in the offspring from LSD and HSD dams than in NSD dams (8080.50 ± 706.49 vs 5256.50 ± 867.78 vs 11300.83 ± 1041.72)( P< 0.05), respectively. There was also evidence of haemorrhages in the glomerular tuft and interstitium in the offspring from HSD and LSD dams. In addition, there were comparable kidney to body weight ratio among the offspring of the LSD, HSD and NSD dams (0.0111 ± 0.0003 vs 0.0120 ± 0.0005 vs 0.0113 ± 0.0003), respectively.
Conclusion:
High and low maternal salt intake during pregnancy resulted in abnormal renal development of the offspring and birth weight is an indicative surrogate parameter for intrauterine development. |
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