Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness
The optimum conditions for immobilization of β-Glucosidase on multiwalled carbon nanotubes (MWCNTs) and Ca-alginate beads were studied. The effectiveness of the reaction of the immobilized enzyme with pnitrophenyl-β-D-glucopyranoside as substrate is evaluated. For the same substrate concentration...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyyah of Engineering International Islamic University Malaysia P.O. Box 10, 50728 Kuala Lumpur, Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/63485/ http://irep.iium.edu.my/63485/ http://irep.iium.edu.my/63485/14/63485_Optimum%20Immobilization%20of%20%CE%B2-Glucosidase%20Enzyme_baru.pdf |
| Summary: | The optimum conditions for immobilization of β-Glucosidase on multiwalled carbon nanotubes (MWCNTs)
and Ca-alginate beads were studied. The effectiveness of the reaction of the immobilized enzyme with pnitrophenyl-β-D-glucopyranoside
as substrate is evaluated. For the same substrate concentration the activity
of enzyme immobilized in Ca-alginate beads was lower than that of immobilization on MWCNTs. The
optimum immobilization conditions were found to be 17 mg MWCNT and 4 hr incubation time for MWCNTs
while for calcium-alginate gel, it was found to be 3.5 wt% Na-alginate and 2 hr incubation time. The
immobilization yield of the enzyme on MWCNTs was found to be 63.91% and that of Ca-alginate beads was
53.56%. Upon subsequent use, the residual activity of the enzyme on MWCNTs was higher than that of the
Ca-alginate beads, which lost almost 50% of the activity at the third run. Both Ca-alginate and MWCNT
immobilized enzyme were found to follow Michaelis-Menten kinetics and were well represented by Langmuir
plot which was finally used to determine Michaelis-Menten constants. The values of Vmax and Km for
MWCNTs are 4.7 μmol/mL.min and 0.833 mM respectively while for Ca-alginate the constants are 4.428
μmol/mL.min and 0.2587 mM respectively. |
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