The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken

In this study, the differences in protein expression from chicken liver between commercial broilers (CB) and indigenous chicken (IC) have been analysed by using two-dimensional gel electrophoresis (2D-PAGE). The study was conducted adjacent to consumer experience in order to find out the differenc...

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Main Authors:	Abu Hassan, Muhamad Shafiq, Saidin, Muhammad Syazwan, Hamdan, Nurul Ashima, Abd Hamid, Shafida, Muhamad Bunnori, Noraslinda
Format:	Conference or Workshop Item
Language:	English English
Published:	World Research Library 2018
Subjects:	SF Animal culture
Online Access:	http://irep.iium.edu.my/63463/ http://irep.iium.edu.my/63463/ http://irep.iium.edu.my/63463/23/63463-The%20near%20consumer%20insight%20on%20liver%20proteomics%20of%20Malaysian%20broiler.pdf http://irep.iium.edu.my/63463/16/ISD-ENSYOK-09028-6475_NORASLINDA%20ICENS.pdf

id	iium-63463
recordtype	eprints
spelling	iium-634632018-06-26T03:07:21Z http://irep.iium.edu.my/63463/ The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken Abu Hassan, Muhamad Shafiq Saidin, Muhammad Syazwan Hamdan, Nurul Ashima Abd Hamid, Shafida Muhamad Bunnori, Noraslinda SF Animal culture In this study, the differences in protein expression from chicken liver between commercial broilers (CB) and indigenous chicken (IC) have been analysed by using two-dimensional gel electrophoresis (2D-PAGE). The study was conducted adjacent to consumer experience in order to find out the differences of the protein profile in liver tissues between the two popular types of chickens that have been offered to the Malaysians consumer for over 50 years. In 2D-PAGE, six spots were successfully identified deferentially expressed in term of intensity. Those proteins are phosphoenol pyruvate carboxykinase (PEPCK) (2 spots), delta-1-pyrolline-5-carboxylate (P5CDH), soluble epoxide hydrolase (sEH), heat shock protein 90 (HSP90) and γ-actin. PEPCK and P5CDH enzymes were expressed higher in CB compared to IC, suggesting the utilization of alternative metabolic pathways for broilers’ energy metabolism. The sEH expression was found higher in CB compared to IC probably due to the process of xenobiotic metabolic activity to eliminate harmful epoxide inside the CB’s liver. HSP90 was found less in CB compared to IC, which may indicate the lack of regulation of heat stress response in CB. The higher expression of actin in IC liver suggested a good form of structure integrity in IC liver compared to CB, which may happen due to the age differences.Most of the differentially expressed proteins are involved in energy metabolism, stress regulation, and cellular structure.These differences could arise from many variables involved which may include breeds, age, feed regimen, drug treatment and rearing environment. World Research Library 2018 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/63463/23/63463-The%20near%20consumer%20insight%20on%20liver%20proteomics%20of%20Malaysian%20broiler.pdf application/pdf en http://irep.iium.edu.my/63463/16/ISD-ENSYOK-09028-6475_NORASLINDA%20ICENS.pdf Abu Hassan, Muhamad Shafiq and Saidin, Muhammad Syazwan and Hamdan, Nurul Ashima and Abd Hamid, Shafida and Muhamad Bunnori, Noraslinda (2018) The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken. In: 151st IIER International Conference, 8-9 February 2018, Osaka, Japan. http://www.worldresearchlibrary.org/up_proc/pdf/1393-152213312557-64.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	SF Animal culture
spellingShingle	SF Animal culture Abu Hassan, Muhamad Shafiq Saidin, Muhammad Syazwan Hamdan, Nurul Ashima Abd Hamid, Shafida Muhamad Bunnori, Noraslinda The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken
description	In this study, the differences in protein expression from chicken liver between commercial broilers (CB) and indigenous chicken (IC) have been analysed by using two-dimensional gel electrophoresis (2D-PAGE). The study was conducted adjacent to consumer experience in order to find out the differences of the protein profile in liver tissues between the two popular types of chickens that have been offered to the Malaysians consumer for over 50 years. In 2D-PAGE, six spots were successfully identified deferentially expressed in term of intensity. Those proteins are phosphoenol pyruvate carboxykinase (PEPCK) (2 spots), delta-1-pyrolline-5-carboxylate (P5CDH), soluble epoxide hydrolase (sEH), heat shock protein 90 (HSP90) and γ-actin. PEPCK and P5CDH enzymes were expressed higher in CB compared to IC, suggesting the utilization of alternative metabolic pathways for broilers’ energy metabolism. The sEH expression was found higher in CB compared to IC probably due to the process of xenobiotic metabolic activity to eliminate harmful epoxide inside the CB’s liver. HSP90 was found less in CB compared to IC, which may indicate the lack of regulation of heat stress response in CB. The higher expression of actin in IC liver suggested a good form of structure integrity in IC liver compared to CB, which may happen due to the age differences.Most of the differentially expressed proteins are involved in energy metabolism, stress regulation, and cellular structure.These differences could arise from many variables involved which may include breeds, age, feed regimen, drug treatment and rearing environment.
format	Conference or Workshop Item
author	Abu Hassan, Muhamad Shafiq Saidin, Muhammad Syazwan Hamdan, Nurul Ashima Abd Hamid, Shafida Muhamad Bunnori, Noraslinda
author_facet	Abu Hassan, Muhamad Shafiq Saidin, Muhammad Syazwan Hamdan, Nurul Ashima Abd Hamid, Shafida Muhamad Bunnori, Noraslinda
author_sort	Abu Hassan, Muhamad Shafiq
title	The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken
title_short	The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken
title_full	The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken
title_fullStr	The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken
title_full_unstemmed	The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken
title_sort	near consumer insight on liver proteomics of malaysian broiler in comparison with indigenous chicken
publisher	World Research Library
publishDate	2018
url	http://irep.iium.edu.my/63463/ http://irep.iium.edu.my/63463/ http://irep.iium.edu.my/63463/23/63463-The%20near%20consumer%20insight%20on%20liver%20proteomics%20of%20Malaysian%20broiler.pdf http://irep.iium.edu.my/63463/16/ISD-ENSYOK-09028-6475_NORASLINDA%20ICENS.pdf
first_indexed	2023-09-18T21:30:00Z
last_indexed	2023-09-18T21:30:00Z
_version_	1777412447409274880

The near consumer insight on liver proteomics of Malaysian broiler in comparison with indigenous chicken

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