Fabrication of ceramic membrane chromatography for biologics purification

Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study,...

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Main Authors:	Mel, Maizirwan, Abdeen, Fouad, Sopyan, Iis
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	TP155 Chemical engineering TP200 Manufacture and use of chemicals TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/6301/ http://irep.iium.edu.my/6301/1/Paper_ID891_AMPT_2009.pdf

id	iium-6301
recordtype	eprints
spelling	iium-63012011-11-21T19:52:37Z http://irep.iium.edu.my/6301/ Fabrication of ceramic membrane chromatography for biologics purification Mel, Maizirwan Abdeen, Fouad Sopyan, Iis TP155 Chemical engineering TP200 Manufacture and use of chemicals TP248.13 Biotechnology Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study, a coin-like, 13 mm diameter and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic medium. The membrane is used to replace the conventional resin-based chromatography columns. Hydroxyapatite (HA) powder was used as a material for the membrane fabrication. In this project, a HA powder was produced using starch as pore creating agents. Characterization processes were done for the ceramic membrane using the suitable apparatuses. Three parameters of the fabrication process (starch wt %, compaction pressure and sintering temperature) were manipulated to optimize the performance of the membrane. The fabricated membrane was placed in a (FPLC) system to be tested for its performance as an adsorptive membrane. (IMAC) process was run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the (NDV) was used to test the membrane’s ability to bind Histidine-tagged proteins. The optimum set of process parameters that yielded in the highest porosity and good chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and 1100°C sintering temperature. 2009-10-29 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/6301/1/Paper_ID891_AMPT_2009.pdf Mel, Maizirwan and Abdeen, Fouad and Sopyan, Iis (2009) Fabrication of ceramic membrane chromatography for biologics purification. In: Advanced materials and Processing Technologies, 26-29 October 2009, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP200 Manufacture and use of chemicals TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP200 Manufacture and use of chemicals TP248.13 Biotechnology Mel, Maizirwan Abdeen, Fouad Sopyan, Iis Fabrication of ceramic membrane chromatography for biologics purification
description	Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study, a coin-like, 13 mm diameter and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic medium. The membrane is used to replace the conventional resin-based chromatography columns. Hydroxyapatite (HA) powder was used as a material for the membrane fabrication. In this project, a HA powder was produced using starch as pore creating agents. Characterization processes were done for the ceramic membrane using the suitable apparatuses. Three parameters of the fabrication process (starch wt %, compaction pressure and sintering temperature) were manipulated to optimize the performance of the membrane. The fabricated membrane was placed in a (FPLC) system to be tested for its performance as an adsorptive membrane. (IMAC) process was run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the (NDV) was used to test the membrane’s ability to bind Histidine-tagged proteins. The optimum set of process parameters that yielded in the highest porosity and good chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and 1100°C sintering temperature.
format	Conference or Workshop Item
author	Mel, Maizirwan Abdeen, Fouad Sopyan, Iis
author_facet	Mel, Maizirwan Abdeen, Fouad Sopyan, Iis
author_sort	Mel, Maizirwan
title	Fabrication of ceramic membrane chromatography for biologics purification
title_short	Fabrication of ceramic membrane chromatography for biologics purification
title_full	Fabrication of ceramic membrane chromatography for biologics purification
title_fullStr	Fabrication of ceramic membrane chromatography for biologics purification
title_full_unstemmed	Fabrication of ceramic membrane chromatography for biologics purification
title_sort	fabrication of ceramic membrane chromatography for biologics purification
publishDate	2009
url	http://irep.iium.edu.my/6301/ http://irep.iium.edu.my/6301/1/Paper_ID891_AMPT_2009.pdf
first_indexed	2023-09-18T20:15:12Z
last_indexed	2023-09-18T20:15:12Z
_version_	1777407740828712960

Fabrication of ceramic membrane chromatography for biologics purification

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