Optimization of serum preparation in Vero cell culture using a statistical approach

Optimization of serum preparation in Vero cell culture using a statistical approach

Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA softwa...

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Main Authors: Ahmad Nor, Yusilawati, Nuhu Jaafar, Jaafar, Mel, Maizirwan
Format: Conference or Workshop Item
Language:English
Published: Universiti Malaysia Sabah 2009
Subjects:
TP155 Chemical engineering
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/6300/
http://irep.iium.edu.my/6300/1/Opt_serum_Preparation.pdf
id iium-6300
recordtype eprints
spelling iium-63002019-01-14T01:43:34Z http://irep.iium.edu.my/6300/ Optimization of serum preparation in Vero cell culture using a statistical approach Ahmad Nor, Yusilawati Nuhu Jaafar, Jaafar Mel, Maizirwan TP155 Chemical engineering TP248.13 Biotechnology Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA software. The best result obtained from the software was used to compare with different commercial serum (GIBCOTM and PAA). Local serum was prepared first by aseptically collecting 500ml whole blood from disease free cattle using 50ml anticoagulant free centrifuge tubes. Whole blood was centrifuged to separate serum and blood cells. Serum collected was treated with ultraviolet radiation and heat inactivated for 30min at 56oC. Vero cells were cultured in 25cm2 T flasks with an initial cell seeding of 4.2 x 105 cells/ml. Results obtained showed that locally made serum is comparable in terms of maximum viable cell numbers (8.0 x 105 cells/ml) compared to commercial bovine serum (8.5x 105 cell/ml and 6.78x105 cell/ml) for GIBCO and PAA respectively after four days. From the study, locally prepared serum can be substituted to the commercial sera for Vero cell culture. Universiti Malaysia Sabah 2009-08-14 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/6300/1/Opt_serum_Preparation.pdf Ahmad Nor, Yusilawati and Nuhu Jaafar, Jaafar and Mel, Maizirwan (2009) Optimization of serum preparation in Vero cell culture using a statistical approach. In: 3rd International Conference on Chemical & Bioprocess Engineering , 12th–14th August 2009, Kota Kinabalu, Sabah. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP155 Chemical engineering
TP248.13 Biotechnology
spellingShingle TP155 Chemical engineering
TP248.13 Biotechnology
Ahmad Nor, Yusilawati
Nuhu Jaafar, Jaafar
Mel, Maizirwan
Optimization of serum preparation in Vero cell culture using a statistical approach
description Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA software. The best result obtained from the software was used to compare with different commercial serum (GIBCOTM and PAA). Local serum was prepared first by aseptically collecting 500ml whole blood from disease free cattle using 50ml anticoagulant free centrifuge tubes. Whole blood was centrifuged to separate serum and blood cells. Serum collected was treated with ultraviolet radiation and heat inactivated for 30min at 56oC. Vero cells were cultured in 25cm2 T flasks with an initial cell seeding of 4.2 x 105 cells/ml. Results obtained showed that locally made serum is comparable in terms of maximum viable cell numbers (8.0 x 105 cells/ml) compared to commercial bovine serum (8.5x 105 cell/ml and 6.78x105 cell/ml) for GIBCO and PAA respectively after four days. From the study, locally prepared serum can be substituted to the commercial sera for Vero cell culture.
format Conference or Workshop Item
author Ahmad Nor, Yusilawati
Nuhu Jaafar, Jaafar
Mel, Maizirwan
author_facet Ahmad Nor, Yusilawati
Nuhu Jaafar, Jaafar
Mel, Maizirwan
author_sort Ahmad Nor, Yusilawati
title Optimization of serum preparation in Vero cell culture using a statistical approach
title_short Optimization of serum preparation in Vero cell culture using a statistical approach
title_full Optimization of serum preparation in Vero cell culture using a statistical approach
title_fullStr Optimization of serum preparation in Vero cell culture using a statistical approach
title_full_unstemmed Optimization of serum preparation in Vero cell culture using a statistical approach
title_sort optimization of serum preparation in vero cell culture using a statistical approach
publisher Universiti Malaysia Sabah
publishDate 2009
url http://irep.iium.edu.my/6300/
http://irep.iium.edu.my/6300/1/Opt_serum_Preparation.pdf
first_indexed 2023-09-18T20:15:12Z
last_indexed 2023-09-18T20:15:12Z
_version_ 1777407740693446656

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