Investigating the potential of Nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide
Background: Exposure to cyclophosphamide (CPA) for cancer treatment results in over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell inducing sperm defects. Our goal is to assess the potential effects of Nigella sativa extract (NSE) and thymoqu...
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International Islamic University Malaysia
2017
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iium-629412018-03-22T02:13:09Z http://irep.iium.edu.my/62941/ Investigating the potential of Nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Abdullah, Afif Raihan Mohamad Zahri, Syazana Kamarzaman, Saheera Q Science (General) QD Chemistry RM Therapeutics. Pharmacology Background: Exposure to cyclophosphamide (CPA) for cancer treatment results in over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell inducing sperm defects. Our goal is to assess the potential effects of Nigella sativa extract (NSE) and thymoquinone (TQ) on sperm and embryo quality following fertlization of sperm produced from germ cells which have been exposed to the damaging alkylating effects of CPA. Methods: Thirty male ICR mice were divided into six groups; (I) Vehicle-treated control (normal saline), (II) CPA-only, (III) TQ-only, (IV) NSE-only, (V) CPA followed by TQ and (VI) CPA followed by NSE. Treatment with 200mg/kg CPA and 10mg/kg of both NSE and TQ were given by intraperitoneal injection. Animals were sacrificed at 33 days by cervical dislocation and sperm from caudal epidydymis were taken for analysis and in vitro fertilization (IVF) with eggs from untreated female. Fertilization rates and embryo development were monitored for 5 days. The result were analysed by using SPSS 16.Results: TQ and NSE supplementation to CPA-exposed male mice have no significant effect (p>0.05) on the total number of sperm if compared to CPA-only exposed mice. NSE and TQ supplementation have been shown to have significant effect (p<0.05) on the percentage of motile sperm as well as the number of abnormal sperm. Four types of abnormalities of the sperm were found which includes folded sperm, amorphous, banana-like and the head lacking of the usual hook. Finally, the embryo quality shows a significant improvement by the supplementation of TQ and NSE to CPA-exposed male mice (p<0.05). Conclusion: Overall, both NSE and TQ have indicated chemopreventive potential against the cytotoxicity of cyclophosphamide on the reproductive capacity and fertility. International Islamic University Malaysia 2017-06-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62941/1/62941_Investigating%20the%20Potential%20of%20Nigella%20Sativa_article.pdf application/pdf en http://irep.iium.edu.my/62941/2/62941_Investigating%20the%20Potential%20of%20Nigella%20Sativa_scopus.pdf Abdul Rahman, Suzanah and Abdul Samat, Nadia Hanis and Ahmad, Nur Amalina and Abdullah, Afif Raihan and Mohamad Zahri, Syazana and Kamarzaman, Saheera (2017) Investigating the potential of Nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide. International Medical Journal Malaysia, 16 (1). pp. 99-106. ISSN 1823-4631 https://pdfs.semanticscholar.org/8121/8af5ee07e3d75b3b9a0510bb81e967ab1bad.pdf |
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Digital Repository |
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| institution |
International Islamic University Malaysia |
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IIUM Repository |
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Online Access |
| language |
English English |
| topic |
Q Science (General) QD Chemistry RM Therapeutics. Pharmacology |
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Q Science (General) QD Chemistry RM Therapeutics. Pharmacology Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Abdullah, Afif Raihan Mohamad Zahri, Syazana Kamarzaman, Saheera Investigating the potential of Nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide |
| description |
Background: Exposure to cyclophosphamide (CPA) for cancer treatment results in over-production of
reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell inducing sperm
defects. Our goal is to assess the potential effects of Nigella sativa extract (NSE) and thymoquinone (TQ) on
sperm and embryo quality following fertlization of sperm produced from germ cells which have been
exposed to the damaging alkylating effects of CPA. Methods: Thirty male ICR mice were divided into six
groups; (I) Vehicle-treated control (normal saline), (II) CPA-only, (III) TQ-only, (IV) NSE-only, (V) CPA
followed by TQ and (VI) CPA followed by NSE. Treatment with 200mg/kg CPA and 10mg/kg of both NSE and
TQ were given by intraperitoneal injection. Animals were sacrificed at 33 days by cervical dislocation and
sperm from caudal epidydymis were taken for analysis and in vitro fertilization (IVF) with eggs from
untreated female. Fertilization rates and embryo development were monitored for 5 days. The result were
analysed by using SPSS 16.Results: TQ and NSE supplementation to CPA-exposed male mice have no
significant effect (p>0.05) on the total number of sperm if compared to CPA-only exposed mice. NSE and TQ
supplementation have been shown to have significant effect (p<0.05) on the percentage of motile sperm as
well as the number of abnormal sperm. Four types of abnormalities of the sperm were found which includes
folded sperm, amorphous, banana-like and the head lacking of the usual hook. Finally, the embryo quality
shows a significant improvement by the supplementation of TQ and NSE to CPA-exposed male mice (p<0.05).
Conclusion: Overall, both NSE and TQ have indicated chemopreventive potential against the cytotoxicity of
cyclophosphamide on the reproductive capacity and fertility. |
| format |
Article |
| author |
Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Abdullah, Afif Raihan Mohamad Zahri, Syazana Kamarzaman, Saheera |
| author_facet |
Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Abdullah, Afif Raihan Mohamad Zahri, Syazana Kamarzaman, Saheera |
| author_sort |
Abdul Rahman, Suzanah |
| title |
Investigating the potential of Nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide |
| title_short |
Investigating the potential of Nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide |
| title_full |
Investigating the potential of Nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide |
| title_fullStr |
Investigating the potential of Nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide |
| title_full_unstemmed |
Investigating the potential of Nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide |
| title_sort |
investigating the potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide |
| publisher |
International Islamic University Malaysia |
| publishDate |
2017 |
| url |
http://irep.iium.edu.my/62941/ http://irep.iium.edu.my/62941/ http://irep.iium.edu.my/62941/1/62941_Investigating%20the%20Potential%20of%20Nigella%20Sativa_article.pdf http://irep.iium.edu.my/62941/2/62941_Investigating%20the%20Potential%20of%20Nigella%20Sativa_scopus.pdf |
| first_indexed |
2023-09-18T21:29:12Z |
| last_indexed |
2023-09-18T21:29:12Z |
| _version_ |
1777412396767248384 |