Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors
Monitoring the effectiveness of drugs on cancer cells is crucial for chemotherapeutics studies. Invitro cell-based biosensors can be used as an alternative for characteristic studies of cells’ response to drugs. Cell-based sensors provide real-time measurements and require smaller sample volumes...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Institute of Advanced Engineering and Science
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/62788/ http://irep.iium.edu.my/62788/ http://irep.iium.edu.my/62788/ http://irep.iium.edu.my/62788/1/62788_Content%20Cytotoxicity%20Studies%20of%20Colorectal%20_article.pdf http://irep.iium.edu.my/62788/2/62788_Content%20Cytotoxicity%20Studies%20of%20Colorectal%20_scopus.pdf |
| Summary: | Monitoring the effectiveness of drugs on cancer cells is crucial for chemotherapeutics studies. Invitro
cell-based biosensors can be used as an alternative for characteristic studies of cells’ response to
drugs. Cell-based sensors provide real-time measurements and require smaller sample volumes
compared to conventional T-flask measurement methods. This paper presents a biosensor that detects in
real-time, impedance variations of human colon cancer, HCT-116 cells when treated with anti-cancer
agent, 5-Fluorouracil (5-FU). Two different extracellular matrix (ECM); polyaniline and gelatin were tested
and evaluated in terms of attachment quality. Polyaniline was found to provide the best attachment for
HCT-116 cells and was used for cytotoxicity studies. Cytokinetic behavior indicated that 5-FU inhibited
HCT-116 cells at IC50 of 6.8 µg/mL. Trypan blue exclusion method for testing cell viability was used to
validate the impedance measurements, where the cancer cell concentrations were reduced to ~35% when
treated with 2.5 µg/mL, and 50% when treated with 6.8 µg/mL. The results generated by the
microfabricated impedance biosensor are comparable to the Trypan blue method since both gave similar
cell growth trend. It can be concluded that the impedance biosensor has potential to be used as an
alternative method in drug testing applications |
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