Early effects of high cholesterol diet on the kidney of an animal model

BACKGROUND: Previous studies have proven that there exists a complex association between progressive kidney damage and hypercholesterolemia. Most of them focused on the impact of chronically high blood cholesterol levels on the kidney. Information on the early effects of hypercholesterolemia on t...

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Main Authors:	Hamad Mohamed, Zenab B., Hamad Alfarisi, Hamad Abdulsalam, Abdullah, Nor Zamzila, Harun, Norra, Muhammad, Naznin, Abdul Rahim, Roslina
Format:	Article
Language:	English
Published:	PONTE Editore 2018
Subjects:	QP Physiology R Medicine (General) RB Pathology RC Internal medicine RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/62758/ http://irep.iium.edu.my/62758/ http://irep.iium.edu.my/62758/ http://irep.iium.edu.my/62758/1/article%20ponte%201515397798.pdf

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spelling	iium-627582018-07-10T20:04:40Z http://irep.iium.edu.my/62758/ Early effects of high cholesterol diet on the kidney of an animal model Hamad Mohamed, Zenab B. Hamad Alfarisi, Hamad Abdulsalam Abdullah, Nor Zamzila Harun, Norra Muhammad, Naznin Abdul Rahim, Roslina QP Physiology R Medicine (General) RB Pathology RC Internal medicine RM Therapeutics. Pharmacology BACKGROUND: Previous studies have proven that there exists a complex association between progressive kidney damage and hypercholesterolemia. Most of them focused on the impact of chronically high blood cholesterol levels on the kidney. Information on the early effects of hypercholesterolemia on the kidney is still lacking. The aim of this study is therefore to determine early effects of high cholesterol diet on the kidney in an animal model. METHODOLOGY: Ten female Sprague-Dawley rats were divided into two groups: the control group, fed with commercial rat pellet and the high cholesterol diet (HCD) group, fed with 12% cholesterol diet with 0.3% cholic acid. Biochemical analysis of the lipid profile and renal function were performed at completed 48 hours, 7 days, and 6 weeks of the experiment. The animals were sacrificed at 6 weeks and the kidneys were harvested for histological examination. RESULTS: The HCD group rats had significantly higher levels of serum total cholesterol (at 7 days and 6 weeks). The HDL-c and triglyceride levels were, however lower at 6 weeks. The mean serum creatinine level of the HCD group was increased after 48 hours and 7 days compared to control group. Histological examination of the kidney tissue of the HCD group at 6 weeks revealed segmental mesangial hypercellularity and mesangial matrix expansion of the glomeruli. CONCLUSION: The 12% cholesterol diet induced dyslipidaemia in the animal model. It resulted in acute kidney injury based on the serum creatinine at 48 hours and also 7 days. Kidney tissues examined at 6 weeks revealed changes confined to mesangial cells of the renal glomeruli. PONTE Editore 2018-03-17 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62758/1/article%20ponte%201515397798.pdf Hamad Mohamed, Zenab B. and Hamad Alfarisi, Hamad Abdulsalam and Abdullah, Nor Zamzila and Harun, Norra and Muhammad, Naznin and Abdul Rahim, Roslina (2018) Early effects of high cholesterol diet on the kidney of an animal model. PONTE, 74 (3/1). pp. 168-178. ISSN 0032-423X http://www.pontejournal.net/mainpanel/abstract.php?TOKEN=gRkgF5411G&PID=PJ-0N6NK doi: 10.21506/j.ponte.2018.3.12
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology R Medicine (General) RB Pathology RC Internal medicine RM Therapeutics. Pharmacology
spellingShingle	QP Physiology R Medicine (General) RB Pathology RC Internal medicine RM Therapeutics. Pharmacology Hamad Mohamed, Zenab B. Hamad Alfarisi, Hamad Abdulsalam Abdullah, Nor Zamzila Harun, Norra Muhammad, Naznin Abdul Rahim, Roslina Early effects of high cholesterol diet on the kidney of an animal model
description	BACKGROUND: Previous studies have proven that there exists a complex association between progressive kidney damage and hypercholesterolemia. Most of them focused on the impact of chronically high blood cholesterol levels on the kidney. Information on the early effects of hypercholesterolemia on the kidney is still lacking. The aim of this study is therefore to determine early effects of high cholesterol diet on the kidney in an animal model. METHODOLOGY: Ten female Sprague-Dawley rats were divided into two groups: the control group, fed with commercial rat pellet and the high cholesterol diet (HCD) group, fed with 12% cholesterol diet with 0.3% cholic acid. Biochemical analysis of the lipid profile and renal function were performed at completed 48 hours, 7 days, and 6 weeks of the experiment. The animals were sacrificed at 6 weeks and the kidneys were harvested for histological examination. RESULTS: The HCD group rats had significantly higher levels of serum total cholesterol (at 7 days and 6 weeks). The HDL-c and triglyceride levels were, however lower at 6 weeks. The mean serum creatinine level of the HCD group was increased after 48 hours and 7 days compared to control group. Histological examination of the kidney tissue of the HCD group at 6 weeks revealed segmental mesangial hypercellularity and mesangial matrix expansion of the glomeruli. CONCLUSION: The 12% cholesterol diet induced dyslipidaemia in the animal model. It resulted in acute kidney injury based on the serum creatinine at 48 hours and also 7 days. Kidney tissues examined at 6 weeks revealed changes confined to mesangial cells of the renal glomeruli.
format	Article
author	Hamad Mohamed, Zenab B. Hamad Alfarisi, Hamad Abdulsalam Abdullah, Nor Zamzila Harun, Norra Muhammad, Naznin Abdul Rahim, Roslina
author_facet	Hamad Mohamed, Zenab B. Hamad Alfarisi, Hamad Abdulsalam Abdullah, Nor Zamzila Harun, Norra Muhammad, Naznin Abdul Rahim, Roslina
author_sort	Hamad Mohamed, Zenab B.
title	Early effects of high cholesterol diet on the kidney of an animal model
title_short	Early effects of high cholesterol diet on the kidney of an animal model
title_full	Early effects of high cholesterol diet on the kidney of an animal model
title_fullStr	Early effects of high cholesterol diet on the kidney of an animal model
title_full_unstemmed	Early effects of high cholesterol diet on the kidney of an animal model
title_sort	early effects of high cholesterol diet on the kidney of an animal model
publisher	PONTE Editore
publishDate	2018
url	http://irep.iium.edu.my/62758/ http://irep.iium.edu.my/62758/ http://irep.iium.edu.my/62758/ http://irep.iium.edu.my/62758/1/article%20ponte%201515397798.pdf
first_indexed	2023-09-18T21:28:54Z
last_indexed	2023-09-18T21:28:54Z
_version_	1777412378307067904

Early effects of high cholesterol diet on the kidney of an animal model

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