Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity
Objective: To determine ex vivo antimalarial activity and cytotoxicity of endophytic Streptomyces SUK 08 as well as the main core structure fractionated from its crude extract. Methods: The activities of SUK 08 crude extract were evaluated by using the Plasmodium lactate dehydrogenase assay and...
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Elsevier BV
2017
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| Online Access: | http://irep.iium.edu.my/62746/ http://irep.iium.edu.my/62746/ http://irep.iium.edu.my/62746/ http://irep.iium.edu.my/62746/1/62746_Bioactive%20compounds%20fractionated%20from%20endophyte_article.pdf http://irep.iium.edu.my/62746/2/62746_Bioactive%20compounds%20fractionated%20from%20endophyte_scopus.pdf |
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iium-627462018-03-17T05:21:47Z http://irep.iium.edu.my/62746/ Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity Mohamad Zin, Noraziah Remali, Juwairiah Nasrom, Mohd Nazir Ishak, Shafariatul Akma Baba, Mohd Shukri Jalil, Juriyati QR Microbiology RA Public aspects of medicine RM Therapeutics. Pharmacology Objective: To determine ex vivo antimalarial activity and cytotoxicity of endophytic Streptomyces SUK 08 as well as the main core structure fractionated from its crude extract. Methods: The activities of SUK 08 crude extract were evaluated by using the Plasmodium lactate dehydrogenase assay and synchronization test against rodent malaria parasite Plasmodium berghei, instead of human malarial parasite Plasmodium falciparum. The cytotoxicity of the crude extract was determined by MTT assay. The crude extract was analyzed by thin-layer chromatography and gas chromatography–mass spectrophotometry. Results: The ethyl acetate crude extract showed very promising antimalarial activity with IC50 of 1.25 mg/mL. The synchronization tests showed that ethyl acetate extraction could inhibit all stages of the Plasmodium life cycle, but it was most effective at the Plasmodium ring stage. On the basis of a MTT assay on Chang Liver cells, ethyl acetate and ethanol demonstrated IC50 values of >1.0 mg/mL. The IC50 of parasitemia at 5% and 30% for this extract was lower than chloroquine. Thin-layer chromatography, with 1: 9 ratio of ethyl acetate: hexane, was used to isolate several distinct compounds. Based on gas chromatography–mass spectrophotometry analysis, three core structures were identified as cyclohexane, butyl propyl ester, and 2,3-heptanedione. Structurally, these compounds were similar to currently available antimalarial drugs. Conclusions: The results suggest that compounds isolated from Streptomyces SUK 08 are viable antimalarial drug candidates that require further investigations Elsevier BV 2017-12 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/62746/1/62746_Bioactive%20compounds%20fractionated%20from%20endophyte_article.pdf application/pdf en http://irep.iium.edu.my/62746/2/62746_Bioactive%20compounds%20fractionated%20from%20endophyte_scopus.pdf Mohamad Zin, Noraziah and Remali, Juwairiah and Nasrom, Mohd Nazir and Ishak, Shafariatul Akma and Baba, Mohd Shukri and Jalil, Juriyati (2017) Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity. Asian Pacific Journal of Tropical Biomedicine, 7 (12). pp. 1062-1066. ISSN 2221-1691 https://ac.els-cdn.com/S2221169116301678/1-s2.0-S2221169116301678-main.pdf?_tid=4a9b33eb-cd7c-4cbd-9411-c0a1f2f0dcc2&acdnat=1521262425_5ec152163c99158abdd7004e5695823e 10.1016/j.apjtb.2017.10.006 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
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IIUM Repository |
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Online Access |
| language |
English English |
| topic |
QR Microbiology RA Public aspects of medicine RM Therapeutics. Pharmacology |
| spellingShingle |
QR Microbiology RA Public aspects of medicine RM Therapeutics. Pharmacology Mohamad Zin, Noraziah Remali, Juwairiah Nasrom, Mohd Nazir Ishak, Shafariatul Akma Baba, Mohd Shukri Jalil, Juriyati Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity |
| description |
Objective: To determine ex vivo antimalarial activity and cytotoxicity of endophytic
Streptomyces SUK 08 as well as the main core structure fractionated from its crude
extract.
Methods: The activities of SUK 08 crude extract were evaluated by using the Plasmodium
lactate dehydrogenase assay and synchronization test against rodent malaria
parasite Plasmodium berghei, instead of human malarial parasite Plasmodium falciparum.
The cytotoxicity of the crude extract was determined by MTT assay. The crude
extract was analyzed by thin-layer chromatography and gas chromatography–mass
spectrophotometry.
Results: The ethyl acetate crude extract showed very promising antimalarial activity
with IC50 of 1.25 mg/mL. The synchronization tests showed that ethyl acetate extraction
could inhibit all stages of the Plasmodium life cycle, but it was most effective at the
Plasmodium ring stage. On the basis of a MTT assay on Chang Liver cells, ethyl acetate
and ethanol demonstrated IC50 values of >1.0 mg/mL. The IC50 of parasitemia at 5% and
30% for this extract was lower than chloroquine. Thin-layer chromatography, with 1: 9
ratio of ethyl acetate: hexane, was used to isolate several distinct compounds. Based on
gas chromatography–mass spectrophotometry analysis, three core structures were identified
as cyclohexane, butyl propyl ester, and 2,3-heptanedione. Structurally, these
compounds were similar to currently available antimalarial drugs.
Conclusions: The results suggest that compounds isolated from Streptomyces SUK 08
are viable antimalarial drug candidates that require further investigations |
| format |
Article |
| author |
Mohamad Zin, Noraziah Remali, Juwairiah Nasrom, Mohd Nazir Ishak, Shafariatul Akma Baba, Mohd Shukri Jalil, Juriyati |
| author_facet |
Mohamad Zin, Noraziah Remali, Juwairiah Nasrom, Mohd Nazir Ishak, Shafariatul Akma Baba, Mohd Shukri Jalil, Juriyati |
| author_sort |
Mohamad Zin, Noraziah |
| title |
Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity |
| title_short |
Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity |
| title_full |
Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity |
| title_fullStr |
Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity |
| title_full_unstemmed |
Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity |
| title_sort |
bioactive compounds fractionated from endophyte streptomyces suk 08 with promising ex-vivo antimalarial activity |
| publisher |
Elsevier BV |
| publishDate |
2017 |
| url |
http://irep.iium.edu.my/62746/ http://irep.iium.edu.my/62746/ http://irep.iium.edu.my/62746/ http://irep.iium.edu.my/62746/1/62746_Bioactive%20compounds%20fractionated%20from%20endophyte_article.pdf http://irep.iium.edu.my/62746/2/62746_Bioactive%20compounds%20fractionated%20from%20endophyte_scopus.pdf |
| first_indexed |
2023-09-18T21:28:53Z |
| last_indexed |
2023-09-18T21:28:53Z |
| _version_ |
1777412377255346176 |