Bioactive compounds fractionated from endophyte Streptomyces SUK 08 with promising ex-vivo antimalarial activity
Objective: To determine ex vivo antimalarial activity and cytotoxicity of endophytic Streptomyces SUK 08 as well as the main core structure fractionated from its crude extract. Methods: The activities of SUK 08 crude extract were evaluated by using the Plasmodium lactate dehydrogenase assay and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier BV
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/62746/ http://irep.iium.edu.my/62746/ http://irep.iium.edu.my/62746/ http://irep.iium.edu.my/62746/1/62746_Bioactive%20compounds%20fractionated%20from%20endophyte_article.pdf http://irep.iium.edu.my/62746/2/62746_Bioactive%20compounds%20fractionated%20from%20endophyte_scopus.pdf |
| Summary: | Objective: To determine ex vivo antimalarial activity and cytotoxicity of endophytic
Streptomyces SUK 08 as well as the main core structure fractionated from its crude
extract.
Methods: The activities of SUK 08 crude extract were evaluated by using the Plasmodium
lactate dehydrogenase assay and synchronization test against rodent malaria
parasite Plasmodium berghei, instead of human malarial parasite Plasmodium falciparum.
The cytotoxicity of the crude extract was determined by MTT assay. The crude
extract was analyzed by thin-layer chromatography and gas chromatography–mass
spectrophotometry.
Results: The ethyl acetate crude extract showed very promising antimalarial activity
with IC50 of 1.25 mg/mL. The synchronization tests showed that ethyl acetate extraction
could inhibit all stages of the Plasmodium life cycle, but it was most effective at the
Plasmodium ring stage. On the basis of a MTT assay on Chang Liver cells, ethyl acetate
and ethanol demonstrated IC50 values of >1.0 mg/mL. The IC50 of parasitemia at 5% and
30% for this extract was lower than chloroquine. Thin-layer chromatography, with 1: 9
ratio of ethyl acetate: hexane, was used to isolate several distinct compounds. Based on
gas chromatography–mass spectrophotometry analysis, three core structures were identified
as cyclohexane, butyl propyl ester, and 2,3-heptanedione. Structurally, these
compounds were similar to currently available antimalarial drugs.
Conclusions: The results suggest that compounds isolated from Streptomyces SUK 08
are viable antimalarial drug candidates that require further investigations |
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