Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines
Vernonia glaberrima is a medicinal plant that is used in African traditional medicine for the treatment of skin cancer. The aim of this study was to investigate the anticancer activity of V. glaberrima leaves and isolate its bioactive constituents. Crude methanolic leaves extract of V. glaberrima sh...
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| Format: | Article |
| Language: | English English English |
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Elsevier
2018
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| Online Access: | http://irep.iium.edu.my/62510/ http://irep.iium.edu.my/62510/ http://irep.iium.edu.my/62510/ http://irep.iium.edu.my/62510/1/62510_Phytoconstituents%20from%20Vernonia%20glaberrima.pdf http://irep.iium.edu.my/62510/2/62510_Phytoconstituents%20from%20Vernonia%20glaberrima_SCOPUS.pdf http://irep.iium.edu.my/62510/3/62510_Phytoconstituents%20from%20Vernonia%20glaberrima_WOS.pdf |
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iium-625102018-06-26T08:15:24Z http://irep.iium.edu.my/62510/ Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines Alhassan, A. M. Ahmed, Qamar Uddin Latip, Jalifah Shah, Syed Adnan Ali Khan, A'a Y.F. Sarian, Murni Nazira Abdul Wahab, Ridhwan Bakhtiar, M. Taher Abdullahi, Musa I. Khatib, Alfi QD Chemistry Vernonia glaberrima is a medicinal plant that is used in African traditional medicine for the treatment of skin cancer. The aim of this study was to investigate the anticancer activity of V. glaberrima leaves and isolate its bioactive constituents. Crude methanolic leaves extract of V. glaberrima showing significant cytotoxic activity against cancer cell lines was subjected to chromatographic separation, purification and hydrolysis to yield four compounds namely, nonacosanoic acid, lupeol, 5-methylcoumarin-4-β-glucoside and 4-hydroxy-5-methylcoumarin. Three of the isolated compounds showed significant cytotoxic activity against human malignant melanoma (A375) cell line (IC50: 59.18 ± 2.70 to 139.53 ± 10.79 μg/mL) and human caucasian colon adenocarcinoma (HT-29) cell line (IC50: 4.22 ± 0.13 to 20.0 ± 1.91 μg/mL) while only lupeol displayed significant activity against human breast adenocarcinoma (MCF7) (IC50: 34.15 ± 2.32 μg/mL) cell line. CDK2 receptor and CAIX were identified through molecular docking as potential targets for the bioactive compounds. The findings of this study have revealed the therapeutic potential of V. glaberrima against breast cancer, skin cancer and colorectal carcinoma, respectively and further support its traditional uses in the treatment of skin cancer. Keywords: Vernonia glaberrima; Anticancer activity; 5-Methylcoumarin-4-β-glucoside; Molecular docking; Carbonic anhydrase IX Elsevier 2018-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62510/1/62510_Phytoconstituents%20from%20Vernonia%20glaberrima.pdf application/pdf en http://irep.iium.edu.my/62510/2/62510_Phytoconstituents%20from%20Vernonia%20glaberrima_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/62510/3/62510_Phytoconstituents%20from%20Vernonia%20glaberrima_WOS.pdf Alhassan, A. M. and Ahmed, Qamar Uddin and Latip, Jalifah and Shah, Syed Adnan Ali and Khan, A'a Y.F. and Sarian, Murni Nazira and Abdul Wahab, Ridhwan and Bakhtiar, M. Taher and Abdullahi, Musa I. and Khatib, Alfi (2018) Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines. South African Journal of Botany, 116. pp. 16-24. ISSN 0254-6299 https://www.sciencedirect.com/science/article/pii/S0254629917313807 10.1016/j.sajb.2018.02.391 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
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IIUM Repository |
| collection |
Online Access |
| language |
English English English |
| topic |
QD Chemistry |
| spellingShingle |
QD Chemistry Alhassan, A. M. Ahmed, Qamar Uddin Latip, Jalifah Shah, Syed Adnan Ali Khan, A'a Y.F. Sarian, Murni Nazira Abdul Wahab, Ridhwan Bakhtiar, M. Taher Abdullahi, Musa I. Khatib, Alfi Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines |
| description |
Vernonia glaberrima is a medicinal plant that is used in African traditional medicine for the treatment of skin cancer. The aim of this study was to investigate the anticancer activity of V. glaberrima leaves and isolate its bioactive constituents. Crude methanolic leaves extract of V. glaberrima showing significant cytotoxic activity against cancer cell lines was subjected to chromatographic separation, purification and hydrolysis to yield four compounds namely, nonacosanoic acid, lupeol, 5-methylcoumarin-4-β-glucoside and 4-hydroxy-5-methylcoumarin. Three of the isolated compounds showed significant cytotoxic activity against human malignant melanoma (A375) cell line (IC50: 59.18 ± 2.70 to 139.53 ± 10.79 μg/mL) and human caucasian colon adenocarcinoma (HT-29) cell line (IC50: 4.22 ± 0.13 to 20.0 ± 1.91 μg/mL) while only lupeol displayed significant activity against human breast adenocarcinoma (MCF7) (IC50: 34.15 ± 2.32 μg/mL) cell line. CDK2 receptor and CAIX were identified through molecular docking as potential targets for the bioactive compounds. The findings of this study have revealed the therapeutic potential of V. glaberrima against breast cancer, skin cancer and colorectal carcinoma, respectively and further support its traditional uses in the treatment of skin cancer.
Keywords: Vernonia glaberrima; Anticancer activity; 5-Methylcoumarin-4-β-glucoside; Molecular docking; Carbonic anhydrase IX |
| format |
Article |
| author |
Alhassan, A. M. Ahmed, Qamar Uddin Latip, Jalifah Shah, Syed Adnan Ali Khan, A'a Y.F. Sarian, Murni Nazira Abdul Wahab, Ridhwan Bakhtiar, M. Taher Abdullahi, Musa I. Khatib, Alfi |
| author_facet |
Alhassan, A. M. Ahmed, Qamar Uddin Latip, Jalifah Shah, Syed Adnan Ali Khan, A'a Y.F. Sarian, Murni Nazira Abdul Wahab, Ridhwan Bakhtiar, M. Taher Abdullahi, Musa I. Khatib, Alfi |
| author_sort |
Alhassan, A. M. |
| title |
Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines |
| title_short |
Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines |
| title_full |
Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines |
| title_fullStr |
Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines |
| title_full_unstemmed |
Phytoconstituents from Vernonia glaberrima Welw. Ex O. Hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines |
| title_sort |
phytoconstituents from vernonia glaberrima welw. ex o. hoffm. leaves and their cytotoxic activities on a panel of human cancer cell lines |
| publisher |
Elsevier |
| publishDate |
2018 |
| url |
http://irep.iium.edu.my/62510/ http://irep.iium.edu.my/62510/ http://irep.iium.edu.my/62510/ http://irep.iium.edu.my/62510/1/62510_Phytoconstituents%20from%20Vernonia%20glaberrima.pdf http://irep.iium.edu.my/62510/2/62510_Phytoconstituents%20from%20Vernonia%20glaberrima_SCOPUS.pdf http://irep.iium.edu.my/62510/3/62510_Phytoconstituents%20from%20Vernonia%20glaberrima_WOS.pdf |
| first_indexed |
2023-09-18T21:28:35Z |
| last_indexed |
2023-09-18T21:28:35Z |
| _version_ |
1777412358134562816 |