Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles

Purpose: To compare the gene delivery effectiveness of plasmid DNA (pDNA) encapsulated within poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles with that adsorbed on PLGA nanoparticles. Methods: PLGA nanoparticles were prepared using solvent-evaporation method. To encapsulate pDNA within the p...

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Main Authors:	Doolaanea, Abd Almonem, Mansor, Nur 'Izzati, Mohd Nor, Nurul Hafizah, Mohd Shafri, Mohd Affendi, Mohamed, Farahidah
Format:	Article
Language:	English English English
Published:	Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria 2018
Subjects:	RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/62375/ http://irep.iium.edu.my/62375/ http://irep.iium.edu.my/62375/ http://irep.iium.edu.my/62375/1/Comparative%20assessment%20of%20plasmid%20DNA%20delivery.pdf http://irep.iium.edu.my/62375/7/62375-Comparative%20assessment%20of%20plasmid%20DNA%20delivery-SCOPUS.pdf http://irep.iium.edu.my/62375/13/62375_Comparative%20assessment%20of%20plasmid%20DNA%20delivery%20by%20encapsulation%20within%20or%20adsorbed%20on%20poly%20%28D%2C%20L-lactide-co-glycolide%29%20nanoparticles_WOS.pdf

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spelling	iium-623752019-01-24T02:14:36Z http://irep.iium.edu.my/62375/ Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohd Shafri, Mohd Affendi Mohamed, Farahidah RS192 Materia Medica-Pharmaceutical Technology Purpose: To compare the gene delivery effectiveness of plasmid DNA (pDNA) encapsulated within poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles with that adsorbed on PLGA nanoparticles. Methods: PLGA nanoparticles were prepared using solvent-evaporation method. To encapsulate pDNA within the particles, it was first complexed with cetyltrimethylammonium bromide (CTAB) and then added to the oil phase during the synthesis. For the adsorption, PLGA nanoparticles were first modified with either CTAB or chitosan and then pDNA was adsorbed on the particle surface by electrostatic interaction. Results: Nanoparticles encapsulating pDNA exhibited better plasmid loading and protection with significantly lower burst release (p < 0.05) compared to that of the nanoparticles with adsorbed plasmid. Cell uptake of chitosan-modified nanoparticles by murine neuroblastoma (N2a) cells was significantly (p < 0.05) higher than that of chitosan-free nanoparticles. Nanoparticles encapsulating pDNA showed higher transfection efficiency (p < 0.05) in N2a cells. Conclusion: Encapsulation of pDNA within PLGA nanoparticles presents a potential strategy for gene delivery that is superior to pDNA adsorbed on the nanoparticle surface. In addition, encapsulation keeps the particle surface free for further modifications such as the addition of targeting ligands. Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria 2018-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62375/1/Comparative%20assessment%20of%20plasmid%20DNA%20delivery.pdf application/pdf en http://irep.iium.edu.my/62375/7/62375-Comparative%20assessment%20of%20plasmid%20DNA%20delivery-SCOPUS.pdf application/pdf en http://irep.iium.edu.my/62375/13/62375_Comparative%20assessment%20of%20plasmid%20DNA%20delivery%20by%20encapsulation%20within%20or%20adsorbed%20on%20poly%20%28D%2C%20L-lactide-co-glycolide%29%20nanoparticles_WOS.pdf Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Mohd Nor, Nurul Hafizah and Mohd Shafri, Mohd Affendi and Mohamed, Farahidah (2018) Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles. Tropical Journal of Pharmaceutical Research, 17 (1). pp. 1-10. ISSN 1596-5996 E-ISSN 1596-9827 https://www.tjpr.org/admin/12389900798187/2018_17_1_1.pdf 10.4314/tjpr.v17i1.1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohd Shafri, Mohd Affendi Mohamed, Farahidah Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles
description	Purpose: To compare the gene delivery effectiveness of plasmid DNA (pDNA) encapsulated within poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles with that adsorbed on PLGA nanoparticles. Methods: PLGA nanoparticles were prepared using solvent-evaporation method. To encapsulate pDNA within the particles, it was first complexed with cetyltrimethylammonium bromide (CTAB) and then added to the oil phase during the synthesis. For the adsorption, PLGA nanoparticles were first modified with either CTAB or chitosan and then pDNA was adsorbed on the particle surface by electrostatic interaction. Results: Nanoparticles encapsulating pDNA exhibited better plasmid loading and protection with significantly lower burst release (p < 0.05) compared to that of the nanoparticles with adsorbed plasmid. Cell uptake of chitosan-modified nanoparticles by murine neuroblastoma (N2a) cells was significantly (p < 0.05) higher than that of chitosan-free nanoparticles. Nanoparticles encapsulating pDNA showed higher transfection efficiency (p < 0.05) in N2a cells. Conclusion: Encapsulation of pDNA within PLGA nanoparticles presents a potential strategy for gene delivery that is superior to pDNA adsorbed on the nanoparticle surface. In addition, encapsulation keeps the particle surface free for further modifications such as the addition of targeting ligands.
format	Article
author	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohd Shafri, Mohd Affendi Mohamed, Farahidah
author_facet	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohd Shafri, Mohd Affendi Mohamed, Farahidah
author_sort	Doolaanea, Abd Almonem
title	Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles
title_short	Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles
title_full	Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles
title_fullStr	Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles
title_full_unstemmed	Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles
title_sort	comparative assessment of plasmid dna delivery by encapsulation within or adsorbed on poly (d, l-lactide-co-glycolide) nanoparticles
publisher	Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
publishDate	2018
url	http://irep.iium.edu.my/62375/ http://irep.iium.edu.my/62375/ http://irep.iium.edu.my/62375/ http://irep.iium.edu.my/62375/1/Comparative%20assessment%20of%20plasmid%20DNA%20delivery.pdf http://irep.iium.edu.my/62375/7/62375-Comparative%20assessment%20of%20plasmid%20DNA%20delivery-SCOPUS.pdf http://irep.iium.edu.my/62375/13/62375_Comparative%20assessment%20of%20plasmid%20DNA%20delivery%20by%20encapsulation%20within%20or%20adsorbed%20on%20poly%20%28D%2C%20L-lactide-co-glycolide%29%20nanoparticles_WOS.pdf
first_indexed	2023-09-18T21:28:24Z
last_indexed	2023-09-18T21:28:24Z
_version_	1777412346174504960

Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles

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