Optimum media compositions for bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates
Bioprotein is one of the useful products obtained from biotechnology invention. It is a promising replacement for the commercial fish feed supplement. In this study, the enrichment of the bioprotein content after solid state fermentation using palm kernel cake and seaweed by the white rot fungus:...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Food Science & Technology, Universiti Putra Malaysia
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/62309/ http://irep.iium.edu.my/62309/ http://irep.iium.edu.my/62309/1/%2816%29.pdf |
| Summary: | Bioprotein is one of the useful products obtained from biotechnology invention. It is a
promising replacement for the commercial fish feed supplement. In this study, the enrichment
of the bioprotein content after solid state fermentation using palm kernel cake and seaweed by
the white rot fungus: Phanerochaete chrysoporium and yeast: Candida utilis was carried out.
The growth media components were selected from 11 types of media using Plackett-Burman
design (hereinafter PBD) and were optimized by one-factor-at-a-time (OFAT) method with
bioprotein concentration (mg/g) as the response. From the screening result using PBD, three
media components, namely K2
HPO4
, CuSO4
.5H2
O and MnSO4
.H2
O were selected for further
optimization using OFAT method because of their positive contributions to the response. The
final results showed that 5.0 g/L K2
HPO4
, 3.0 g/L CuSO4
.5H2
O and 0.1 g/L MnSO4
.H2
O were
there to be the optimum media constituents with 9.0 g/L, MgSO4
.7H2
O, 0.1 g/L, CaCl2
.H2
O, 3.0
g/L FeSO4
.7H2
O and 3.0 g/L peptone as fixed compositions. At this optimum concentration, the
protein increment of 11% was observed as compared to the results determined in the screening
using PBD. The study revealed the benefits of using mixed cultures in improving the protein
concentrations which can be used as nutritious fish feed. |
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