Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar

We have recently shown that Latent transforming growth factor-beta 1 binding protein-2 (LTBP-2) has a single high-affinity binding site for fibroblast growth factor-2 (FGF-2) and that LTBP-2 blocks FGF-2 induced cell proliferation. Both proteins showed strong co-localisation within keloid skin from...

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Main Authors:	Mohamed Sideek, Mohamed Arshad, Teia, Abdulrahman, Kopecki, Zlatko, Cowin, Allison J., Gibson, Mark A.
Format:	Article
Language:	English English
Published:	Springer Netherlands 2016
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/62197/ http://irep.iium.edu.my/62197/ http://irep.iium.edu.my/62197/ http://irep.iium.edu.my/62197/1/LTBP-2%20Fibrotic%20Paper%20-%20J%20Mol%20Hist.pdf http://irep.iium.edu.my/62197/7/62197_Co-localization%20of%20LTBP-2%20with%20FGF-2%20in%20fibrotic_scopus.pdf

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spelling	iium-621972018-02-28T01:34:37Z http://irep.iium.edu.my/62197/ Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar Mohamed Sideek, Mohamed Arshad Teia, Abdulrahman Kopecki, Zlatko Cowin, Allison J. Gibson, Mark A. RB Pathology We have recently shown that Latent transforming growth factor-beta 1 binding protein-2 (LTBP-2) has a single high-affinity binding site for fibroblast growth factor-2 (FGF-2) and that LTBP-2 blocks FGF-2 induced cell proliferation. Both proteins showed strong co-localisation within keloid skin from a single patient. In the current study, using confocal microscopy, we have investigated the distribution of the two proteins in normal and fibrotic skin samples including normal scar tissue, hypertrophic scars and keloids from multiple patients. Consistently, little staining for either protein was detected in normal adult skin and normal scar samples but extensive co-localisation of the two proteins was observed in multiple examples of hypertrophic scars and keloids. LTBP-2 and FGF-2 were co-localised to fine fibrous elements within the extracellular matrix identified as elastic fibres by immunostaining with anti-fibrillin-1 and anti-elastin antibodies. Furthermore, qPCR analysis of RNA samples from multiple patients confirmed dramatically increased expression of LTBP-2 and FGF-2, similar TGF-beta 1, in hypertrophic scar compared to normal skin and scar tissue. Overall the results suggest that elevated LTBP-2 may bind and sequester FGF-2 on elastic fibres in fibrotic tissues and modulate FGF-2’s influence on the repair and healing processes. Springer Netherlands 2016-02-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62197/1/LTBP-2%20Fibrotic%20Paper%20-%20J%20Mol%20Hist.pdf application/pdf en http://irep.iium.edu.my/62197/7/62197_Co-localization%20of%20LTBP-2%20with%20FGF-2%20in%20fibrotic_scopus.pdf Mohamed Sideek, Mohamed Arshad and Teia, Abdulrahman and Kopecki, Zlatko and Cowin, Allison J. and Gibson, Mark A. (2016) Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar. Journal of Molecular Histology, 47 (1). pp. 35-45. ISSN 1567-2379 http://download.springer.com/static/pdf/221/art%253A10.1007%252Fs10735-015-9645-0.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs10735-015-9645-0&token 10.1007/s10735-015-9645-0
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RB Pathology
spellingShingle	RB Pathology Mohamed Sideek, Mohamed Arshad Teia, Abdulrahman Kopecki, Zlatko Cowin, Allison J. Gibson, Mark A. Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar
description	We have recently shown that Latent transforming growth factor-beta 1 binding protein-2 (LTBP-2) has a single high-affinity binding site for fibroblast growth factor-2 (FGF-2) and that LTBP-2 blocks FGF-2 induced cell proliferation. Both proteins showed strong co-localisation within keloid skin from a single patient. In the current study, using confocal microscopy, we have investigated the distribution of the two proteins in normal and fibrotic skin samples including normal scar tissue, hypertrophic scars and keloids from multiple patients. Consistently, little staining for either protein was detected in normal adult skin and normal scar samples but extensive co-localisation of the two proteins was observed in multiple examples of hypertrophic scars and keloids. LTBP-2 and FGF-2 were co-localised to fine fibrous elements within the extracellular matrix identified as elastic fibres by immunostaining with anti-fibrillin-1 and anti-elastin antibodies. Furthermore, qPCR analysis of RNA samples from multiple patients confirmed dramatically increased expression of LTBP-2 and FGF-2, similar TGF-beta 1, in hypertrophic scar compared to normal skin and scar tissue. Overall the results suggest that elevated LTBP-2 may bind and sequester FGF-2 on elastic fibres in fibrotic tissues and modulate FGF-2’s influence on the repair and healing processes.
format	Article
author	Mohamed Sideek, Mohamed Arshad Teia, Abdulrahman Kopecki, Zlatko Cowin, Allison J. Gibson, Mark A.
author_facet	Mohamed Sideek, Mohamed Arshad Teia, Abdulrahman Kopecki, Zlatko Cowin, Allison J. Gibson, Mark A.
author_sort	Mohamed Sideek, Mohamed Arshad
title	Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar
title_short	Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar
title_full	Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar
title_fullStr	Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar
title_full_unstemmed	Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar
title_sort	co-localization of ltbp-2 with fgf-2 in fibrotic human keloid and hypertrophic scar
publisher	Springer Netherlands
publishDate	2016
url	http://irep.iium.edu.my/62197/ http://irep.iium.edu.my/62197/ http://irep.iium.edu.my/62197/ http://irep.iium.edu.my/62197/1/LTBP-2%20Fibrotic%20Paper%20-%20J%20Mol%20Hist.pdf http://irep.iium.edu.my/62197/7/62197_Co-localization%20of%20LTBP-2%20with%20FGF-2%20in%20fibrotic_scopus.pdf
first_indexed	2023-09-18T21:28:11Z
last_indexed	2023-09-18T21:28:11Z
_version_	1777412332811452416

Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar

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