Regulating the molar fraction of 4-hydroxybutyrate in Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological fermentation and enzymatic degradation
The regulation of 4-hydroxybutyrate (4HB) molar fraction in the poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] of a local isolate Cupriavidus sp. USMAA1020 was attempted by employing a feeding strategy through fed-batch fermentation in 100-L fermenter. The growth of Cupriavidus sp...
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| Language: | English English |
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Springer Verlag
2011
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| Online Access: | http://irep.iium.edu.my/62130/ http://irep.iium.edu.my/62130/ http://irep.iium.edu.my/62130/1/62130_Regulating%20the%20molar%20fraction%20of%204-hydroxybutyrate.pdf http://irep.iium.edu.my/62130/2/62130_Regulating%20the%20molar%20fraction%20of%204-hydroxybutyrate_SCOPUS.pdf |
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iium-621302018-04-23T03:58:02Z http://irep.iium.edu.my/62130/ Regulating the molar fraction of 4-hydroxybutyrate in Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological fermentation and enzymatic degradation Ansari, Nor Faezah Amirul, Al-Ashraf Abdullah Q Science (General) The regulation of 4-hydroxybutyrate (4HB) molar fraction in the poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] of a local isolate Cupriavidus sp. USMAA1020 was attempted by employing a feeding strategy through fed-batch fermentation in 100-L fermenter. The growth of Cupriavidus sp. USMAA1020 was enhanced by frequently feeding carbon and nitrogen at a ratio of 5 (C/N 5) using a DO-stat with cascade mode at 20% (v/v) dissolved oxygen (DO). The feeding of C/N 5 and the use of the DOstat mode were able to regulate the 4HB composition from 0–67 mol% by sequential feeding of c-butyrolactone and supplementing oleic acid. A high 4HB molar fraction of 67 mol% with a PHA concentration of 5.2 g/L was successfully obtained by employing this feeding strategy. Notably, enzymatic degradation carried out enhanced the 4HB composition of the copolymer synthesized. PHB depolymerase enzyme from Acidovorax sp. was used to degrade this P(3HB-co-70-mol%4HB) copolymer and the 4HB composition could be increased up to 83 mol%. The degradation process was observed by monitoring the timedependent change in the weight loss of copolymer films. The percentage of weight loss of solvent-cast film increased proportionally up to 19% within 3 h, whereas salt-leached films showed 90% of weight loss within 3 h of incubation and were completely degraded by 4 h. The molecular weight (Mn) of the films treated with enzyme demonstrated a slight decrease. SEM observation exhibited a rough surface morphology of the copolymer degraded with depolymerase enzyme. Springer Verlag 2011-02-26 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62130/1/62130_Regulating%20the%20molar%20fraction%20of%204-hydroxybutyrate.pdf application/pdf en http://irep.iium.edu.my/62130/2/62130_Regulating%20the%20molar%20fraction%20of%204-hydroxybutyrate_SCOPUS.pdf Ansari, Nor Faezah and Amirul, Al-Ashraf Abdullah (2011) Regulating the molar fraction of 4-hydroxybutyrate in Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological fermentation and enzymatic degradation. World Journal of Microbiology and Biotechnology, 27 (10). pp. 2455-2459. ISSN 0959-3993 https://link.springer.com/article/10.1007/s11274-011-0697-3 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
Q Science (General) |
| spellingShingle |
Q Science (General) Ansari, Nor Faezah Amirul, Al-Ashraf Abdullah Regulating the molar fraction of 4-hydroxybutyrate in Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological fermentation and enzymatic degradation |
| description |
The regulation of 4-hydroxybutyrate (4HB)
molar fraction in the poly(3-hydroxybutyrate-co-4-hydroxybutyrate)
[P(3HB-co-4HB)] of a local isolate Cupriavidus
sp. USMAA1020 was attempted by employing a feeding
strategy through fed-batch fermentation in 100-L fermenter.
The growth of Cupriavidus sp. USMAA1020 was enhanced
by frequently feeding carbon and nitrogen at a ratio of 5 (C/N
5) using a DO-stat with cascade mode at 20% (v/v) dissolved
oxygen (DO). The feeding of C/N 5 and the use of the DOstat
mode were able to regulate the 4HB composition from
0–67 mol% by sequential feeding of c-butyrolactone and
supplementing oleic acid. A high 4HB molar fraction of
67 mol% with a PHA concentration of 5.2 g/L was successfully
obtained by employing this feeding strategy.
Notably, enzymatic degradation carried out enhanced the
4HB composition of the copolymer synthesized. PHB
depolymerase enzyme from Acidovorax sp. was used to
degrade this P(3HB-co-70-mol%4HB) copolymer and the
4HB composition could be increased up to 83 mol%. The
degradation process was observed by monitoring the timedependent
change in the weight loss of copolymer films. The
percentage of weight loss of solvent-cast film increased
proportionally up to 19% within 3 h, whereas salt-leached
films showed 90% of weight loss within 3 h of incubation
and were completely degraded by 4 h. The molecular weight
(Mn) of the films treated with enzyme demonstrated a slight
decrease. SEM observation exhibited a rough surface morphology
of the copolymer degraded with depolymerase
enzyme. |
| format |
Article |
| author |
Ansari, Nor Faezah Amirul, Al-Ashraf Abdullah |
| author_facet |
Ansari, Nor Faezah Amirul, Al-Ashraf Abdullah |
| author_sort |
Ansari, Nor Faezah |
| title |
Regulating the molar fraction of 4-hydroxybutyrate
in Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological
fermentation and enzymatic degradation |
| title_short |
Regulating the molar fraction of 4-hydroxybutyrate
in Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological
fermentation and enzymatic degradation |
| title_full |
Regulating the molar fraction of 4-hydroxybutyrate
in Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological
fermentation and enzymatic degradation |
| title_fullStr |
Regulating the molar fraction of 4-hydroxybutyrate
in Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological
fermentation and enzymatic degradation |
| title_full_unstemmed |
Regulating the molar fraction of 4-hydroxybutyrate
in Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological
fermentation and enzymatic degradation |
| title_sort |
regulating the molar fraction of 4-hydroxybutyrate
in poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by biological
fermentation and enzymatic degradation |
| publisher |
Springer Verlag |
| publishDate |
2011 |
| url |
http://irep.iium.edu.my/62130/ http://irep.iium.edu.my/62130/ http://irep.iium.edu.my/62130/1/62130_Regulating%20the%20molar%20fraction%20of%204-hydroxybutyrate.pdf http://irep.iium.edu.my/62130/2/62130_Regulating%20the%20molar%20fraction%20of%204-hydroxybutyrate_SCOPUS.pdf |
| first_indexed |
2023-09-18T21:28:05Z |
| last_indexed |
2023-09-18T21:28:05Z |
| _version_ |
1777412326566133760 |