Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications
Polyhydroxyalkanoates (PHAs) are hydrophobic biodegradable thermoplastics that have received considerable attention in biomedical applications due to their biocompatibility, mechanical properties, and biodegradability. In this study, the degradation rate was regulated by optimizing the interactio...
| Main Authors: | , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer Verlag
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/62129/ http://irep.iium.edu.my/62129/ http://irep.iium.edu.my/62129/ http://irep.iium.edu.my/62129/1/faezah%20ansari%202013-degradation.pdf http://irep.iium.edu.my/62129/7/62129_Preparation%20and%20characterization_SCOPUS.pdf |
| Summary: | Polyhydroxyalkanoates (PHAs) are hydrophobic biodegradable thermoplastics
that have received considerable attention in biomedical applications due to their
biocompatibility, mechanical properties, and biodegradability. In this study, the degradation
rate was regulated by optimizing the interaction of parameters that influence
the enzymatic degradation of P(3HB) film using response surface methodology
(RSM). The RSM model was experimentally validated yielding a maximum 21 %
weight loss, which represents onefold increment in percentage weight loss in comparison
with the conventional method. By using the optimized condition, the enzymatic
degradation by an extracellular PHA depolymerase from Acidovorax sp. DP5 was
studied at 37 °C and pH 9.0 on different types of PHA films with various monomer
compositions. Surface modification of scaffold was employed using enzymatic technique
to create highly porous scaffold with a large surface to volume ratio, which
makes them attractive as potential tissue scaffold in biomedical field. Scanning
electron microscopy revealed that the surface of salt-leached films was more porous
compared with the solvent-cast films, and hence, increased the degradation rate of
salt-leached films. Apparently, enzymatic degradation behaviors of PHA films were
determined by several factors such as monomer composition, crystallinity, molecular
weight, porosity, and roughness of the surface. The hydrophilicity and water uptake of
degraded salt-leached film of P(3HB-co-70%4HB) were enhanced by incorporating
chitosan or alginate. Salt-leached technique followed by partial enzymatic degradation
would enhance the cell attachment and suitable for biomedical as a scaffold. |
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