The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes

The increase incidence in diabetes-and obesity-related diseases in developed and developing countries has driven serious efforts towards the discovery of adipogenic differentiation-inhibitory compounds in natural products. Mangostins and triterpenoids from Garcinia have been reported to contain a wi...

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Main Authors:	Mohamed Amiroudine, Mohamed Zaffar Ali, Bakhtiar, Muhammad Taher
Format:	Book
Language:	English
Published:	IIUM Press, International Islamic University Malaysia 2017
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/62112/ http://irep.iium.edu.my/62112/ http://irep.iium.edu.my/62112/1/62112_The%20antihyperglycaemic%20and%20antiobesity%20effects.pdf

id	iium-62112
recordtype	eprints
spelling	iium-621122018-10-03T06:46:55Z http://irep.iium.edu.my/62112/ The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, Muhammad Taher RS Pharmacy and materia medica The increase incidence in diabetes-and obesity-related diseases in developed and developing countries has driven serious efforts towards the discovery of adipogenic differentiation-inhibitory compounds in natural products. Mangostins and triterpenoids from Garcinia have been reported to contain a wide range of bioactivities. However, its antidiabetic and antiobesity activity has not been previously addressed. With regard to the fact, this research is designed to study the antihperglycaemic and antiobesity effects of selected phytochemicals isolated from Garcinia malaccensis namely beta-mangostin and cycloartane triterpenoid. The compounds were tested for their antihyperglycaemic and antiobesity effects in 3T3-L1 adipocytes. In this study, we first elucidated the effects the compounds on the lipid accumulation of 3T3-L1 preadipocytes by using Oil Red O staining. The ability of the cell to uptake glucose was measured by liquid scintillation counter. Peroxisome proliferator-activated receptors gamma (PPARy), glucose transporter four (GLUT4) and leptin genes expression was determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The results showed that β-mangostin reduced the triglyceride accumulation in 3T3-L1 adipocytes with highest lipid inhibition activity was observed at 50 μM concentration (p<0.05) when compared to MDI (0.5 mM 3-isobutyl-1-methyl-xanthine (IBMX), 0.25 mM dexamethasone, 1 μg/mL insulin) treated cells. On the other hand, treatment with cycloartane triterpenoid significantly induced lipid accumulation. Analyses of 2-deoxy-D-[3H] glucose uptake activities showed that all the compounds including the MDI, metformin and sodium orthovanadate (positive control) significantly (p<0.05) improved the glucose uptake when compared to the basal cell. In addition, evaluation by using the adipolysis kit (Cayman Chemicals) showed that β-mangostin increases the amount of free fatty acid (FFA) release. Further evaluation by the gene expression analysis showed that β-mangostin may reduce lipid accumulation by inhibition of the expression of PPARγ genes. In addition, induction of glucose uptake and free fatty acid release by β-mangostin was accompanied by increasing of mRNA expression of GLUT4 and leptin. Interestingly, mature 3T3-L1 cells treated with cycloartane triterpenoid was shown to enhance PPARγ and GLUT4 gene expression and decreased leptin expression. As a conclusion, these line of evidences indicated that β-mangostin and cycloartane triterpenoid derived from Garcinia malaccensis may become an interesting candidate for the prevention of metabolic disorders such as diabetes and obesity. IIUM Press, International Islamic University Malaysia 2017 Book PeerReviewed application/pdf en http://irep.iium.edu.my/62112/1/62112_The%20antihyperglycaemic%20and%20antiobesity%20effects.pdf Mohamed Amiroudine, Mohamed Zaffar Ali and Bakhtiar, Muhammad Taher (2017) The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes. IIUM Press, International Islamic University Malaysia, Kuala Lumpur. ISBN 978-967-418-565-7 http://iiumpress.iium.edu.my/bookshop
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, Muhammad Taher The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes
description	The increase incidence in diabetes-and obesity-related diseases in developed and developing countries has driven serious efforts towards the discovery of adipogenic differentiation-inhibitory compounds in natural products. Mangostins and triterpenoids from Garcinia have been reported to contain a wide range of bioactivities. However, its antidiabetic and antiobesity activity has not been previously addressed. With regard to the fact, this research is designed to study the antihperglycaemic and antiobesity effects of selected phytochemicals isolated from Garcinia malaccensis namely beta-mangostin and cycloartane triterpenoid. The compounds were tested for their antihyperglycaemic and antiobesity effects in 3T3-L1 adipocytes. In this study, we first elucidated the effects the compounds on the lipid accumulation of 3T3-L1 preadipocytes by using Oil Red O staining. The ability of the cell to uptake glucose was measured by liquid scintillation counter. Peroxisome proliferator-activated receptors gamma (PPARy), glucose transporter four (GLUT4) and leptin genes expression was determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The results showed that β-mangostin reduced the triglyceride accumulation in 3T3-L1 adipocytes with highest lipid inhibition activity was observed at 50 μM concentration (p<0.05) when compared to MDI (0.5 mM 3-isobutyl-1-methyl-xanthine (IBMX), 0.25 mM dexamethasone, 1 μg/mL insulin) treated cells. On the other hand, treatment with cycloartane triterpenoid significantly induced lipid accumulation. Analyses of 2-deoxy-D-[3H] glucose uptake activities showed that all the compounds including the MDI, metformin and sodium orthovanadate (positive control) significantly (p<0.05) improved the glucose uptake when compared to the basal cell. In addition, evaluation by using the adipolysis kit (Cayman Chemicals) showed that β-mangostin increases the amount of free fatty acid (FFA) release. Further evaluation by the gene expression analysis showed that β-mangostin may reduce lipid accumulation by inhibition of the expression of PPARγ genes. In addition, induction of glucose uptake and free fatty acid release by β-mangostin was accompanied by increasing of mRNA expression of GLUT4 and leptin. Interestingly, mature 3T3-L1 cells treated with cycloartane triterpenoid was shown to enhance PPARγ and GLUT4 gene expression and decreased leptin expression. As a conclusion, these line of evidences indicated that β-mangostin and cycloartane triterpenoid derived from Garcinia malaccensis may become an interesting candidate for the prevention of metabolic disorders such as diabetes and obesity.
format	Book
author	Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, Muhammad Taher
author_facet	Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, Muhammad Taher
author_sort	Mohamed Amiroudine, Mohamed Zaffar Ali
title	The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes
title_short	The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes
title_full	The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes
title_fullStr	The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes
title_full_unstemmed	The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes
title_sort	antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes
publisher	IIUM Press, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/62112/ http://irep.iium.edu.my/62112/ http://irep.iium.edu.my/62112/1/62112_The%20antihyperglycaemic%20and%20antiobesity%20effects.pdf
first_indexed	2023-09-18T21:28:03Z
last_indexed	2023-09-18T21:28:03Z
_version_	1777412324966006784

The antihyperglycaemic and antiobesity effects of selected compounds from garcinia malaccensis on 3t3-l1 adipocytes

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