Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment

Aortic scaffolds prepared using sonication decellularization treatment has provided a successful medium for repopulation with vascular smooth muscle cells (VSMCs). The objective of this study is to explore the potential of tissue decellularization using ultrasonication treatment and its recellulariz...

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Main Authors:	Ismail, Nurul Fitriatul Akmal, Sha'ban, Munirah, Noor Azmi, Azran Azhim
Format:	Conference or Workshop Item
Language:	English English
Published:	Institute of Electrical and Electronics Engineers Inc. 2017
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/1/61883_Evaluation%20of%20Recellularization%20on%20Decellularized.pdf http://irep.iium.edu.my/61883/2/61883_Evaluation%20of%20Recellularization%20on%20Decellularized_SCOPUS.pdf

id	iium-61883
recordtype	eprints
spelling	iium-618832018-06-26T04:24:51Z http://irep.iium.edu.my/61883/ Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment Ismail, Nurul Fitriatul Akmal Sha'ban, Munirah Noor Azmi, Azran Azhim TP248.13 Biotechnology Aortic scaffolds prepared using sonication decellularization treatment has provided a successful medium for repopulation with vascular smooth muscle cells (VSMCs). The objective of this study is to explore the potential of tissue decellularization using ultrasonication treatment and its recellularization before implantation of the cell-seeded scaffolds into host. Aorta tissue samples are decellularized in 2% SDS with sonication for 10 hours and compared with the native tissues. The 4',6-diamidino-2-phenylindole (DAPI) staining was used to evaluate the decellularization and Hematoxylin-Eosin (H-E) staining was used to compare the VSMCs infiltrations onto the decellularized tissues at day-0 and day-6 after cell-seeding. The results histologically showed complete DNA removal from scaffolds after decellularization and subsequent recellularization resulted in successful VSMCs infiltration. Accordingly, the decellularized tissues treated with 2% SDS in sonication demonstrated successful VSMCs repopulation afterward and is speculated to have less toxicity and able to be effectively implanted into host. Institute of Electrical and Electronics Engineers Inc. 2017-07 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/61883/1/61883_Evaluation%20of%20Recellularization%20on%20Decellularized.pdf application/pdf en http://irep.iium.edu.my/61883/2/61883_Evaluation%20of%20Recellularization%20on%20Decellularized_SCOPUS.pdf Ismail, Nurul Fitriatul Akmal and Sha'ban, Munirah and Noor Azmi, Azran Azhim (2017) Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment. In: 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2017), 11th-15th July 2017, Jeju Island, South Korea. http://ieeexplore.ieee.org/document/8037261/ 10.1109/EMBC.2017.8037261
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ismail, Nurul Fitriatul Akmal Sha'ban, Munirah Noor Azmi, Azran Azhim Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment
description	Aortic scaffolds prepared using sonication decellularization treatment has provided a successful medium for repopulation with vascular smooth muscle cells (VSMCs). The objective of this study is to explore the potential of tissue decellularization using ultrasonication treatment and its recellularization before implantation of the cell-seeded scaffolds into host. Aorta tissue samples are decellularized in 2% SDS with sonication for 10 hours and compared with the native tissues. The 4',6-diamidino-2-phenylindole (DAPI) staining was used to evaluate the decellularization and Hematoxylin-Eosin (H-E) staining was used to compare the VSMCs infiltrations onto the decellularized tissues at day-0 and day-6 after cell-seeding. The results histologically showed complete DNA removal from scaffolds after decellularization and subsequent recellularization resulted in successful VSMCs infiltration. Accordingly, the decellularized tissues treated with 2% SDS in sonication demonstrated successful VSMCs repopulation afterward and is speculated to have less toxicity and able to be effectively implanted into host.
format	Conference or Workshop Item
author	Ismail, Nurul Fitriatul Akmal Sha'ban, Munirah Noor Azmi, Azran Azhim
author_facet	Ismail, Nurul Fitriatul Akmal Sha'ban, Munirah Noor Azmi, Azran Azhim
author_sort	Ismail, Nurul Fitriatul Akmal
title	Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment
title_short	Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment
title_full	Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment
title_fullStr	Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment
title_full_unstemmed	Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment
title_sort	evaluation of recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment
publisher	Institute of Electrical and Electronics Engineers Inc.
publishDate	2017
url	http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/1/61883_Evaluation%20of%20Recellularization%20on%20Decellularized.pdf http://irep.iium.edu.my/61883/2/61883_Evaluation%20of%20Recellularization%20on%20Decellularized_SCOPUS.pdf
first_indexed	2023-09-18T21:27:47Z
last_indexed	2023-09-18T21:27:47Z
_version_	1777412308064010240

Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment

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