$Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves$

Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves

INTRODUCTION: Clinacanthus nutans is abundant in Malaysia. It contains bioactive compounds such as phenolics. PCA is a phenolic acid, naturally existed in plant species including C. nutans. It has beneficial effects especially for the treatment and prevention for diseases pertinent to oxidative stre...

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Main Authors:	Zamery, Muhammad 'Izzuddin, Ab. Hadi, Hazrina, Mohd Hassan, Norazian
Format:	Article
Language:	English English
Published:	Malaysian Pharmaceutical Society 2017
Subjects:	RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/61754/ http://irep.iium.edu.my/61754/ http://irep.iium.edu.my/61754/17/61754-Extraction%20of%20protocatechuic%20acid%20enriched.pdf http://irep.iium.edu.my/61754/2/Oral_MPSUSM%20presentation%20slide.pdf

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spelling	iium-617542018-02-06T03:23:42Z http://irep.iium.edu.my/61754/ Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves Zamery, Muhammad 'Izzuddin Ab. Hadi, Hazrina Mohd Hassan, Norazian RS192 Materia Medica-Pharmaceutical Technology INTRODUCTION: Clinacanthus nutans is abundant in Malaysia. It contains bioactive compounds such as phenolics. PCA is a phenolic acid, naturally existed in plant species including C. nutans. It has beneficial effects especially for the treatment and prevention for diseases pertinent to oxidative stress. OBJECTIVES: To extract PCA enriched fraction from C. nutans leaves, analyse it with validated high-performance liquid chromatography (HPLC) method, assess its anti-oxidant capacity. METHODS: The leaves were macerated in n-hexane followed by dichloromethane and methanol. Methanol extract was fractionated by vacuum liquid chromatography (VLC) and the fractions were screened by thin layer chromatography (TLC). The fraction was analysed by validated HPLC method. Anti-oxidant activity assay was performed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. RESULTS: 32 fractions were obtained with VLC. Four fractions (F1 – F4) were acquired after pooling similar TLC pattern. As compared to F3, F2 had been observed to possess more intense spot of PCA while F1 and F4 have no PCA spot. All parameters for HPLC method validation were within the specifications. HPLC of F2 showed PCA at 8.124 minutes retention time with the highest peak area among other compounds at 260 nm. Anti-oxidant assay showed anti-oxidant activity for F2 (IC50=898.51±10.93mg/mL) is the highest as compared to unfractionated methanol extract (IC50=1148.20±19.25mg/mL) (p=0.001) and other extracts (p<0.001). CONCLUSION: PCA enriched fraction (F2) was obtained from C. nutans leaves. F2 possesses the highest anti-oxidant among others, thus, it can be utilized in future research focuses on anti-oxidant applications. Malaysian Pharmaceutical Society 2017-11 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/61754/17/61754-Extraction%20of%20protocatechuic%20acid%20enriched.pdf application/pdf en http://irep.iium.edu.my/61754/2/Oral_MPSUSM%20presentation%20slide.pdf Zamery, Muhammad 'Izzuddin and Ab. Hadi, Hazrina and Mohd Hassan, Norazian (2017) Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves. Malaysian Journal of Pharmacy, 3 (1 (Supplement)). p. 23. ISSN 1675-3666 http://www.mps.org.my/index.cfm?&menuid=146
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology Zamery, Muhammad 'Izzuddin Ab. Hadi, Hazrina Mohd Hassan, Norazian Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves
description	INTRODUCTION: Clinacanthus nutans is abundant in Malaysia. It contains bioactive compounds such as phenolics. PCA is a phenolic acid, naturally existed in plant species including C. nutans. It has beneficial effects especially for the treatment and prevention for diseases pertinent to oxidative stress. OBJECTIVES: To extract PCA enriched fraction from C. nutans leaves, analyse it with validated high-performance liquid chromatography (HPLC) method, assess its anti-oxidant capacity. METHODS: The leaves were macerated in n-hexane followed by dichloromethane and methanol. Methanol extract was fractionated by vacuum liquid chromatography (VLC) and the fractions were screened by thin layer chromatography (TLC). The fraction was analysed by validated HPLC method. Anti-oxidant activity assay was performed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. RESULTS: 32 fractions were obtained with VLC. Four fractions (F1 – F4) were acquired after pooling similar TLC pattern. As compared to F3, F2 had been observed to possess more intense spot of PCA while F1 and F4 have no PCA spot. All parameters for HPLC method validation were within the specifications. HPLC of F2 showed PCA at 8.124 minutes retention time with the highest peak area among other compounds at 260 nm. Anti-oxidant assay showed anti-oxidant activity for F2 (IC50=898.51±10.93mg/mL) is the highest as compared to unfractionated methanol extract (IC50=1148.20±19.25mg/mL) (p=0.001) and other extracts (p<0.001). CONCLUSION: PCA enriched fraction (F2) was obtained from C. nutans leaves. F2 possesses the highest anti-oxidant among others, thus, it can be utilized in future research focuses on anti-oxidant applications.
format	Article
author	Zamery, Muhammad 'Izzuddin Ab. Hadi, Hazrina Mohd Hassan, Norazian
author_facet	Zamery, Muhammad 'Izzuddin Ab. Hadi, Hazrina Mohd Hassan, Norazian
author_sort	Zamery, Muhammad 'Izzuddin
title	Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves
title_short	Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves
title_full	Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves
title_fullStr	Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves
title_full_unstemmed	Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves
title_sort	extraction of protocatechuic acid enriched fraction from clinacanthus nutans leaves
publisher	Malaysian Pharmaceutical Society
publishDate	2017
url	http://irep.iium.edu.my/61754/ http://irep.iium.edu.my/61754/ http://irep.iium.edu.my/61754/17/61754-Extraction%20of%20protocatechuic%20acid%20enriched.pdf http://irep.iium.edu.my/61754/2/Oral_MPSUSM%20presentation%20slide.pdf
first_indexed	2023-09-18T21:27:34Z
last_indexed	2023-09-18T21:27:34Z
_version_	1777412294174572544

Extraction of protocatechuic acid enriched fraction from Clinacanthus nutans leaves

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