Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes

Objectives/Research Problem: Alternative traditional medicines and herbal remedies are continuously being used in the prevention of various diseases due to their beneficial health implications and costeffectiveness. One of these diseases is a state of hyperglycemia which can be managed through the...

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Main Authors:	Sassi, Assia, Khattak, Muhammad Muzaffar Ali Khan, Bakhtiar, M. Taher
Format:	Article
Language:	English
Published:	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2017
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/61736/ http://irep.iium.edu.my/61736/ http://irep.iium.edu.my/61736/1/61736_Enhancing%20Effects%20of%20Trichosanthes.pdf

id	iium-61736
recordtype	eprints
spelling	iium-617362018-02-19T06:47:41Z http://irep.iium.edu.my/61736/ Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher TX341 Nutrition. Foods and food supply Objectives/Research Problem: Alternative traditional medicines and herbal remedies are continuously being used in the prevention of various diseases due to their beneficial health implications and costeffectiveness. One of these diseases is a state of hyperglycemia which can be managed through the use of natural products originating from herbs, fruits or vegetables. One of the proclaimed and underutilized vegetables used in this study is a commonly consumed gourd belonging to the Cucurbitaceae family, named: Trichosanthes Cucumerina (known as Snake gourd or Labu Ular). Materials and Method: Water and ethanol extracts of the whole vegetable were assessed for cell viability which revealed that 3T3-L1 adipocytes maximum toleration concentration was 0.063 mg/ml. The extracts were further tested on adipocytes’ differentiation and showed a stimulation of lipid droplets formation during adipogenesis. Results and Discussion: The extracts significantly (p<0.001) increased glycerol concentrations during adipolysis with concentrations of 75.34±3.69 and 43.50±4.23 µg/ml for the ethanol extract (TCWe) and water extract (TCWw) of the whole vegetable, respectively. The extracts also significantly (p<0.001) promoted the uptake of glucose into the cells with values of 71.24 and 44.47% for the ethanol extract (TCWe) and the water extract (TCWw) of the whole vegetable, respectively. Conclusion: The present study showed that there is a beneficial effect of the extracts of Trichosanthes Cucumerina on adipogenesis, adipolysis and glucose uptake on 3T3-L1 adipocytes which can be a key factor in the prevention and treatment of non-communicable diseases such as diabetes, obesity and metabolic syndrome. Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61736/1/61736_Enhancing%20Effects%20of%20Trichosanthes.pdf Sassi, Assia and Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher (2017) Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes. International Journal of Allied Health Sciences, 1 (1). p. 1. E-ISSN 2600-8491 http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/85
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes
description	Objectives/Research Problem: Alternative traditional medicines and herbal remedies are continuously being used in the prevention of various diseases due to their beneficial health implications and costeffectiveness. One of these diseases is a state of hyperglycemia which can be managed through the use of natural products originating from herbs, fruits or vegetables. One of the proclaimed and underutilized vegetables used in this study is a commonly consumed gourd belonging to the Cucurbitaceae family, named: Trichosanthes Cucumerina (known as Snake gourd or Labu Ular). Materials and Method: Water and ethanol extracts of the whole vegetable were assessed for cell viability which revealed that 3T3-L1 adipocytes maximum toleration concentration was 0.063 mg/ml. The extracts were further tested on adipocytes’ differentiation and showed a stimulation of lipid droplets formation during adipogenesis. Results and Discussion: The extracts significantly (p<0.001) increased glycerol concentrations during adipolysis with concentrations of 75.34±3.69 and 43.50±4.23 µg/ml for the ethanol extract (TCWe) and water extract (TCWw) of the whole vegetable, respectively. The extracts also significantly (p<0.001) promoted the uptake of glucose into the cells with values of 71.24 and 44.47% for the ethanol extract (TCWe) and the water extract (TCWw) of the whole vegetable, respectively. Conclusion: The present study showed that there is a beneficial effect of the extracts of Trichosanthes Cucumerina on adipogenesis, adipolysis and glucose uptake on 3T3-L1 adipocytes which can be a key factor in the prevention and treatment of non-communicable diseases such as diabetes, obesity and metabolic syndrome.
format	Article
author	Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher
author_facet	Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher
author_sort	Sassi, Assia
title	Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes
title_short	Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes
title_full	Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes
title_fullStr	Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes
title_full_unstemmed	Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes
title_sort	enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3t3-l1 adipocytes
publisher	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/61736/ http://irep.iium.edu.my/61736/ http://irep.iium.edu.my/61736/1/61736_Enhancing%20Effects%20of%20Trichosanthes.pdf
first_indexed	2023-09-18T21:27:32Z
last_indexed	2023-09-18T21:27:32Z
_version_	1777412292406673408

Enhancing effects of trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake in 3T3-L1 adipocytes

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