Trimmed off adipocytes: a source of newly secreted adipokines

Given the beneficial roles of adipokines on body metabolism and its profound protective effects against metabolic disease, a better understanding of the regulation of adipokines secretion is very important. The objective of this study was to isolate, detect and quantify the total proteins in trimmed...

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Main Authors:	Azahari, Nuraniza, Khattak, Muhammad Muzaffar Ali Khan, Mohd Shukri, Nor Azwani, Abdul Rahman, Suzanah, Syed Osman Idid, Syed Zahir Idid, Phang, Ing Chia, Md Isa, Muhammad Lokman, Md Aris, Mohd Aznan, Bakhtiar, M. Taher
Format:	Article
Language:	English English
Published:	Mattioli 1885 S.p.A. 2019
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/61718/ http://irep.iium.edu.my/61718/ http://irep.iium.edu.my/61718/1/61718%20_Trimmed%20off%20adipocytes%20%20a%20source%20of%20newly%20secreted%20of%20adiponectin.pdf http://irep.iium.edu.my/61718/7/61718%20_Trimmed%20off%20adipocytes-%20A%20source%20of%20newly%20secreted%20adiponectin_Scopus.pdf

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spelling	iium-617182020-02-24T02:57:23Z http://irep.iium.edu.my/61718/ Trimmed off adipocytes: a source of newly secreted adipokines Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani Abdul Rahman, Suzanah Syed Osman Idid, Syed Zahir Idid Phang, Ing Chia Md Isa, Muhammad Lokman Md Aris, Mohd Aznan Bakhtiar, M. Taher TX341 Nutrition. Foods and food supply Given the beneficial roles of adipokines on body metabolism and its profound protective effects against metabolic disease, a better understanding of the regulation of adipokines secretion is very important. The objective of this study was to isolate, detect and quantify the total proteins in trimmed off abdominal adipose tissues from halal meat sources namely chicken, beef and lamb. Abdominal adipose tissues were isolated from the aforementioned sources and delipidation of the tissues were performed by chloroform/methanol extractions. Then, the protein concentration was determined by using Protein Assay Bicinchoninate Kit method. This was followed by quantification of the adipokines using ELISA kit assay. The experiment was conducted in triplicates and the results are presented as means ± SD. Results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded 0.10 gram ofprotein amount which was 1.0 % from total tissue mass. The protein concentration in adipose tissue of chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml respectively. The isolated adiponectin concentration in chicken, beef and lamb were 158 ng/ml± 0.05, 24 ± 0.05µg/ml and 37 ± 0.08 ng/ml respectively. These sources will bear further investigation on isolation and formulation for diabetic rat models in near future. Keywords: Adipose tissue, protein, adiponectin Mattioli 1885 S.p.A. 2019-05-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61718/1/61718%20_Trimmed%20off%20adipocytes%20%20a%20source%20of%20newly%20secreted%20of%20adiponectin.pdf application/pdf en http://irep.iium.edu.my/61718/7/61718%20_Trimmed%20off%20adipocytes-%20A%20source%20of%20newly%20secreted%20adiponectin_Scopus.pdf Azahari, Nuraniza and Khattak, Muhammad Muzaffar Ali Khan and Mohd Shukri, Nor Azwani and Abdul Rahman, Suzanah and Syed Osman Idid, Syed Zahir Idid and Phang, Ing Chia and Md Isa, Muhammad Lokman and Md Aris, Mohd Aznan and Bakhtiar, M. Taher (2019) Trimmed off adipocytes: a source of newly secreted adipokines. Progress in Nutrition, 21 (Supplement 1). pp. 80-85. E-ISSN 1129-8723 http://www.mattioli1885journals.com/index.php/progressinnutrition/index
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani Abdul Rahman, Suzanah Syed Osman Idid, Syed Zahir Idid Phang, Ing Chia Md Isa, Muhammad Lokman Md Aris, Mohd Aznan Bakhtiar, M. Taher Trimmed off adipocytes: a source of newly secreted adipokines
description	Given the beneficial roles of adipokines on body metabolism and its profound protective effects against metabolic disease, a better understanding of the regulation of adipokines secretion is very important. The objective of this study was to isolate, detect and quantify the total proteins in trimmed off abdominal adipose tissues from halal meat sources namely chicken, beef and lamb. Abdominal adipose tissues were isolated from the aforementioned sources and delipidation of the tissues were performed by chloroform/methanol extractions. Then, the protein concentration was determined by using Protein Assay Bicinchoninate Kit method. This was followed by quantification of the adipokines using ELISA kit assay. The experiment was conducted in triplicates and the results are presented as means ± SD. Results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded 0.10 gram ofprotein amount which was 1.0 % from total tissue mass. The protein concentration in adipose tissue of chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml respectively. The isolated adiponectin concentration in chicken, beef and lamb were 158 ng/ml± 0.05, 24 ± 0.05µg/ml and 37 ± 0.08 ng/ml respectively. These sources will bear further investigation on isolation and formulation for diabetic rat models in near future. Keywords: Adipose tissue, protein, adiponectin
format	Article
author	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani Abdul Rahman, Suzanah Syed Osman Idid, Syed Zahir Idid Phang, Ing Chia Md Isa, Muhammad Lokman Md Aris, Mohd Aznan Bakhtiar, M. Taher
author_facet	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani Abdul Rahman, Suzanah Syed Osman Idid, Syed Zahir Idid Phang, Ing Chia Md Isa, Muhammad Lokman Md Aris, Mohd Aznan Bakhtiar, M. Taher
author_sort	Azahari, Nuraniza
title	Trimmed off adipocytes: a source of newly secreted adipokines
title_short	Trimmed off adipocytes: a source of newly secreted adipokines
title_full	Trimmed off adipocytes: a source of newly secreted adipokines
title_fullStr	Trimmed off adipocytes: a source of newly secreted adipokines
title_full_unstemmed	Trimmed off adipocytes: a source of newly secreted adipokines
title_sort	trimmed off adipocytes: a source of newly secreted adipokines
publisher	Mattioli 1885 S.p.A.
publishDate	2019
url	http://irep.iium.edu.my/61718/ http://irep.iium.edu.my/61718/ http://irep.iium.edu.my/61718/1/61718%20_Trimmed%20off%20adipocytes%20%20a%20source%20of%20newly%20secreted%20of%20adiponectin.pdf http://irep.iium.edu.my/61718/7/61718%20_Trimmed%20off%20adipocytes-%20A%20source%20of%20newly%20secreted%20adiponectin_Scopus.pdf
first_indexed	2023-09-18T21:27:31Z
last_indexed	2023-09-18T21:27:31Z
_version_	1777412290977464320

Trimmed off adipocytes: a source of newly secreted adipokines

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