Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes

The aim of this study was to investigate the effect of bottle gourd which belong to family of cucurbitaceae (Lagenaria Siceraria (LS) on hyperglycemia by assessing the cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin using 3T3-L1 adipocytes as a model. Fresh bottle g...

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Main Authors:	Sassi, Assia, Khattak, Muhammad Muzaffar Ali Khan, Bakhtiar, M. Taher
Format:	Article
Language:	English English
Published:	MATTIOLI 1885 1029
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/1/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_article.pdf http://irep.iium.edu.my/61714/2/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_wos.pdf

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spelling	iium-617142020-03-14T03:55:15Z http://irep.iium.edu.my/61714/ Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher TX341 Nutrition. Foods and food supply The aim of this study was to investigate the effect of bottle gourd which belong to family of cucurbitaceae (Lagenaria Siceraria (LS) on hyperglycemia by assessing the cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin using 3T3-L1 adipocytes as a model. Fresh bottle gourd were washed thoroughly with distilled water and segregated into three parts i.e. whole vegetable (LSW), peels (LSP) and seeds (LSS) and were separately blended with water and ethanol. Thus obtained six types of extracts i.e. three for water whole vegetable (LSWw), peels (LSPw), seeds (LSSw) and three for ethanol whole vegetable (LSWe), peels (LSPe) and seeds (LSSe). The 3T3-L1 adipocytes were treated with these extracts for cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin. The collected data was compiled and statistical analysis were performed. Mainly, one-way analysis of variance (ANOVA) were performed followed by Tukey’s post-hoc test to ascertain differences in the means. The lipid droplet formation was significantly (P<0.001) higher in the adipocytes treated with the extracts of LSWw, LSSw, LSWe, LSWe, LSSe and lower in the extracts of LS peels (LSPw, LSPe) compared to induction medium (MDI)/insulin (control). The extracts also significantly (P<0.001) increased glycerol release during adipolysis compared to the control. It caused a significant (P<0.001) increase in adiponectin concentrations for LSPw, LSWw and a decrease in leptin concentrations for the water extracts of the LSPw. The present study showed that there was hypoglycaemic effect of LS extract by improving the regulation of adipogenesis, adipolysis, glucose uptake, adiponectin and leptin in the adipocytes mainly due to the seeds. MATTIOLI 1885 1029 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61714/1/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_article.pdf application/pdf en http://irep.iium.edu.my/61714/2/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_wos.pdf Sassi, Assia and Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher (1029) Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes. Progress in Nutrition, 21 (Supplement 2 (2019)). pp. 238-245. E-ISSN 1129-8723 https://mattioli1885journals.com/index.php/progressinnutrition/article/view/6367/8498 10.23751/pn.v21i2-S.6367
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes
description	The aim of this study was to investigate the effect of bottle gourd which belong to family of cucurbitaceae (Lagenaria Siceraria (LS) on hyperglycemia by assessing the cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin using 3T3-L1 adipocytes as a model. Fresh bottle gourd were washed thoroughly with distilled water and segregated into three parts i.e. whole vegetable (LSW), peels (LSP) and seeds (LSS) and were separately blended with water and ethanol. Thus obtained six types of extracts i.e. three for water whole vegetable (LSWw), peels (LSPw), seeds (LSSw) and three for ethanol whole vegetable (LSWe), peels (LSPe) and seeds (LSSe). The 3T3-L1 adipocytes were treated with these extracts for cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin. The collected data was compiled and statistical analysis were performed. Mainly, one-way analysis of variance (ANOVA) were performed followed by Tukey’s post-hoc test to ascertain differences in the means. The lipid droplet formation was significantly (P<0.001) higher in the adipocytes treated with the extracts of LSWw, LSSw, LSWe, LSWe, LSSe and lower in the extracts of LS peels (LSPw, LSPe) compared to induction medium (MDI)/insulin (control). The extracts also significantly (P<0.001) increased glycerol release during adipolysis compared to the control. It caused a significant (P<0.001) increase in adiponectin concentrations for LSPw, LSWw and a decrease in leptin concentrations for the water extracts of the LSPw. The present study showed that there was hypoglycaemic effect of LS extract by improving the regulation of adipogenesis, adipolysis, glucose uptake, adiponectin and leptin in the adipocytes mainly due to the seeds.
format	Article
author	Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher
author_facet	Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher
author_sort	Sassi, Assia
title	Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes
title_short	Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes
title_full	Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes
title_fullStr	Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes
title_full_unstemmed	Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes
title_sort	bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3t3-l1 adipocytes
publisher	MATTIOLI 1885
publishDate	1029
url	http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/1/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_article.pdf http://irep.iium.edu.my/61714/2/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_wos.pdf
first_indexed	2023-09-18T21:27:31Z
last_indexed	2023-09-18T21:27:31Z
_version_	1777412290679668736

Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes

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