Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model

Trichosanthes cucumerina (Cucurbitaceae) commonly known as Snake gourd or Labu Ular is considered the largest genre in the Cucurbitaceae family and is mainly found in the southeast areas of Asia. It has been used in Ayurvedic medicine as a treatment for certain diseases such as Diabetes mellitus,...

Full description

Bibliographic Details
Main Authors:	Sassi, Assia, Khattak, Muhammad Muzaffar Ali Khan, Bakhtiar, M. Taher
Format:	Article
Language:	English English
Published:	Rynnye Lyan Resources 2018
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/61710/ http://irep.iium.edu.my/61710/ http://irep.iium.edu.my/61710/ http://irep.iium.edu.my/61710/1/61710_Trichosanthes%20cucumerina%20extracts.pdf http://irep.iium.edu.my/61710/2/61710_Trichosanthes%20cucumerina%20extracts_SCOPUS.pdf

id	iium-61710
recordtype	eprints
spelling	iium-617102019-06-24T02:36:20Z http://irep.iium.edu.my/61710/ Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher TX341 Nutrition. Foods and food supply Trichosanthes cucumerina (Cucurbitaceae) commonly known as Snake gourd or Labu Ular is considered the largest genre in the Cucurbitaceae family and is mainly found in the southeast areas of Asia. It has been used in Ayurvedic medicine as a treatment for certain diseases such as Diabetes mellitus, but these acclaims lack scientific-based evidence. In this study, water and ethanol extracts of three parts of Trichosanthes cucumerina namely; whole vegetable, peels, and seeds, were assessed for toxicity through a cell viability assay using 3T3-L1 pre-adipocytes model which revealed a maximum toleration concentration of 0.063 mg/mL. The extracts were further tested on adipocytes’ differentiation and positively showed a stimulation of lipid droplets formation during adipogenesis and significantly (p<0.001) increased glycerol release levels (75.34±3.69 µg/ml) during adipolysis. The extracts also significantly (p<0.001) promoted the uptake of glucose into the cells (2636.22±91.33 Bq) in an action similar to that of insulin. Similar results were observed during ELISA assay with a significant increase (p<0.001) in adiponectin concentrations (3593.1±225.25 ng/mL) and a decrease in leptin concentrations (23870±5066.07 pg/mL). The present study results indicate a beneficial effect of Trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake, in addition to a regulation of adiponectin and leptin concentrations in 3T3-L1 adipocytes which can be of clinical importance in energy regulation which is a key factor in treating diabetes, obesity, and metabolic syndrome. Rynnye Lyan Resources 2018-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61710/1/61710_Trichosanthes%20cucumerina%20extracts.pdf application/pdf en http://irep.iium.edu.my/61710/2/61710_Trichosanthes%20cucumerina%20extracts_SCOPUS.pdf Sassi, Assia and Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher (2018) Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model. Food Research, 2 (2). pp. 146-153. E-ISSN 2550-2166 http://www.myfoodresearch.com/uploads/8/4/8/5/84855864/fr-2017-230.pdf 10.26656/fr.2017.2(2).230
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model
description	Trichosanthes cucumerina (Cucurbitaceae) commonly known as Snake gourd or Labu Ular is considered the largest genre in the Cucurbitaceae family and is mainly found in the southeast areas of Asia. It has been used in Ayurvedic medicine as a treatment for certain diseases such as Diabetes mellitus, but these acclaims lack scientific-based evidence. In this study, water and ethanol extracts of three parts of Trichosanthes cucumerina namely; whole vegetable, peels, and seeds, were assessed for toxicity through a cell viability assay using 3T3-L1 pre-adipocytes model which revealed a maximum toleration concentration of 0.063 mg/mL. The extracts were further tested on adipocytes’ differentiation and positively showed a stimulation of lipid droplets formation during adipogenesis and significantly (p<0.001) increased glycerol release levels (75.34±3.69 µg/ml) during adipolysis. The extracts also significantly (p<0.001) promoted the uptake of glucose into the cells (2636.22±91.33 Bq) in an action similar to that of insulin. Similar results were observed during ELISA assay with a significant increase (p<0.001) in adiponectin concentrations (3593.1±225.25 ng/mL) and a decrease in leptin concentrations (23870±5066.07 pg/mL). The present study results indicate a beneficial effect of Trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake, in addition to a regulation of adiponectin and leptin concentrations in 3T3-L1 adipocytes which can be of clinical importance in energy regulation which is a key factor in treating diabetes, obesity, and metabolic syndrome.
format	Article
author	Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher
author_facet	Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher
author_sort	Sassi, Assia
title	Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model
title_short	Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model
title_full	Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model
title_fullStr	Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model
title_full_unstemmed	Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model
title_sort	trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3t3-l1 adipocytes model
publisher	Rynnye Lyan Resources
publishDate	2018
url	http://irep.iium.edu.my/61710/ http://irep.iium.edu.my/61710/ http://irep.iium.edu.my/61710/ http://irep.iium.edu.my/61710/1/61710_Trichosanthes%20cucumerina%20extracts.pdf http://irep.iium.edu.my/61710/2/61710_Trichosanthes%20cucumerina%20extracts_SCOPUS.pdf
first_indexed	2023-09-18T21:27:30Z
last_indexed	2023-09-18T21:27:30Z
_version_	1777412290107146240

Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model

Similar Items