Trichosanthes cucumerina extracts enhance glucose uptake and regulate adiponectin and leptin concentrations in 3T3-L1 adipocytes model
Trichosanthes cucumerina (Cucurbitaceae) commonly known as Snake gourd or Labu Ular is considered the largest genre in the Cucurbitaceae family and is mainly found in the southeast areas of Asia. It has been used in Ayurvedic medicine as a treatment for certain diseases such as Diabetes mellitus,...
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| Format: | Article |
| Language: | English English |
| Published: |
Rynnye Lyan Resources
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/61710/ http://irep.iium.edu.my/61710/ http://irep.iium.edu.my/61710/ http://irep.iium.edu.my/61710/1/61710_Trichosanthes%20cucumerina%20extracts.pdf http://irep.iium.edu.my/61710/2/61710_Trichosanthes%20cucumerina%20extracts_SCOPUS.pdf |
| Summary: | Trichosanthes cucumerina (Cucurbitaceae) commonly known as Snake gourd or Labu
Ular is considered the largest genre in the Cucurbitaceae family and is mainly found in the
southeast areas of Asia. It has been used in Ayurvedic medicine as a treatment for certain
diseases such as Diabetes mellitus, but these acclaims lack scientific-based evidence. In
this study, water and ethanol extracts of three parts of Trichosanthes cucumerina namely;
whole vegetable, peels, and seeds, were assessed for toxicity through a cell viability assay
using 3T3-L1 pre-adipocytes model which revealed a maximum toleration concentration
of 0.063 mg/mL. The extracts were further tested on adipocytes’ differentiation and
positively showed a stimulation of lipid droplets formation during adipogenesis and
significantly (p<0.001) increased glycerol release levels (75.34±3.69 µg/ml) during
adipolysis. The extracts also significantly (p<0.001) promoted the uptake of glucose into
the cells (2636.22±91.33 Bq) in an action similar to that of insulin. Similar results were
observed during ELISA assay with a significant increase (p<0.001) in adiponectin
concentrations (3593.1±225.25 ng/mL) and a decrease in leptin concentrations
(23870±5066.07 pg/mL). The present study results indicate a beneficial effect of
Trichosanthes cucumerina extracts on adipogenesis, adipolysis and glucose uptake, in
addition to a regulation of adiponectin and leptin concentrations in 3T3-L1 adipocytes
which can be of clinical importance in energy regulation which is a key factor in treating
diabetes, obesity, and metabolic syndrome. |
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