PCB biodegration using bacteria isolated from landfill leachate

Polychlorinated biphenyls, PCBs are toxic, persistent organic pollutants (POP) which are harmful to human and environment due to their lipophilic characteristic and not easily degraded in the environment. Biodegradation is one of the alternative ways to reduce PCBs contamination. This study intend...

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Main Authors:	Nordin, Noor Faizul Hadry, Mohd Idris, Siti Nur Nadiah, Mohd Nawi, Mohd Nazri, John, Akbar, Chowdhury, Ahmed Jalal Khan
Format:	Article
Language:	English
Published:	RAZI Publishing 2017
Subjects:	Q Science (General) QL Zoology
Online Access:	http://irep.iium.edu.my/61458/ http://irep.iium.edu.my/61458/ http://irep.iium.edu.my/61458/1/74.pdf

id	iium-61458
recordtype	eprints
spelling	iium-614582018-01-17T09:01:22Z http://irep.iium.edu.my/61458/ PCB biodegration using bacteria isolated from landfill leachate Nordin, Noor Faizul Hadry Mohd Idris, Siti Nur Nadiah Mohd Nawi, Mohd Nazri John, Akbar Chowdhury, Ahmed Jalal Khan Q Science (General) QL Zoology Polychlorinated biphenyls, PCBs are toxic, persistent organic pollutants (POP) which are harmful to human and environment due to their lipophilic characteristic and not easily degraded in the environment. Biodegradation is one of the alternative ways to reduce PCBs contamination. This study intended to isolate bacteria from landfill leachate using enrichment culture technique and optimize the growth condition of selected isolates for degradation of PCBs. PCB biodegradation efficiency by selected bacterial isolates was also determined using GC-MS. Three potential PCBs degraders were isolated from the samples of landfill leachate through enrichment culture technique, which utilised PCBs as sole carbon and energy sources but only isolate T12B shown significant effect on the degradation. Three different growth parameters were optimized involving the temperature, pH, and PCBs concentration. Central composite design (CCD) from Design Expert Software was used to design the experiment. The optimal growth condition of temperature at 37˚C with 10 ppm of PCB concentrations and pH 8 for the culture medium was achieved using Response Surface Methodology (RSM) analysis. Based on GC-MS analysis, the reduction of PCBs concentration indicates that they were utilized by isolates T12B as sole of carbon and energy sources. This may suggest that bacteria in landfill leachate play an important role in biodegradation process as well as more effective and economical. RAZI Publishing 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61458/1/74.pdf Nordin, Noor Faizul Hadry and Mohd Idris, Siti Nur Nadiah and Mohd Nawi, Mohd Nazri and John, Akbar and Chowdhury, Ahmed Jalal Khan (2017) PCB biodegration using bacteria isolated from landfill leachate. Science Heritage Journal, 1 (2). pp. 7-10. ISSN 2521-0858 E-ISSN 2521-0866 https://zibelinepub.com/archives/2gws2017/2gws2017-07-10.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QL Zoology
spellingShingle	Q Science (General) QL Zoology Nordin, Noor Faizul Hadry Mohd Idris, Siti Nur Nadiah Mohd Nawi, Mohd Nazri John, Akbar Chowdhury, Ahmed Jalal Khan PCB biodegration using bacteria isolated from landfill leachate
description	Polychlorinated biphenyls, PCBs are toxic, persistent organic pollutants (POP) which are harmful to human and environment due to their lipophilic characteristic and not easily degraded in the environment. Biodegradation is one of the alternative ways to reduce PCBs contamination. This study intended to isolate bacteria from landfill leachate using enrichment culture technique and optimize the growth condition of selected isolates for degradation of PCBs. PCB biodegradation efficiency by selected bacterial isolates was also determined using GC-MS. Three potential PCBs degraders were isolated from the samples of landfill leachate through enrichment culture technique, which utilised PCBs as sole carbon and energy sources but only isolate T12B shown significant effect on the degradation. Three different growth parameters were optimized involving the temperature, pH, and PCBs concentration. Central composite design (CCD) from Design Expert Software was used to design the experiment. The optimal growth condition of temperature at 37˚C with 10 ppm of PCB concentrations and pH 8 for the culture medium was achieved using Response Surface Methodology (RSM) analysis. Based on GC-MS analysis, the reduction of PCBs concentration indicates that they were utilized by isolates T12B as sole of carbon and energy sources. This may suggest that bacteria in landfill leachate play an important role in biodegradation process as well as more effective and economical.
format	Article
author	Nordin, Noor Faizul Hadry Mohd Idris, Siti Nur Nadiah Mohd Nawi, Mohd Nazri John, Akbar Chowdhury, Ahmed Jalal Khan
author_facet	Nordin, Noor Faizul Hadry Mohd Idris, Siti Nur Nadiah Mohd Nawi, Mohd Nazri John, Akbar Chowdhury, Ahmed Jalal Khan
author_sort	Nordin, Noor Faizul Hadry
title	PCB biodegration using bacteria isolated from landfill leachate
title_short	PCB biodegration using bacteria isolated from landfill leachate
title_full	PCB biodegration using bacteria isolated from landfill leachate
title_fullStr	PCB biodegration using bacteria isolated from landfill leachate
title_full_unstemmed	PCB biodegration using bacteria isolated from landfill leachate
title_sort	pcb biodegration using bacteria isolated from landfill leachate
publisher	RAZI Publishing
publishDate	2017
url	http://irep.iium.edu.my/61458/ http://irep.iium.edu.my/61458/ http://irep.iium.edu.my/61458/1/74.pdf
first_indexed	2023-09-18T21:27:10Z
last_indexed	2023-09-18T21:27:10Z
_version_	1777412269222658048

PCB biodegration using bacteria isolated from landfill leachate

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