Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study

Treatment of osteomyelitis is still a major problem in orthopedic field. New Zealand White rabbit (NZWR) is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of this study wa...

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Main Authors:	Zulkifly, Ahmad Hafiz, Mohd Jan, Nurul Hafiza, Aziz, Aziah, Ibrahim, Mohd Zulfadzli
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	RD701 Orthopedics
Online Access:	http://irep.iium.edu.my/61457/ http://irep.iium.edu.my/61457/11/61457-Comparing%20Impregnated%20of%20Biomaterials%20with%20gentamicin.pdf

id	iium-61457
recordtype	eprints
spelling	iium-614572018-01-17T07:59:16Z http://irep.iium.edu.my/61457/ Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Aziz, Aziah Ibrahim, Mohd Zulfadzli RD701 Orthopedics Treatment of osteomyelitis is still a major problem in orthopedic field. New Zealand White rabbit (NZWR) is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of this study was to analyze the treatment given via impregnated biomaterials with gentamicin beads in rabbit models. Osteomyelitis was developed by inoculation of Staphylococcus aureus ATCC 25923 into the drilled rabbit’s distal femur. Thirty-two (32) NZWR was used during the research. The rabbits were divided into 2 groups; (hydroxyapatite (HA) and calcium sulphate (CaSO4)) with four subgroups assessments in (3, 6, 12 and 26) weeks. The responses of the treatments against infection were evaluated through gross presentations, radiograph, micro CT, microbiology and histology. The rabbits were sacrificed accordingly to evaluate the healing process of bone. Results showed osteomyelitis changes were noted in all rabbit after inoculation of bacteria at week 3 intervals. Intervention was done after 3 weeks of inoculation. Chronic osteomyelitis was confirmed via radiology and microbial assessments. SPSS Version 23 was used to evaluate the results. The microbiology analysis revealed there was no bacterium isolated at 26th week interval in both group. Histology interpretation, healing was noted at site with appearance of new bone formation at 6 to 26 weeks of intervals. The micro CT results showed that trabeculae numbers increased significantly (p<0.05) with the treatment. The results show bone healing at 26th week of interval. There was no significant difference between both treatments modalities in the area of radiology, histology and micro CT assessment (p<0.05). The impregnated antibiotics biomaterial have its function in the treatment of osteomyelitis. In conclusion, these result showed that impregnated biomaterials with gentamicin have roles in treatment of osteomyelitis as added to medical and surgical treatment. 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/61457/11/61457-Comparing%20Impregnated%20of%20Biomaterials%20with%20gentamicin.pdf Zulkifly, Ahmad Hafiz and Mohd Jan, Nurul Hafiza and Aziz, Aziah and Ibrahim, Mohd Zulfadzli (2017) Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study. In: Bio-CAM 2017 Conference on Biomedical & Advanced Material, 28th-29th November 2017, Langkawi, Kedah. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RD701 Orthopedics
spellingShingle	RD701 Orthopedics Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Aziz, Aziah Ibrahim, Mohd Zulfadzli Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study
description	Treatment of osteomyelitis is still a major problem in orthopedic field. New Zealand White rabbit (NZWR) is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of this study was to analyze the treatment given via impregnated biomaterials with gentamicin beads in rabbit models. Osteomyelitis was developed by inoculation of Staphylococcus aureus ATCC 25923 into the drilled rabbit’s distal femur. Thirty-two (32) NZWR was used during the research. The rabbits were divided into 2 groups; (hydroxyapatite (HA) and calcium sulphate (CaSO4)) with four subgroups assessments in (3, 6, 12 and 26) weeks. The responses of the treatments against infection were evaluated through gross presentations, radiograph, micro CT, microbiology and histology. The rabbits were sacrificed accordingly to evaluate the healing process of bone. Results showed osteomyelitis changes were noted in all rabbit after inoculation of bacteria at week 3 intervals. Intervention was done after 3 weeks of inoculation. Chronic osteomyelitis was confirmed via radiology and microbial assessments. SPSS Version 23 was used to evaluate the results. The microbiology analysis revealed there was no bacterium isolated at 26th week interval in both group. Histology interpretation, healing was noted at site with appearance of new bone formation at 6 to 26 weeks of intervals. The micro CT results showed that trabeculae numbers increased significantly (p<0.05) with the treatment. The results show bone healing at 26th week of interval. There was no significant difference between both treatments modalities in the area of radiology, histology and micro CT assessment (p<0.05). The impregnated antibiotics biomaterial have its function in the treatment of osteomyelitis. In conclusion, these result showed that impregnated biomaterials with gentamicin have roles in treatment of osteomyelitis as added to medical and surgical treatment.
format	Conference or Workshop Item
author	Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Aziz, Aziah Ibrahim, Mohd Zulfadzli
author_facet	Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Aziz, Aziah Ibrahim, Mohd Zulfadzli
author_sort	Zulkifly, Ahmad Hafiz
title	Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study
title_short	Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study
title_full	Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study
title_fullStr	Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study
title_full_unstemmed	Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study
title_sort	comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in new zealand white rabbit – animal study
publishDate	2017
url	http://irep.iium.edu.my/61457/ http://irep.iium.edu.my/61457/11/61457-Comparing%20Impregnated%20of%20Biomaterials%20with%20gentamicin.pdf
first_indexed	2023-09-18T21:27:10Z
last_indexed	2023-09-18T21:27:10Z
_version_	1777412269090537472

Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study

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