In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf

In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf

Tetracera macrophylla vall. (Dilleniaceae), has been a neglected species from Tetracera genus despite its usefulness in the traditional herbal medicines. T. macrophylla leaves crude ethanol (95%) extract (ETM) and its different fractions obtained through liquid-liquid partitioning strategy were scre...

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Main Authors: Opeyemi Roheem, Fatimah, Mat So'ad, Siti Zaiton, Ahmed, Qamar Uddin, Latip, Jalifah, Alhassan, Muhammad
Format: Conference or Workshop Item
Language:English
English
Published: 2017
Subjects:
Q Science (General)
QD Chemistry
Online Access:http://irep.iium.edu.my/61450/
http://irep.iium.edu.my/61450/14/61450-IN%20VITRO%20%CE%91-%20GLUCOSIDASE.pdf
http://irep.iium.edu.my/61450/2/fatimah%20presentation_UKM%20Conference%2022%20Nov%202017.pdf
id iium-61450
recordtype eprints
spelling iium-614502018-01-18T01:17:16Z http://irep.iium.edu.my/61450/ In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf Opeyemi Roheem, Fatimah Mat So'ad, Siti Zaiton Ahmed, Qamar Uddin Latip, Jalifah Alhassan, Muhammad Q Science (General) QD Chemistry Tetracera macrophylla vall. (Dilleniaceae), has been a neglected species from Tetracera genus despite its usefulness in the traditional herbal medicines. T. macrophylla leaves crude ethanol (95%) extract (ETM) and its different fractions obtained through liquid-liquid partitioning strategy were screened for their total phenolics and flavonoids contents and then investigated using in vitro models for their inhibitory activity on α-glucosidase. Free radical scavenging techniques including 2, 2diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-3-ethylbenzothiazine-6-sulfonic acid (ABTS) were used to examine the antioxidant capacity. ETM was found to be rich in both phenolic and flavonoid contents (417.90 ± 20.16, 5.86 ± 0.51 µg gallic acid (GA) and quercetin (QU) equivalent/mg dry weight) and exhibited good radical scavenging activity on DPPH and ABTS radicals ( 78.00% and 79.0%, respectively). Ethyl acetate fraction exhibited highest phenolic and flavonoid contents (206.34 ± 4.86, 14.01± 1.20 µg GA and QU equivalent/mg dry weight, respectively), and it was also observed to exhibit highest scavenging capacity on DPPH and ABTS radicals viz., 96.73%, 76.51% respectively. Both ETM and ethyl acetate fraction showed a promising and concentration-dependent α-glucosidase inhibitory activity. Bioassay guided silica gel column chromatography purification of ethyl-acetate fraction afforded a triterpenoid (12 mg), and a flavonoid (10 mg) characterized as betullinic acid and 5,7-dihydroxy-8-methoxy flavone based on spectroscopic analysis (IR, NMR). These compounds displayed promising radical scavenging activity against DPPH and ABTS radicals with moderate inhibitory activity on α-glucosidase enzymes. 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/61450/14/61450-IN%20VITRO%20%CE%91-%20GLUCOSIDASE.pdf application/pdf en http://irep.iium.edu.my/61450/2/fatimah%20presentation_UKM%20Conference%2022%20Nov%202017.pdf Opeyemi Roheem, Fatimah and Mat So'ad, Siti Zaiton and Ahmed, Qamar Uddin and Latip, Jalifah and Alhassan, Muhammad (2017) In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf. In: University Kebangsaan Malaysia-Universiti Riau, Indonesia-Universitas Islam Indonesia-Prince of Songkla University, Thailand Joint Seminar 2017 (UKM-UR-UII-PSU Joint Seminar 2017), 20th-22nd November 2017, Bangi, Selangor. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic Q Science (General)
QD Chemistry
spellingShingle Q Science (General)
QD Chemistry
Opeyemi Roheem, Fatimah
Mat So'ad, Siti Zaiton
Ahmed, Qamar Uddin
Latip, Jalifah
Alhassan, Muhammad
In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf
description Tetracera macrophylla vall. (Dilleniaceae), has been a neglected species from Tetracera genus despite its usefulness in the traditional herbal medicines. T. macrophylla leaves crude ethanol (95%) extract (ETM) and its different fractions obtained through liquid-liquid partitioning strategy were screened for their total phenolics and flavonoids contents and then investigated using in vitro models for their inhibitory activity on α-glucosidase. Free radical scavenging techniques including 2, 2diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-3-ethylbenzothiazine-6-sulfonic acid (ABTS) were used to examine the antioxidant capacity. ETM was found to be rich in both phenolic and flavonoid contents (417.90 ± 20.16, 5.86 ± 0.51 µg gallic acid (GA) and quercetin (QU) equivalent/mg dry weight) and exhibited good radical scavenging activity on DPPH and ABTS radicals ( 78.00% and 79.0%, respectively). Ethyl acetate fraction exhibited highest phenolic and flavonoid contents (206.34 ± 4.86, 14.01± 1.20 µg GA and QU equivalent/mg dry weight, respectively), and it was also observed to exhibit highest scavenging capacity on DPPH and ABTS radicals viz., 96.73%, 76.51% respectively. Both ETM and ethyl acetate fraction showed a promising and concentration-dependent α-glucosidase inhibitory activity. Bioassay guided silica gel column chromatography purification of ethyl-acetate fraction afforded a triterpenoid (12 mg), and a flavonoid (10 mg) characterized as betullinic acid and 5,7-dihydroxy-8-methoxy flavone based on spectroscopic analysis (IR, NMR). These compounds displayed promising radical scavenging activity against DPPH and ABTS radicals with moderate inhibitory activity on α-glucosidase enzymes.
format Conference or Workshop Item
author Opeyemi Roheem, Fatimah
Mat So'ad, Siti Zaiton
Ahmed, Qamar Uddin
Latip, Jalifah
Alhassan, Muhammad
author_facet Opeyemi Roheem, Fatimah
Mat So'ad, Siti Zaiton
Ahmed, Qamar Uddin
Latip, Jalifah
Alhassan, Muhammad
author_sort Opeyemi Roheem, Fatimah
title In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf
title_short In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf
title_full In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf
title_fullStr In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf
title_full_unstemmed In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf
title_sort in vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from tetracera macrophylla vall leaf
publishDate 2017
url http://irep.iium.edu.my/61450/
http://irep.iium.edu.my/61450/14/61450-IN%20VITRO%20%CE%91-%20GLUCOSIDASE.pdf
http://irep.iium.edu.my/61450/2/fatimah%20presentation_UKM%20Conference%2022%20Nov%202017.pdf
first_indexed 2023-09-18T21:27:09Z
last_indexed 2023-09-18T21:27:09Z
_version_ 1777412268225462272

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