Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer

Colorectal cancer (CRC) is one of the most common cancers worldwide that arise from successive accumulation of genetic and epigenetics alterations. It is a major cause of morbidity and mortality globally. It is highly curable if detected early, as the polyps can be removed before they divide and ove...

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Main Authors:	Mamat, Maizatul Akma, Zainuddin, Norafiza, A. Talib, Norlelawati, Hamdan, Asmah Hanim
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	QH426 Genetics RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/61357/ http://irep.iium.edu.my/61357/17/61357_Methylation-Specific%20PCR%20Assay%20for%20Quantification%20of%20DNA_complete.pdf

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recordtype	eprints
spelling	iium-613572018-02-12T02:15:29Z http://irep.iium.edu.my/61357/ Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer Mamat, Maizatul Akma Zainuddin, Norafiza A. Talib, Norlelawati Hamdan, Asmah Hanim QH426 Genetics RC0254 Neoplasms. Tumors. Oncology (including Cancer) Colorectal cancer (CRC) is one of the most common cancers worldwide that arise from successive accumulation of genetic and epigenetics alterations. It is a major cause of morbidity and mortality globally. It is highly curable if detected early, as the polyps can be removed before they divide and overgrow. Unremoved polyps may invade other parts of the body. Hence, earlier detection would significantly reduce the number of death due to this cancer. The discovery of aberrant DNA methylation in CpG islands of a number of genes might be one of the important pathways for CRC carcinogenesis. Therefore, the aim of our study was to examine the relationship of DNA methylation levels of SPG20 gene, one of the gene that is commonly epigenetically methylated in CRC, using genomic DNA derived from the tissues of patients with this cancer. The case control studies consisted of 29 cancerous tissues and 29 normal tissues taken from CRC patients. The extracted DNA was bisulfite converted and the percentage of methylation of the DNA samples were calculated based on their Cq values assayed using quantitative methylation-specific PCR (qMSP) procedure. We found that the percentage of SPG20 methylation showed a statistically significant difference in CRC samples as compared to normal tissues. Our results showed high level of SPG20 methylation in CRC tissue samples, thus suggesting the involvement of methylation as one of the mechanism in CRC pathogenesis. Hence, the identification of methylation level of SPG20 may serve as potential indicator in early detection of CRC and provide useful insights in better understanding of CRC progression. However, the finding of the study is limited due to small sample size,and evaluation at a larger scale involving other prevalent genes is necessary. 2017-11 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/61357/17/61357_Methylation-Specific%20PCR%20Assay%20for%20Quantification%20of%20DNA_complete.pdf Mamat, Maizatul Akma and Zainuddin, Norafiza and A. Talib, Norlelawati and Hamdan, Asmah Hanim (2017) Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer. In: 7th Regional Conference on Molecular Medicine (RCMM) in conjunction with 3rd National Conference for Cancer Research, 10-12 November 2017, UKM Medical Molecular Biology Institute, UKMMC, Cheras. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QH426 Genetics RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	QH426 Genetics RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mamat, Maizatul Akma Zainuddin, Norafiza A. Talib, Norlelawati Hamdan, Asmah Hanim Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer
description	Colorectal cancer (CRC) is one of the most common cancers worldwide that arise from successive accumulation of genetic and epigenetics alterations. It is a major cause of morbidity and mortality globally. It is highly curable if detected early, as the polyps can be removed before they divide and overgrow. Unremoved polyps may invade other parts of the body. Hence, earlier detection would significantly reduce the number of death due to this cancer. The discovery of aberrant DNA methylation in CpG islands of a number of genes might be one of the important pathways for CRC carcinogenesis. Therefore, the aim of our study was to examine the relationship of DNA methylation levels of SPG20 gene, one of the gene that is commonly epigenetically methylated in CRC, using genomic DNA derived from the tissues of patients with this cancer. The case control studies consisted of 29 cancerous tissues and 29 normal tissues taken from CRC patients. The extracted DNA was bisulfite converted and the percentage of methylation of the DNA samples were calculated based on their Cq values assayed using quantitative methylation-specific PCR (qMSP) procedure. We found that the percentage of SPG20 methylation showed a statistically significant difference in CRC samples as compared to normal tissues. Our results showed high level of SPG20 methylation in CRC tissue samples, thus suggesting the involvement of methylation as one of the mechanism in CRC pathogenesis. Hence, the identification of methylation level of SPG20 may serve as potential indicator in early detection of CRC and provide useful insights in better understanding of CRC progression. However, the finding of the study is limited due to small sample size,and evaluation at a larger scale involving other prevalent genes is necessary.
format	Conference or Workshop Item
author	Mamat, Maizatul Akma Zainuddin, Norafiza A. Talib, Norlelawati Hamdan, Asmah Hanim
author_facet	Mamat, Maizatul Akma Zainuddin, Norafiza A. Talib, Norlelawati Hamdan, Asmah Hanim
author_sort	Mamat, Maizatul Akma
title	Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer
title_short	Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer
title_full	Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer
title_fullStr	Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer
title_full_unstemmed	Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer
title_sort	methylation-sppecific pcr assay for quantification of dna methylation of spg20 gene in colorectal cancer
publishDate	2017
url	http://irep.iium.edu.my/61357/ http://irep.iium.edu.my/61357/17/61357_Methylation-Specific%20PCR%20Assay%20for%20Quantification%20of%20DNA_complete.pdf
first_indexed	2023-09-18T21:27:02Z
last_indexed	2023-09-18T21:27:02Z
_version_	1777412260115775488

Methylation-sppecific PCR assay for quantification of DNA methylation of SPG20 gene in colorectal cancer

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