Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)

Natural products from plant have been used for centuries to prevent various diseases including cancer. Many chemopreventive agents are believed to inhibit or delay the progression of cancer cells through suppression of cell proliferation, induction of apoptosis and cell cycle arrest. In Malaysia, b...

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Main Authors:	Hassan, Habibah, Razali, Shakirah, Firus Khan, Al'aina Yuhainis, K Jalal, Tara, Salleh, Azil, Khatib, Alfi, Hashim, Ridzwan, Abdul Wahab, Ridhwan
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	SF Animal culture
Online Access:	http://irep.iium.edu.my/61123/ http://irep.iium.edu.my/61123/6/61123_Cancer%20Chemoprevention%20Study%20of%20Luffa%20Aegyptia_complete.pdf

id	iium-61123
recordtype	eprints
spelling	iium-611232018-01-23T06:56:21Z http://irep.iium.edu.my/61123/ Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7) Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina Yuhainis K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan SF Animal culture Natural products from plant have been used for centuries to prevent various diseases including cancer. Many chemopreventive agents are believed to inhibit or delay the progression of cancer cells through suppression of cell proliferation, induction of apoptosis and cell cycle arrest. In Malaysia, breast cancer is the most common form of cancer affecting women. The prognosis for breast cancer is still poor and conventional treatment is not always effective. Therefore, this study aims to determine the chemopreventive effect of Luffa aegyptiaca seed extract (LSE) on breast cancer through anti-proliferative effect and its mechanisms on breast cancer cell (MCF-7) as evidenced by the result of IC50 determination, cell proliferation assay and flow cytometer analysis on apoptosis induction and cell cycle arrest. MCF-7 cells were plated at a density of 4.7 x 104 cells/ml in 6–well plate. Trypan blue exclusion method was used to determine cell viability. Untreated and LSE-treated MCF-7 cells were observed for morphological changes with an inverted-phase contrast microscope. The induction of apoptosis and cell cycle arrest was analysed using flow cytometry. LSE showed growth inhibitory effect with IC50 value at 0.0625 mg/ml. Moreover, LSE, show no toxic effect on normal cells possibly showing that it is a potential chemopreventive agent on MCF-7 via the induction of apoptosis and cell cycle arrest. Overall, the data collected provides new insight for the use of Luffa aegyptiaca seed as a chmopreventive agent on MCF-7. Keywords: Luffa aegyptiaca; anti-proliferative effect; apoptosis; cell cycle; MCF-7 2016-12 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/61123/6/61123_Cancer%20Chemoprevention%20Study%20of%20Luffa%20Aegyptia_complete.pdf Hassan, Habibah and Razali, Shakirah and Firus Khan, Al'aina Yuhainis and K Jalal, Tara and Salleh, Azil and Khatib, Alfi and Hashim, Ridzwan and Abdul Wahab, Ridhwan (2016) Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7). In: 3rd Pan Asian Biomedical Science Conference 2016, 7-8th December 2016, Management and Science University (MSU), in Shah Alam, Selangor Darul Ehsan, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SF Animal culture
spellingShingle	SF Animal culture Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina Yuhainis K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)
description	Natural products from plant have been used for centuries to prevent various diseases including cancer. Many chemopreventive agents are believed to inhibit or delay the progression of cancer cells through suppression of cell proliferation, induction of apoptosis and cell cycle arrest. In Malaysia, breast cancer is the most common form of cancer affecting women. The prognosis for breast cancer is still poor and conventional treatment is not always effective. Therefore, this study aims to determine the chemopreventive effect of Luffa aegyptiaca seed extract (LSE) on breast cancer through anti-proliferative effect and its mechanisms on breast cancer cell (MCF-7) as evidenced by the result of IC50 determination, cell proliferation assay and flow cytometer analysis on apoptosis induction and cell cycle arrest. MCF-7 cells were plated at a density of 4.7 x 104 cells/ml in 6–well plate. Trypan blue exclusion method was used to determine cell viability. Untreated and LSE-treated MCF-7 cells were observed for morphological changes with an inverted-phase contrast microscope. The induction of apoptosis and cell cycle arrest was analysed using flow cytometry. LSE showed growth inhibitory effect with IC50 value at 0.0625 mg/ml. Moreover, LSE, show no toxic effect on normal cells possibly showing that it is a potential chemopreventive agent on MCF-7 via the induction of apoptosis and cell cycle arrest. Overall, the data collected provides new insight for the use of Luffa aegyptiaca seed as a chmopreventive agent on MCF-7. Keywords: Luffa aegyptiaca; anti-proliferative effect; apoptosis; cell cycle; MCF-7
format	Conference or Workshop Item
author	Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina Yuhainis K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan
author_facet	Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina Yuhainis K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan
author_sort	Hassan, Habibah
title	Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)
title_short	Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)
title_full	Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)
title_fullStr	Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)
title_full_unstemmed	Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)
title_sort	cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (mcf-7)
publishDate	2016
url	http://irep.iium.edu.my/61123/ http://irep.iium.edu.my/61123/6/61123_Cancer%20Chemoprevention%20Study%20of%20Luffa%20Aegyptia_complete.pdf
first_indexed	2023-09-18T21:26:41Z
last_indexed	2023-09-18T21:26:41Z
_version_	1777412238731116544

Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)

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