Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell

Natural products have been used in treating illnesses since ancient times. Neolamarckia cadamba (N. cadamba) plant, locally known as Kalempayan, is an Ayurvedic medicine. It has been used by Indian folklore for treatment such as fever, anaemia, stomatitis, and infectious diseases. This Rubiaceae fam...

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Main Authors:	Razali, Shakirah, Hassan, Habibah, Firus Khan, Al'aina Yuhainis, K Jalal, Tara, Abdul Jamil, Muhammad Mahadi, Johan, Muhammad Farid, Mohammad, Mardhiah, Draman, Samsul, Salleh, Azir, Khatib, Alfi, Hashim, Ridzwan, Abdul Wahab, Ridhwan
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	QH301 Biology
Online Access:	http://irep.iium.edu.my/61120/ http://irep.iium.edu.my/61120/1/PABSC%282%29.pdf

id	iium-61120
recordtype	eprints
spelling	iium-611202018-01-22T07:22:16Z http://irep.iium.edu.my/61120/ Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell Razali, Shakirah Hassan, Habibah Firus Khan, Al'aina Yuhainis K Jalal, Tara Abdul Jamil, Muhammad Mahadi Johan, Muhammad Farid Mohammad, Mardhiah Draman, Samsul Salleh, Azir Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan QH301 Biology Natural products have been used in treating illnesses since ancient times. Neolamarckia cadamba (N. cadamba) plant, locally known as Kalempayan, is an Ayurvedic medicine. It has been used by Indian folklore for treatment such as fever, anaemia, stomatitis, and infectious diseases. This Rubiaceae family plant indicates that it has a therapeutic potential against diseases, including cancer. Therefore, this study aimed to investigate the chemopreventive effect of N. cadamba’s leaves extract (NCE) with regard to the induction of apoptosis and cell cycle arrest against human breast adenocarcinoma cancer cell-line (MCF-7). MCF-7 cells were seeded at 4.7 x 104 cells/well in 6-well plates. The cells were treated with series concentration of NCE. The 50% inhibitory concentration (IC50) of NCE and its antiproliferative effect were measured using Trypan blue exclusion method was determined as 0.125 mg/ml. The flow cytometric analysis was performed in MCF-7 revealed the NCE inhibited the cell proliferation through induction of apoptosis and cell cycle arrest. The present study demonstrates that the NCE has a positive potential as a chemopreventive agent. Further research need to be done to identify the molecular mechanism related to induction of apoptosis and cell cycle arrest in breast cancer cells. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/61120/1/PABSC%282%29.pdf Razali, Shakirah and Hassan, Habibah and Firus Khan, Al'aina Yuhainis and K Jalal, Tara and Abdul Jamil, Muhammad Mahadi and Johan, Muhammad Farid and Mohammad, Mardhiah and Draman, Samsul and Salleh, Azir and Khatib, Alfi and Hashim, Ridzwan and Abdul Wahab, Ridhwan (2016) Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell. In: 3rd Pan-Asian Biomedical Science Conference 2016 (3rd PABSC), 7th–8th December 2016, Shah Alam, Selangor. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QH301 Biology
spellingShingle	QH301 Biology Razali, Shakirah Hassan, Habibah Firus Khan, Al'aina Yuhainis K Jalal, Tara Abdul Jamil, Muhammad Mahadi Johan, Muhammad Farid Mohammad, Mardhiah Draman, Samsul Salleh, Azir Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell
description	Natural products have been used in treating illnesses since ancient times. Neolamarckia cadamba (N. cadamba) plant, locally known as Kalempayan, is an Ayurvedic medicine. It has been used by Indian folklore for treatment such as fever, anaemia, stomatitis, and infectious diseases. This Rubiaceae family plant indicates that it has a therapeutic potential against diseases, including cancer. Therefore, this study aimed to investigate the chemopreventive effect of N. cadamba’s leaves extract (NCE) with regard to the induction of apoptosis and cell cycle arrest against human breast adenocarcinoma cancer cell-line (MCF-7). MCF-7 cells were seeded at 4.7 x 104 cells/well in 6-well plates. The cells were treated with series concentration of NCE. The 50% inhibitory concentration (IC50) of NCE and its antiproliferative effect were measured using Trypan blue exclusion method was determined as 0.125 mg/ml. The flow cytometric analysis was performed in MCF-7 revealed the NCE inhibited the cell proliferation through induction of apoptosis and cell cycle arrest. The present study demonstrates that the NCE has a positive potential as a chemopreventive agent. Further research need to be done to identify the molecular mechanism related to induction of apoptosis and cell cycle arrest in breast cancer cells.
format	Conference or Workshop Item
author	Razali, Shakirah Hassan, Habibah Firus Khan, Al'aina Yuhainis K Jalal, Tara Abdul Jamil, Muhammad Mahadi Johan, Muhammad Farid Mohammad, Mardhiah Draman, Samsul Salleh, Azir Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan
author_facet	Razali, Shakirah Hassan, Habibah Firus Khan, Al'aina Yuhainis K Jalal, Tara Abdul Jamil, Muhammad Mahadi Johan, Muhammad Farid Mohammad, Mardhiah Draman, Samsul Salleh, Azir Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan
author_sort	Razali, Shakirah
title	Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell
title_short	Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell
title_full	Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell
title_fullStr	Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell
title_full_unstemmed	Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell
title_sort	understanding of anti-cancer properties of neolamarckia cadamba (kalempayan) leaves extract on breast cancer cell
publishDate	2016
url	http://irep.iium.edu.my/61120/ http://irep.iium.edu.my/61120/1/PABSC%282%29.pdf
first_indexed	2023-09-18T21:26:41Z
last_indexed	2023-09-18T21:26:41Z
_version_	1777412238302248960

Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell

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