Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya

Introduction: Leaves of mempelas paya (Tetracera indica) (family: Dilleniaceae) is traditionally used in diabetes management in Malaysia. However, no attempt has ever been made to discover an active principle responsible for its blood glucose lowering effect. Hence, this research deals with the isol...

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Main Authors:	Ahmed, Qamar Uddin, Mat So'ad, Siti Zaiton, Afrin, Mehnaz, Wan Sulaiman, Wan Mohd Azizi
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Medicine, International Islamic University Malaysia 2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/61093/ http://irep.iium.edu.my/61093/17/61093_Investigation%20of%20an%20antidiabetic%20potential_complete.pdf

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recordtype	eprints
spelling	iium-610932019-01-02T04:05:01Z http://irep.iium.edu.my/61093/ Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Afrin, Mehnaz Wan Sulaiman, Wan Mohd Azizi QD Chemistry Introduction: Leaves of mempelas paya (Tetracera indica) (family: Dilleniaceae) is traditionally used in diabetes management in Malaysia. However, no attempt has ever been made to discover an active principle responsible for its blood glucose lowering effect. Hence, this research deals with the isolation, in vivo antidiabetic and toxicological evaluation of an active principle. Methods: Chemical investigation of methanol extract derived from the powdered leaves resulted in the isolation of a phenolic compound through repeated silica gel column chromatography. The structure was elucidated by spectroscopic (1H-NMR, 13C-NMR, MS, IR and UV) analysis. In vivo antidiabetic and toxicological studies were carried out using Sprague-Dawley rats (diabetic and normal) at three different concentrations (1, 5 and 25 mg/kg b.w.). Results: 5,7-dihydroxy-8-methoxyflavone was found to exhibit significant anti-hyperglycaemic activity (p<0.05) in diabetic rats at 5 and 25 mg/kg b.w. without showing hypoglycaemic effect in normal rats. The anti-hyperglycaemic activity was found to be comparable with glibenclamide (GLBC). In vivo toxicological study for continuous 21 days showed no abnormal activity and mortality. Histopathology of kidney, liver and pancreas of both normal and diabetic treated rats demonstrated normal and improved condition when compared to diabetic control group. There were no significant changes seen in the kidney (serum urea and creatinine) and lipid (TC, TG, LDL-C and HDL-C) functional parameters tested. Conclusions: 5,7-dihydroxy-8-methoxyflavone isolated from the leaves of mempelas paya coud be a safe antidiabetic agent. KEYWORDS: Tetracera indica, mempelas paya, 5,7-dihydroxy-8-methoxyflavone, in vivo, antidiabetic activity, toxicological study, histopathology Kulliyyah of Medicine, International Islamic University Malaysia 2016-08-04 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/61093/17/61093_Investigation%20of%20an%20antidiabetic%20potential_complete.pdf Ahmed, Qamar Uddin and Mat So'ad, Siti Zaiton and Afrin, Mehnaz and Wan Sulaiman, Wan Mohd Azizi (2016) Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 4 August 2016, Kulliyyah of Medicine, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Afrin, Mehnaz Wan Sulaiman, Wan Mohd Azizi Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
description	Introduction: Leaves of mempelas paya (Tetracera indica) (family: Dilleniaceae) is traditionally used in diabetes management in Malaysia. However, no attempt has ever been made to discover an active principle responsible for its blood glucose lowering effect. Hence, this research deals with the isolation, in vivo antidiabetic and toxicological evaluation of an active principle. Methods: Chemical investigation of methanol extract derived from the powdered leaves resulted in the isolation of a phenolic compound through repeated silica gel column chromatography. The structure was elucidated by spectroscopic (1H-NMR, 13C-NMR, MS, IR and UV) analysis. In vivo antidiabetic and toxicological studies were carried out using Sprague-Dawley rats (diabetic and normal) at three different concentrations (1, 5 and 25 mg/kg b.w.). Results: 5,7-dihydroxy-8-methoxyflavone was found to exhibit significant anti-hyperglycaemic activity (p<0.05) in diabetic rats at 5 and 25 mg/kg b.w. without showing hypoglycaemic effect in normal rats. The anti-hyperglycaemic activity was found to be comparable with glibenclamide (GLBC). In vivo toxicological study for continuous 21 days showed no abnormal activity and mortality. Histopathology of kidney, liver and pancreas of both normal and diabetic treated rats demonstrated normal and improved condition when compared to diabetic control group. There were no significant changes seen in the kidney (serum urea and creatinine) and lipid (TC, TG, LDL-C and HDL-C) functional parameters tested. Conclusions: 5,7-dihydroxy-8-methoxyflavone isolated from the leaves of mempelas paya coud be a safe antidiabetic agent. KEYWORDS: Tetracera indica, mempelas paya, 5,7-dihydroxy-8-methoxyflavone, in vivo, antidiabetic activity, toxicological study, histopathology
format	Conference or Workshop Item
author	Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Afrin, Mehnaz Wan Sulaiman, Wan Mohd Azizi
author_facet	Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Afrin, Mehnaz Wan Sulaiman, Wan Mohd Azizi
author_sort	Ahmed, Qamar Uddin
title	Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_short	Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_full	Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_fullStr	Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_full_unstemmed	Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_sort	investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
publisher	Kulliyyah of Medicine, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/61093/ http://irep.iium.edu.my/61093/17/61093_Investigation%20of%20an%20antidiabetic%20potential_complete.pdf
first_indexed	2023-09-18T21:26:38Z
last_indexed	2023-09-18T21:26:38Z
_version_	1777412235358896128

Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya

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