Universal mini COI barcode for the identification of fish species in processed products

Species substitution, the use of a low value fish in place of a high value fish, is the biggest problem in inter- national trade and the leading cause of fraud in the fisheries arena sector. Current DNA barcoding systems have partly solved this problem but also failed in many instances to amplify P...

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Main Authors:	Sultana, Sharmin, Ali, Md. Eaqub, Hossain, M.A. Motalib, ., Asing, Naquiah, Nina, Sarker, Md. Zaidul Islam
Format:	Article
Language:	English English English
Published:	Elsevier 2018
Subjects:	QD Chemistry TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/60938/ http://irep.iium.edu.my/60938/ http://irep.iium.edu.my/60938/ http://irep.iium.edu.my/60938/7/60938-Universal%20mini%20COI%20barcode%20for%20the%20identification%20of%20fish%20species-SCOPUS.pdf http://irep.iium.edu.my/60938/13/60938_Universal%20mini%20COI%20barcode%20for%20the%20identification.pdf http://irep.iium.edu.my/60938/14/60938_Universal%20mini%20COI%20barcode%20for%20the%20identification_WOS.pdf

id	iium-60938
recordtype	eprints
spelling	iium-609382018-07-10T00:42:56Z http://irep.iium.edu.my/60938/ Universal mini COI barcode for the identification of fish species in processed products Sultana, Sharmin Ali, Md. Eaqub Hossain, M.A. Motalib ., Asing Naquiah, Nina Sarker, Md. Zaidul Islam QD Chemistry TX341 Nutrition. Foods and food supply Species substitution, the use of a low value fish in place of a high value fish, is the biggest problem in inter- national trade and the leading cause of fraud in the fisheries arena sector. Current DNA barcoding systems have partly solved this problem but also failed in many instances to amplify PCR targets from highly processed products because of the degradation of a longer barcode marker (~ 650 bp). In the present study, a novel mini barcode marker (295 bp) was developed to discriminate fish species in raw and processed states forms. The barcode primers were cross-tested against 33 fish species and 15 other animal species and found to be universal for all the tested fish varieties. When 20 commercial fish products of five different categories were screened, all commercial fish sample yielded positive bands for the novel fish barcode. PCR product was sequenced to retrieve the species IDs that reflected 55% (11/20) of Malaysian fish products were mislabeled. Elsevier 2018-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60938/7/60938-Universal%20mini%20COI%20barcode%20for%20the%20identification%20of%20fish%20species-SCOPUS.pdf application/pdf en http://irep.iium.edu.my/60938/13/60938_Universal%20mini%20COI%20barcode%20for%20the%20identification.pdf application/pdf en http://irep.iium.edu.my/60938/14/60938_Universal%20mini%20COI%20barcode%20for%20the%20identification_WOS.pdf Sultana, Sharmin and Ali, Md. Eaqub and Hossain, M.A. Motalib and ., Asing and Naquiah, Nina and Sarker, Md. Zaidul Islam (2018) Universal mini COI barcode for the identification of fish species in processed products. Food Research International, 105. pp. 19-28. ISSN 0963-9969 https://www.sciencedirect.com/science/article/pii/S0963996917307500 10.1016/j.foodres.2017.10.065
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QD Chemistry TX341 Nutrition. Foods and food supply
spellingShingle	QD Chemistry TX341 Nutrition. Foods and food supply Sultana, Sharmin Ali, Md. Eaqub Hossain, M.A. Motalib ., Asing Naquiah, Nina Sarker, Md. Zaidul Islam Universal mini COI barcode for the identification of fish species in processed products
description	Species substitution, the use of a low value fish in place of a high value fish, is the biggest problem in inter- national trade and the leading cause of fraud in the fisheries arena sector. Current DNA barcoding systems have partly solved this problem but also failed in many instances to amplify PCR targets from highly processed products because of the degradation of a longer barcode marker (~ 650 bp). In the present study, a novel mini barcode marker (295 bp) was developed to discriminate fish species in raw and processed states forms. The barcode primers were cross-tested against 33 fish species and 15 other animal species and found to be universal for all the tested fish varieties. When 20 commercial fish products of five different categories were screened, all commercial fish sample yielded positive bands for the novel fish barcode. PCR product was sequenced to retrieve the species IDs that reflected 55% (11/20) of Malaysian fish products were mislabeled.
format	Article
author	Sultana, Sharmin Ali, Md. Eaqub Hossain, M.A. Motalib ., Asing Naquiah, Nina Sarker, Md. Zaidul Islam
author_facet	Sultana, Sharmin Ali, Md. Eaqub Hossain, M.A. Motalib ., Asing Naquiah, Nina Sarker, Md. Zaidul Islam
author_sort	Sultana, Sharmin
title	Universal mini COI barcode for the identification of fish species in processed products
title_short	Universal mini COI barcode for the identification of fish species in processed products
title_full	Universal mini COI barcode for the identification of fish species in processed products
title_fullStr	Universal mini COI barcode for the identification of fish species in processed products
title_full_unstemmed	Universal mini COI barcode for the identification of fish species in processed products
title_sort	universal mini coi barcode for the identification of fish species in processed products
publisher	Elsevier
publishDate	2018
url	http://irep.iium.edu.my/60938/ http://irep.iium.edu.my/60938/ http://irep.iium.edu.my/60938/ http://irep.iium.edu.my/60938/7/60938-Universal%20mini%20COI%20barcode%20for%20the%20identification%20of%20fish%20species-SCOPUS.pdf http://irep.iium.edu.my/60938/13/60938_Universal%20mini%20COI%20barcode%20for%20the%20identification.pdf http://irep.iium.edu.my/60938/14/60938_Universal%20mini%20COI%20barcode%20for%20the%20identification_WOS.pdf
first_indexed	2023-09-18T21:26:24Z
last_indexed	2023-09-18T21:26:24Z
_version_	1777412221083582464

Universal mini COI barcode for the identification of fish species in processed products

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