Optimization of process condition for extraction of gelatin from red tilapia skin (Oreochromis niloticus) by High Pressure Processing (HPP)

Extraction of gelatin using traditional acid-base pretreatment method has several limitations such as time consuming and causes serious water pollution. Chemical treatment often being used as an alternative process to overcome the weaknesses of the conventional method. However, excessive chemical...

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Main Authors: Yusof, N., Jaswir, Irwandi, Jamal, Parveen, Jami, Mohammed Saedi, Octavianti, Fitri
Format: Article
Language:English
Published: Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017
Subjects:
Online Access:http://irep.iium.edu.my/60862/
http://irep.iium.edu.my/60862/
http://irep.iium.edu.my/60862/1/60862_Optimization%20of%20process%20condition%20for%20extraction.pdf
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spelling iium-608622018-03-13T07:14:54Z http://irep.iium.edu.my/60862/ Optimization of process condition for extraction of gelatin from red tilapia skin (Oreochromis niloticus) by High Pressure Processing (HPP) Yusof, N. Jaswir, Irwandi Jamal, Parveen Jami, Mohammed Saedi Octavianti, Fitri QD Chemistry SH151 Aquaculture - Fish Culture Extraction of gelatin using traditional acid-base pretreatment method has several limitations such as time consuming and causes serious water pollution. Chemical treatment often being used as an alternative process to overcome the weaknesses of the conventional method. However, excessive chemical elements would damage the structure of the gelatin due to its high sensitivity to the acid content. High Pressure Processing (HPP) is a novel and environmental friendly method that has been suggested to assist gelatin extraction. Pressurization during pretreatment could reduce the extraction time and amount of acid used. It also has a potential in enhancing the properties of the gelatin extract and increasing the gelatin yield. In this research, One-Factorat-Time (OFAT) and optimization study were done to determine the optimum parameters for extraction of gelatin assisted by HPP from red tilapia skin. Four parameters; applied pressure, pressure holding time, ratio of acid to skin and extraction time have been selected for the OFAT design and concentration of the gelatin extract and percentage of yield gelatin were evaluated. From OFAT, optimum technical parameters for response surface optimization design were 250 MPa pressure, 7.5 ml of acid to 1 g of skin and 12 hours extraction time. Pressure holding time was fixed for 10 min. FCCCD has been used for optimization study. Results from the data shows that the optimum conditions for gelatin extraction from red tilapia skin were 250 MPa for pressure, 10 min of pressure holding time, 7.5 ml of acid for 1 g of skin and 12 hours of extraction time while the maximum concentration and yield were 19.51 mg/ml and 32.04% (320.4 mg/g), respectively. These findings proved that HPP could increase the concentration and the yield of the gelatin while reducing the chemical waste and shortening the extraction process. Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60862/1/60862_Optimization%20of%20process%20condition%20for%20extraction.pdf Yusof, N. and Jaswir, Irwandi and Jamal, Parveen and Jami, Mohammed Saedi and Octavianti, Fitri (2017) Optimization of process condition for extraction of gelatin from red tilapia skin (Oreochromis niloticus) by High Pressure Processing (HPP). International Food Research Journal (IFRJ), 24 (Suppl.). S340-S347. E-ISSN 2231-7546 http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(13)%20R1.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
SH151 Aquaculture - Fish Culture
spellingShingle QD Chemistry
SH151 Aquaculture - Fish Culture
Yusof, N.
Jaswir, Irwandi
Jamal, Parveen
Jami, Mohammed Saedi
Octavianti, Fitri
Optimization of process condition for extraction of gelatin from red tilapia skin (Oreochromis niloticus) by High Pressure Processing (HPP)
description Extraction of gelatin using traditional acid-base pretreatment method has several limitations such as time consuming and causes serious water pollution. Chemical treatment often being used as an alternative process to overcome the weaknesses of the conventional method. However, excessive chemical elements would damage the structure of the gelatin due to its high sensitivity to the acid content. High Pressure Processing (HPP) is a novel and environmental friendly method that has been suggested to assist gelatin extraction. Pressurization during pretreatment could reduce the extraction time and amount of acid used. It also has a potential in enhancing the properties of the gelatin extract and increasing the gelatin yield. In this research, One-Factorat-Time (OFAT) and optimization study were done to determine the optimum parameters for extraction of gelatin assisted by HPP from red tilapia skin. Four parameters; applied pressure, pressure holding time, ratio of acid to skin and extraction time have been selected for the OFAT design and concentration of the gelatin extract and percentage of yield gelatin were evaluated. From OFAT, optimum technical parameters for response surface optimization design were 250 MPa pressure, 7.5 ml of acid to 1 g of skin and 12 hours extraction time. Pressure holding time was fixed for 10 min. FCCCD has been used for optimization study. Results from the data shows that the optimum conditions for gelatin extraction from red tilapia skin were 250 MPa for pressure, 10 min of pressure holding time, 7.5 ml of acid for 1 g of skin and 12 hours of extraction time while the maximum concentration and yield were 19.51 mg/ml and 32.04% (320.4 mg/g), respectively. These findings proved that HPP could increase the concentration and the yield of the gelatin while reducing the chemical waste and shortening the extraction process.
format Article
author Yusof, N.
Jaswir, Irwandi
Jamal, Parveen
Jami, Mohammed Saedi
Octavianti, Fitri
author_facet Yusof, N.
Jaswir, Irwandi
Jamal, Parveen
Jami, Mohammed Saedi
Octavianti, Fitri
author_sort Yusof, N.
title Optimization of process condition for extraction of gelatin from red tilapia skin (Oreochromis niloticus) by High Pressure Processing (HPP)
title_short Optimization of process condition for extraction of gelatin from red tilapia skin (Oreochromis niloticus) by High Pressure Processing (HPP)
title_full Optimization of process condition for extraction of gelatin from red tilapia skin (Oreochromis niloticus) by High Pressure Processing (HPP)
title_fullStr Optimization of process condition for extraction of gelatin from red tilapia skin (Oreochromis niloticus) by High Pressure Processing (HPP)
title_full_unstemmed Optimization of process condition for extraction of gelatin from red tilapia skin (Oreochromis niloticus) by High Pressure Processing (HPP)
title_sort optimization of process condition for extraction of gelatin from red tilapia skin (oreochromis niloticus) by high pressure processing (hpp)
publisher Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
publishDate 2017
url http://irep.iium.edu.my/60862/
http://irep.iium.edu.my/60862/
http://irep.iium.edu.my/60862/1/60862_Optimization%20of%20process%20condition%20for%20extraction.pdf
first_indexed 2023-09-18T21:26:17Z
last_indexed 2023-09-18T21:26:17Z
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